Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Chen, Zhenhai; Yuan, Fuh-Gwo; Lǐ, Yànhuá; Shang, Peng; Schroeder, Robin; Lechtenberg, Kelly F.; Henningson, Jamie; Hause, Ben M.; Bai, Jianfa; Rowland, Raymond R. R.; Clavijo, Alfonso; Fāng, Yīng

doi:10.1016/j.virol.2016.07.003

Cited by 50 publications

(51 citation statements)

References 29 publications

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“…Several recent studies have reported on the pathogenicity of SVA [5,7,[21][22][23][24]. Theoretically, all SVA strains isolated from diseased pigs have pathogenic potentiality.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Zhang

Hao

et al. 2020

Pathogens

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Senecavirus A (SVA), an emerging infectious disease, is associated with the porcine idiopathic vesicular disease. Here, the pathogenesis of different strains of SVA was investigated in growing-finishing pigs. We aimed to evaluate the replication characteristics, virus particle morphology, clinical signs, and vesicular lesions in comparison with two different strains of SVA. The animals were infected with SVA HB-CH-2016 or CH/AH-02/2017 by intranasal routes (3 mL, 109TCID50/mL) and monitored daily for 14 days post-inoculation (dpi) for clinical signs and vesicular lesions. Viremia or viral shedding was detected in the blood, fecal swab, and nasal swab samples. Results showed no distinct differences in plaque size, replication ability, and characteristic virions between SVA HB-CH-2016 and CH/AH-02/2017 strains. Animal experimental results showed that both SVA CH/AH-02/2017 and SVA HB-CH-2016 could infect pigs. However, an obvious difference in the pathogenicity and dynamics of infection was observed between SVA HB-CH-2016 and CH/AH-02/2017 strains. The pathogenesis of SVA CH/AH-02/2017 was similar to that of published results of USA strains, whereas the SVA HB-CH-2016 strain had low pathogenicity to pigs. Clinical signs and vesicular lesions were observed in SVA CH/AH-02/2017-infected pigs. Additionally, the different branches of SVA should be capable of inducing broad cross-reactive neutralizing antibodies, which play an important role in clearing the SVA virus. This study of animal models for SVA infection will be beneficial to develop vaccines and antivirals.

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“…Several recent studies have reported on the pathogenicity of SVA [5,7,[21][22][23][24]. Theoretically, all SVA strains isolated from diseased pigs have pathogenic potentiality.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Zhang

Hao

et al. 2020

Pathogens

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“…Compared with DNA virus, it's discovered that RNA virus, such as influenza virus, coronaviruses, exists a highly evolution rate [27e30]. Previous studies revealed that the prototype SVA isolate, namely SVV-001, was isolated in 2001 [31], and due to its selective tropism for human tumor cells, the virus was developed as an oncolytic agent [32].However, the basic viral pathogenesis was not detected accurately. In this study, the synonymous codon usage of the SVA was analyzed to better understand the SVA evolution, especially the interplay between the viruses and the immune response [33].…”

Section: Discussionmentioning

confidence: 99%

Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Senecavirus A evolution

Chen

Tan

et al. 2017

Microbial Pathogenesis

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Senecavirus A (SVA) infection was recently confirmed in pigs in Brazil, United States of America and Canada. To better understand the molecular characteristics of isolated SVA genomes, we first reported genome-wide comprehensive analyses of codon usage and various factors that have contribute to the molecular evolution in SVA. The effective number of codons (ENC) ranged from 54.51 to 55.54 with an average of 54.87 ± 0.285, which reveals a relatively stable nucleotide composition. We found that codon usage bias of the SVA was low. Mutational pressure acted as an increasingly dominant factor for the evolution of the virus compared with the natural selection. Notably, codon usage bias was also affected by the geographic distribution and isolated time. The first systemic analysis on the codon usage bias of the SVA provides important information for the understanding of the evolution of the SVA and has fundamental and theoretical benefits.

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“…Recently, SVV has been experimentally confirmed to cause vesicular disease in piglets using a contemporary 2015 SVV field isolate from the USA (Chen et al, 2016;Montiel et al, 2016). The clinical signs associated with the vesicular manifestation of SVV infection are indistinguishable from those caused by foot-and-mouth disease (FMD), swine vesicular disease (SVD) and vesicular stomatitis (VS) with pigs presenting with vesicles on the snouts and coronary bands, lameness, anorexia, lethargy, and fever and exclusion of FMD, SVD or VS on affected farms is required by the animal health authorities.…”

Section: Textmentioning

confidence: 99%

Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1 associated with emerging cases of vesicular disease in pigs

Fowler

Ransburgh

Poulsen

et al. 2017

Journal of Virological Methods

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Seneca Valley virus 1 (SVV-1) can cause vesicular disease that is clinically indistinguishable from foot-and-mouth disease, vesicular stomatitis and swine vesicular disease. SVV-1-associated disease has been identified in pigs in several countries, namely USA, Canada, Brazil and China. Diagnostic tests are required to reliably detect this emerging virus, and this report describes the development and evaluation of a novel real-time (r) reverse-transcription (RT) PCR assay (rRT-PCR), targeting the viral polymerase gene (3D) of SVV-1. This new assay detected all historical and contemporary SVV-1 isolates examined (n=8), while no cross-reactivity was observed with nucleic acid templates prepared from other vesicular disease viruses or common swine pathogens. The analytical sensitivity of the rRT-PCR was 0.79 TCID/ml and the limit of detection was equivalent using two different rRT-PCR master-mixes. The performance of the test was further evaluated using pig nasal (n=25) and rectal swab samples (n=25), where concordant results compared to virus sequencing were generated for 43/50 samples. The availability of this assay, will enable laboratories to rapidly detect SVV-1 in cases of vesicular disease in pigs, negated for notifiable diseases, and could enable existing knowledge gaps to be investigated surrounding the natural epidemiology of SVV-1.

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Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Cited by 50 publications

References 29 publications

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Comparison of the Pathogenicity of Two Different Branches of Senecavirus a Strain in China

Genomic analysis of codon usage shows influence of mutation pressure, natural selection, and host features on Senecavirus A evolution

Development of a novel real-time RT-PCR assay to detect Seneca Valley virus-1 associated with emerging cases of vesicular disease in pigs

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