Construction and characterisation of near-isogenic<i>Plutella xylostella</i>(Lepidoptera: Plutellidae) strains resistant to Cry1Ac toxin

Zhu, Xun; Lei, Yanyuan; Yang, Yuting; Baxter, Simon W.; Li, Jianhong; Wu, Qingjun; Wang, Shaoli; Xie, Wen; Guo, Zhaojiang; Fu, Wei; Zhang, Youjun

doi:10.1002/ps.3785

Cited by 38 publications

(40 citation statements)

References 65 publications

(126 reference statements)

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“…Since domain II in Cry toxins is the main determinant of their specificity (2), the lack of shared binding sites is also supported by low sequence similarity (Ͻ50%) between domains II of the Cry1Ie2 and Cry1Ab or Cry1Fa toxins. On the basis of its high identity with Cry1Ie2 (Ͼ90%), we expect that Cry1Ie1 would also not have receptors in common with the Cry1Ab toxin, which would explain the lack of cross-resistance to Cry1Ie1 in a Cry1Ab-resistant strain of O. furnacalis (14) and Cry1Ac-resistant strains of H. armigera (16) and Plutella xylostella (15). A lack of shared binding sites between another Cry1I toxin (Cry1Ia7) and Cry1Ab was previously reported qualitatively for BBMV from Lobesia botrana and Earias insulana (11).…”

Section: Discussionmentioning

confidence: 99%

“…Transgenic maize expressing Cry1Ie1 was shown to effectively control O. furnacalis in field trials and displayed toxicity to Cry1Ac-resistant Helicoverpa armigera (13). Furthermore, no cross-resistance to Cry1Ie was detected in a Cry1Ab-resistant strain of O. furnacalis (14) or a Cry1Ac-resistant strain of P. xylostella (15). Expression of the Cry1Ac and Cry1Ie1 toxins in transgenic tobacco produced greater activity against susceptible or Cry1Ac-resistant H. armigera than in plants producing a single toxin (16).…”

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confidence: 99%

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Identification of a New cry1I -Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae

Zhao

Jurat-Fuentes

Abdelgaffar

et al. 2015

Appl Environ Microbiol

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c Pyramiding of diverse cry toxin genes from Bacillus thuringiensis with different modes of action is a desirable strategy to delay the evolution of resistance in the European corn borer (Ostrinia nubilalis). Considering the dependency of susceptibility to Cry toxins on toxin binding to receptors in the midgut of target pests, a diverse mode of action is commonly defined as recognition of unique binding sites in the target insect. In this study, we present a novel cry1Ie toxin gene (cry1Ie2) as a candidate for pyramiding with Cry1Ab or Cry1Fa in corn to control Ostrinia species larvae. The new toxin gene encodes an 81-kDa protein that is processed to a protease-resistant core form of approximately 55 kDa by trypsin digestion. The purified protoxin displayed high toxicity to Ostrinia furnacalis and O. nubilalis larvae but low to no activity against Spodoptera or heliothine species or the coleopteran Tenebrio molitor. Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis during sporulation have been widely used as part of spray products or expressed in transgenic crops to control devastating insect pests (1). The mode of action of Cry toxins includes solubilization and activation in the larval midgut fluids, followed by binding to protein receptors on the midgut epithelium, resulting in toxin insertion into the membrane and the formation of a pore that results in enterocyte death by osmotic shock (2). Alterations in any of these steps could potentially lead to insect resistance, yet high levels of resistance are always linked to alterations in toxin receptor genes (3).Pyramiding of multiple B. thuringiensis toxin genes recognizing distinct binding sites in the midgut of the target pest has been demonstrated to delay the evolution of resistance in transgenic plants (4). On the basis of this strategy, transgenic corn producing combinations of the Cry1Ab or Cry1Fa protein or the chimeric Cry1A.105 protein (domains I and II from Cry1Ab and domain III of Cry1Fa) has been commercialized for control of European corn borer (Ostrinia nubilalis) larvae. However, recent reports support the idea that Cry1Ab, Cry1Fa, and Cry1A.105 have binding sites in the O. nubilalis midgut in common (5). Moreover, the genetic potential to develop resistance to Cry1Ab and Cry1Fa has been demonstrated for strains of O. nubilalis through laboratory selection (6-8). These observations, together with the high adoption of B. thuringiensis-treated corn (Bt corn), currently comprising 80% of the corn planted in the United States (http://www.ers.usda.gov /data-products/adoption-of-genetically-engineered-crops-in-the -us/recent-trends-in-ge-adoption.aspx), underlines the high probability of the development of resistance in O. nubilalis. Identification of new Cry toxins amenable to pyramiding with the Cry1A or Cry1Fa toxin in Bt corn is crucial to the development of novel plants that delay resistance evolution in O. nubilalis.Among the cry genes identified to date (http://www.lifesci .sussex.ac.uk/home/Neil_Crickmore/Bt...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Identification of a New cry1I -Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae

Zhao

Jurat-Fuentes

Abdelgaffar

et al. 2015

Appl Environ Microbiol

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“…One way of reducing this variation is to create near isogenic populations of susceptible and resistant insects through continuous backcrossing. Lei et al [17] produced such a pair of P. xylostella populations and although no biochemical or transcriptomic comparisons were made between them, genetic mapping studies did confirm that resistance was due to a single, autosomal, recessive locus.…”

Section: Alternative Resistance Mechanisms and The Casual Vs Causal Pmentioning

confidence: 99%

Bacillus thuringiensis resistance in Plutella — too many trees?

Crickmore

2016

Current Opinion in Insect Science

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“…and other insects (Tribolium castaneum, Anopheles darlingi, Culex quinquefasciatus, Solenopsis invicta, Apis mellifera, etc.) (49). We examined all search results that correspond to proteins with annotations related to "hypothetical protein" or "putative protein" that could be considered doubtful because of the lack of experimental evidence.…”

Section: Confirmation Of Annotated Protein-coding Genes Inmentioning

confidence: 99%

“…The draft sequence of the P. xylostella genome was released recently (49) and provided the conditions to uncover the molecular function of the larval midgut in P. xylostella. In the present study, we have carried out an in-depth proteomic analysis of midgut proteins of P. xylostella using shotgun nanoLC-MS/MS.…”

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confidence: 99%

Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline

Zhu

Xie

Armengaud

et al. 2016

Molecular & Cellular Proteomics

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The diamondback moth, Plutella xylostella (L.), is the major cosmopolitan pest of brassica and other cruciferous crops. Its larval midgut is a dynamic tissue that interfaces with a wide variety of toxicological and physiological processes. The draft sequence of the P. xylostella genome was recently released, but its annotation remains challenging because of the low sequence coverage of this branch of life and the poor description of exon/intron splicing rules for these insects. Peptide sequencing by computational assignment of tandem mass spectra to genome sequence information provides an experimental independent approach for confirming or refuting protein predictions, a concept that has been termed proteogenomics. In this study, we carried out an in-depth proteogenomic analysis to complement genome annotation of P. xylostella larval midgut based on shotgun HPLC-ESI-MS/MS data by means of a multialgorithm pipeline. A total of 876,341 tandem mass spectra were searched against the predicted P. xylostella protein sequences and a whole-genome six-frame translation database. Based on a data set comprising 2694 novel genome search specific peptides, we discovered 439 novel protein-coding genes and corrected 128 existing gene models. To get the most accurate data to seed further insect genome annotation, more than half of the novel protein-coding genes, i.e. 235 over 439, were further validated after RT-PCR amplification and sequencing of the corresponding transcripts. Furthermore, we validated 53 novel alternative splicings. Finally, a total of 6764 proteins were identified, resulting in one of the most comprehensive proteogenomic study of a nonmodel animal. As the first tissue-specific proteogenomics analysis of P. xylostella, this study provides the fundamental basis for high-throughput proteomics and functional genomics approaches aimed at deciphering the molecular mechanisms of resistance and controlling this pest. Thousands of eukaryotic genomes have been sequenced with the rapid development of massive parallel sequencing technologies. The annotation of their genomic DNA sequences is a prerequisite to delineate conserved elements and understand the specific encoded functions. Today, conventional approaches used to identify protein-coding genes are highly dependent on predictions based on computational algorithms and homology searches against known proteins. Nonmodel organisms, such as most arthropods, are by nature distantly related to well-studied organisms (1). As a result, their genomes encode a large majority of proteins with little similarity to known proteins, challenging the annotation process. Much recent focus on computational gene finding is using transcript evidence to complement this predictionbased approach. Indeed, the use of information from deep sequencing of mRNA-derived cDNA libraries (RNA-seq) or expressed sequence tag (EST) 1 libraries can dramatically improve the genome annotation confidence (2-4). However, this analysis remains at the transcript level and cannot make a crystal-clear distinct...

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Construction and characterisation of near-isogenicPlutella xylostella(Lepidoptera: Plutellidae) strains resistant to Cry1Ac toxin

Cited by 38 publications

References 65 publications

Identification of a New cry1I -Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae

Identification of a New cry1I -Type Gene as a Candidate for Gene Pyramiding in Corn To Control Ostrinia Species Larvae

Bacillus thuringiensis resistance in Plutella — too many trees?

Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline

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