Construction and application of an avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA) for gene expression profiling during Eimeria maxima infection

Kim, Chul Hong; Lillehoj, Hyun S.; Bliss, Travis W.; Keeler, Calvin L.; Hong, Yeong Ho; Park, Dong Woon; Yamage, Mat; Min, Wongi; Lillehoj, Erik P.

doi:10.1016/j.vetimm.2008.04.013

Cited by 21 publications

(23 citation statements)

References 46 publications

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“…Although 3 SNP associated with coccidiosis resistance were located in the TCR-β gene, these SNP were in high LD (r 2 > 0.5) and showed relatively weak evidence for disease resistance association compared with the SNP_892 of the MLF2 gene. In spite of significant associations between MLF2 and coccidiosis resistance, the function of MLF2 is not well understood in host immunity to infectious microorganisms in chickens (Kim et al, 2008). Myeloid leukemia factor proteins are conserved across the mammals and myeloid leukemia factor proteins from nonvertebrate organisms suggest that MLF1 and MLF2 were derived from the duplication of a unique common ancestor (Martin-Lannerée et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Three selected genes, T cell receptor-β (TCR-β), myeloid leukemia factor 2 (MLF2), and lymphotactin, were chosen based upon their genomic location at 80 to 90 cM on chromosome 1 (http://www.ncbi.nlm.nih.gov/ mapview), expression, and function suggesting involvement in protective immunity to coccidiosis. Indeed, the levels of MLF2, TCR-β, and lymphotactin transcripts were increased >2.0-fold at 5 to 6 d postinfection (PI) with coccidia parasites compared with uninfected birds using an avian intestinal intraepithelial lymphocyte cDNA microarray (Kim et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Kim¹,

Hong

Lillehoj³

2010

Poultry Science

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Associations between the parameters of resistance to coccidiosis and SNP in 3 candidate genes located on chromosome 1 [T cell receptor-beta (TCR-beta), myeloid leukemia factor 2 (MLF2), and lymphotactin] were determined. Single nucleotide polymorphisms were genotyped in 24 F1 generation and 290 F2 generation birds. Four SNP were identified in the lymphotactin gene, 12 were located in the TCR-beta gene, and 4 in the MLF2 gene. At various times after experimental infection of the F2 generation with Eimeria maxima, BW, fecal oocyst shedding, and biochemical parameters were measured as parameters of coccidiosis resistance. Single marker association test was applied to determine the associations between the 20 SNP and the parameters of coccidiosis resistance. The maximum additive genetic effect on disease resistance of an SNP in MLF2 was explained by BW (P = 0.0002). The SNP in MLF2 significantly associated with BW was also associated with fecal oocyst shedding (P = 0.001). Four SNP associated with oocyst shedding were found within the coding region of TCR-beta (P < 0.05). Although none of the lymphotactin SNP were associated with oocyst shedding, interferon-gamma level was associated with 2 SNP in lymphotactin. These results suggest that the SNP in the MLF2 gene can be one of the markers to select coccidiosis resistance in chickens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Kim¹,

Hong

Lillehoj³

2010

Poultry Science

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“…According to a reference design with dye swap [31], 4 values were obtained for each treatment. Hybridizations were performed using HybIt hybridization buffer (TeleChem, Sunnyvale, CA) in ArrayIt reaction cassettes at 50°C overnight as described [32]. Each sample had a repeated hybridization using the alternate fluorescent dye between the treatment and control.…”

Section: Methodsmentioning

confidence: 99%

“…GeneSpring GX10 software (Silicon Genetics, Redwood, CA) was used to qualify and normalize image analysis data and to perform the fold-change analyses as described [32]. To generate signal ratios, signal channel values (treatment group) were divided by control channel values (control group).…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens

Lillehoj

Kim

Bravo³

et al. 2011

BMC Proc

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BackgroundThe present study was conducted to investigate the effects of dietary plant-derived phytonutrients, carvacrol, cinnamaldehyde and Capsicum oleoresin, on the translational regulation of genes associated with immunology, physiology and metabolism using high-throughput microarray analysis and in vivo disease challenge model of avian coccidiosis.MethodsIn this study, we used nutrigenomics technology to investigate the molecular and genetic mechanisms of dietary modulation of host innate immunity and metabolism by three phytonutrients. To validate their immunomodulatory effects in a disease model, young broiler chickens fed a standard diet supplemented with three phytochemicals (carvacrol, cinnamaldehyde, and Capsicum oleoresin) from one day post-hatch were orally challenged with E. acervulina. The body weight gain and fecal oocyst production were used to evaluate coccidiosis disease parameters.ResultsAnalysis of global gene expression profiles of intestinal tissues from phytonutrient-fed birds indicated that Capsicum oleoresin induced the most gene changes compared to the control group where many of these genes were associated with those of metabolism and immunity. The most reliable network induced by dietary cinnamaldehyde treatment was related with the functions of antigen presentation, humoral immune response, and inflammatory disease. Furthermore, dietary supplementation with these phytonutrients significantly protected broiler chickens against live coccidiosis challenge infection based on body weight and parasite fecundity.ConclusionsThe results of this study provide clear evidence to support the idea that plant-derived phytochemicals possess immune-enhancing properties in chickens and these new findings create a new possibility to develop effective drug-free alternative strategies for disease control for poultry infectious diseases.

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“…Recent high throughput DNA microarray technology on a whole-genome or tissue-specific basis enables the investigation of complex transcriptional patterns, providing new insights to analyze intricate biological systems, such as host-parasite interactions during coccidiosis [3]. Recently, we constructed a 9.6K intestinal IEL cDNA microarray (AVIELA) which was utilized to show that immune-related genes such as apoptosis as well as the JAK/STAT and MAPK signaling pathways were up- or down-regulated in the jejunum during E. maxima infection [4]. The current study was undertaken using the improved second-generation AVIELA to study local immune responses in chickens infected with a duodenum-infecting coccidia species, E. acervulina , since its host immune response is less known compared to other Eimeria parasites.…”

Section: Introductionmentioning

confidence: 99%

Analysis of global transcriptional responses of chicken following primary and secondary Eimeria acervulina infections

et al. 2011

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BackgroundCharacterization of host transcriptional responses during coccidia infections can provide new clues for the development of alternative disease control strategies against these complex protozoan pathogens.MethodsIn the current study, we compared chicken duodenal transcriptome profiles following primary and secondary infections with Eimeria acervulina using a 9.6K avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA).ResultsGene Ontology analysis showed that primary infection significantly modulated the levels of mRNAs for genes involved in the metabolism of lipids and carbohydrates as well as those for innate immune-related genes. By contrast, secondary infection increased the levels of transcripts encoded by genes related to humoral immunity and reduced the levels of transcripts for the innate immune-related genes. The observed modulation in transcript levels for gene related to energy metabolism and immunity occurred concurrent with the clinical signs of coccidiosis.ConclusionsOur results suggest that altered expression of a specific set of host genes induced by Eimeria infection may be responsible, in part, for the observed reduction in body weight gain and inflammatory gut damage that characterizes avian coccidiosis.

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Construction and application of an avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA) for gene expression profiling during Eimeria maxima infection

Cited by 21 publications

References 46 publications

Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-β on resistance to coccidiosis in chickens

Effects of dietary plant-derived phytonutrients on the genome-wide profiles and coccidiosis resistance in the broiler chickens

Analysis of global transcriptional responses of chicken following primary and secondary Eimeria acervulina infections

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