Construction and Characterization of a Bovine Bacterial Artificial Chromosome Library

Cai, Li; Taylor, Jeremy F.; Wing, Rod A.; Gallagher, D. S.; Woo, Sung Sick; Davis, Scott K.

doi:10.1006/geno.1995.9986

Cited by 155 publications

(110 citation statements)

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“…Added to the existing bovine (Cai et al, 1995;Zhu et al, 1999;Buitkamp et al, 2000;Warren et al, 2000;Eggen et al, 2001;Fujisaki et al, 2002), ovine (Gill et al, 1999;Vaiman et al, 1999), and caprine BAC libraries, this library provides improved genome-equivalent coverage of the bovid genomes available.…”

Section: Discussionmentioning

confidence: 99%

“…BAC libraries have been constructed for several species of agricultural importance, including cattle (Cai et al, 1995;Zhu et al, 1999;Buitkamp et al, 2000;Warren et al, 2000;Eggen et al, 2001;Fujisaki et al, 2002), goat , horse (Godard et al, 1998), pig (Rogel-Gaillard et al, 1999Anderson et al, 2000;Suzuki et al, 2000;Fahrenkrug et al, 2001;Jeon et al, 2003;Liu et al, 2010), and sheep (Gill et al, 1999;Vaiman et al, 1999). Although large-insert DNA libraries from closely related ruminant species such as cattle, goat, and sheep have been used for in situ hybridization experiments in buffalo (Di Meo et al, 2005Perucatti et al, 2009), a buffalo library is necessary for whole-genome physical mapping and isolation of genes and gene clusters as well as for the identification of regulatory elements.…”

Section: Introductionmentioning

confidence: 99%

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Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Stafuzza

Abbey²,

Gill

et al. 2012

Genet. Mol. Res.

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ABSTRACT. River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome. This genomic resource for river buffalo will facilitate further studies in this economically important species allowing for instance, whole genome physical mapping and isolation of genes and gene clusters, contributing to the elucidation of gene organization and identification of regulatory elements.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Stafuzza

Abbey²,

Gill

et al. 2012

Genet. Mol. Res.

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“…Methods for constructing libraries in pBAC/oriV should not differ from those in regular BACs (Cai et al 1995;Diaz-Perez et al 1996;Asakawa et al 1997), other than simplifying preparation of large quantities of very pure pBAC/oriV vector. The resulting clones, however, could be transformed either into the standard DH10B, which would not allow DNA amplification, or into the DH10B/trfA derivatives, like JW366, that only on command, that is, when the inducer L-arabinose is added, could provide TrfA function that promotes DNA amplification.…”

Section: Construction and Maintenance Of Genomic Librariesmentioning

confidence: 99%

“…The BAC vectors were extensively used for preparation of DNA libraries, for physical mapping, and for large-scale DNA sequencing efforts. BAC libraries from fungi (Diaz-Perez et al 1996), plants (Woo et al 1994;Mozo et al 1998), mammals (Cai et al 1995;Schibler et al 1998), and human DNA (Kim et al 1996;Asakawa et al 1997) have been constructed. The main advantage of using BACs for genomic library construction was the stability of the large, very low or single-copy (SC) clones.…”

mentioning

confidence: 99%

Conditionally Amplifiable BACs: Switching From Single-Copy to High-Copy Vectors and Genomic Clones

Wild¹,

Hradečná²,

Szybalski³

2002

Genome Res.

165

145

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The widely used, very-low-copy BAC (bacterial artificial chromosome) vectors are the mainstay of present genomic research. The principal advantage of BACs is the high stability of inserted clones, but an important disadvantage is the low yield of DNA, both for vectors alone and when carrying genomic inserts. We describe here a novel class of single-copy/high-copy (SC/HC) pBAC/oriV vectors that retain all the advantages of low-copy BAC vectors, but are endowed with a conditional and tightly controlled oriV/TrfA amplification system that allows: (1) a yield of ∼ 100 copies of the vector per host cell when conditionally induced with L-arabinose, and (2) analogous DNA amplification (only upon induction and with copy number depending on the insert size) of pBAC/oriV clones carrying >100-kb inserts. Amplifiable clones and libraries facilitate high-throughput DNA sequencing and other applications requiring HC plasmid DNA. To turn on DNA amplification, which is driven by the oriV origin of replication, we used copy-up mutations in the gene trfA whose expression was very tightly controlled by the araC-P araBAD promoter/regulator system. This system is inducible by L-arabinose, and could be further regulated by glucose and fucose. Amplification of DNA upon induction with L-arabinose and its modulation by glucose are robust and reliable. Furthermore, we discovered that addition of 0.2% D-glucose to the growth medium helped toward the objective of obtaining a real SC state for all BAC systems, thus enhancing the stability of their maintenance, which became equivalent to cloning into the host chromosome.

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“…Significant problems associated with yeast artificial chromosome libraries, such as insert chimerism and rearrangements, appear to be largely eliminated from BAC resources (3)(4)(5)(6). Furthermore, BACs are proven substrates for sequencing when subcloned into plasmids or M13, or used as a primary template for direct end sequencing and internal nucleotide analysis (7).…”

mentioning

confidence: 99%

High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

Cai

Jing

Irvin

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely mapping to large-scale sequencing, which in turn, require new standards to be met by physical maps and large insert libraries. Bacterial artificial chromosome libraries offer enormous potential as the chosen substrate for both mapping and sequencing studies. Physical mapping, however, has come under some scrutiny as being ''redundant'' in the age of large-scale automated sequencing. We report the development and applications of nonelectrophoretic, optical approaches for high-resolution mapping of bacterial artificial chromosome that offer the potential to complement and thereby advance large-scale sequencing projects.

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Construction and Characterization of a Bovine Bacterial Artificial Chromosome Library

Cited by 155 publications

References 0 publications

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Conditionally Amplifiable BACs: Switching From Single-Copy to High-Copy Vectors and Genomic Clones

High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping

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