Construction and Analysis of the Complete Genome Sequence of Leprosy Agent Mycobacterium lepromatosis

Abstract

Leprosy is a dreaded infection that still affects millions of people worldwide. Mycobacterium lepromatosis is a recently recognized cause in addition to the well-known Mycobacterium leprae . M. lepromatosis is likely specific for diffuse lepromatous leprosy, a severe form of the infection and endemic in Mexico. This study constructed and annotated the complete genome sequence of M. lepromatosis FJ924 and performed comparative genomic analyses with related mycobacteria.

Mycobacterium haemophilum infection in canine cerebrospinal fluid

“…5,13 Of all Mycobacterium, neuronal invasion is unique to the M. leprae clade. 6 Central nervous system infection is rare but has been documented in humans, zebrafish, a cat, an American Bison, and a leatherback sea turtle. 9,14,15 Although not a typical route of infection, the neuroanatomical proximity of the historical wooden foreign body, in this case, raises suspicion of mycobacterium inoculation at that time.…”

“…[3][4][5] The M leprae complex is a small clade of fastidious, slowly growing, Mycobacterium species that includes M haemophilum as well as M leprae and M lepromatosis, the agents of human leprosy, and M uberis, found in ruminants. 6,7 Mycobacterium leprae, M lepromatosis, and M uberis have not been cultured axenically, and M haemophilum has an iron uptake deficiency that prevents it from growing on standard mycobacterial media, in addition to growing more optimally at 30-32°C than at 37°C. 8 Culture has been reported to take 3 months or longer.…”

“…Of all Mycobacterium , neuronal invasion is unique to the M . leprae clade 6 . Central nervous system infection is rare but has been documented in humans, zebrafish, a cat, an American Bison, and a leatherback sea turtle 9,14,15 .…”

Genomic analysis of Mycobacterium brumae sustains its nonpathogenic and immunogenic phenotype

“…fallax . Mycobacterium brumae genome is among the smallest mycobacterial genomes, after Mycobacterium uberis ( Benjak et al, 2018 ), Mycobacterium lepromatosis ( Silva et al, 2022 ), and Mycobacterium leprae ( Rahman et al, 2014 ; Matsumoto et al, 2019 ). Our results are highly congruent with the draft genome obtained with an Illumina MiSeq, which had a length of 4,026,006 bp and a mean GC ratio of 69.1% ( D’Auria et al, 2016 ).…”

Genetic Stability ofMycobacterium smegmatisunder the Stress of First-Line Antitubercular Agents: Assessing Mutagenic Potential

“…Davis and Forse compared the sequences of proteins involved in base excision repair and nucleotide excision repair pathways in E. coli and their homologs in M. smegmatis and M. tuberculosis and found that there is a high degree of conservation between the DNA repair enzymes in M. smegmatis and M. tuberculosis (37, 38). Bioinformatic analyses of completely sequenced mycobacterial genomes including M. tuberculosis (39), M. leprae (40), M. bovis (41, 42), M. avium, M. paratuberculosis , and M. smegmatis (43) also demonstrated through the comparison of genes participating in many of the DNA repair/recombination pathways that the basic strategy used to repair DNA lesions is conserved(44, 45). Durbach et.al, investigated mycobacterial SOS response and showed that the M. tuberculosis, M. smegmatis and M. leprae LexA proteins are functionally conserved at the level of DNA binding(46).…”