Construction and analysis of Bordetella pertussis mutants defective in the production of fimbriae

Mooi, Frits R.; Jansen, Wim; Brunings, Henk A.; Gielen, Henk; Heide, Han G. J. van der; Walvoort, H. C.; Guiñée, P. A. M.

doi:10.1016/0882-4010(92)90115-5

Cited by 80 publications

(57 citation statements)

References 17 publications

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“…At each of these time points, however, the fimbrial mutant was able to persist in the nasal cavity at levels similar to wild-type levels. These results confirm and extend previous studies in which it was shown that Fim Ϫ B. pertussis strains were defective in tracheal colonization in mice (18,32). That B. bronchiseptica fimbriae, like B. pertussis fimbriae, contribute to tracheal colonization is further supported by the observation that Fim Ϫ bacteria showed a decreased level of tracheal colonization even in anesthetized animals.…”

Section: Discussionsupporting

confidence: 82%

“…These genes are unlinked on the Bordetella chromosome, and their protein products are 57 to 60% identical at the amino acid level (7,15). Although results from in vitro and in vivo studies with B. pertussis are consistent with the hypothesis that fimbriae contribute to the adherence of Bordetella to respiratory epithelium (32,33), and Fim2 and Fim3 have been included as components of current acellular pertussis vaccines (21), the precise role of fimbriae in pathogenesis has not been conclusively established. A major obstacle has been the lack of a natural animal model for this strictly human pathogen.…”

mentioning

confidence: 61%

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Role of Bordetella bronchiseptica Fimbriae in Tracheal Colonization and Development of a Humoral Immune Response

Mattoo¹,

Miller

Cotter³

2000

Infect Immun

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Fimbriae are filamentous, cell surface structures which have been proposed to mediate attachment of Bordetella species to respiratory epithelium. Bordetella bronchiseptica has four known fimbrial genes: fim2, fim3, fimX, and fimA. While these genes are unlinked on the chromosome, their protein products are assembled and secreted by a single apparatus encoded by the fimBCD locus. The fimBCD locus is embedded within the fha operon, whose genes encode another putative adhesin, filamentous hemagglutinin (FHA). We have constructed a Fim ؊ B. bronchiseptica strain, RB63, by introducing an in-frame deletion extending from fimB through fimD. Western blot analysis showed that RB63 is unable to synthesize fimbriae but is unaffected for FHA expression. Using this mutant, we assessed the role of fimbriae in pathogenesis in vitro and in vivo in natural animal hosts. Although RB63 was not significantly defective in its ability to adhere to various tissue culture cell lines, including human laryngeal HEp-2 cells, it was considerably altered in its ability to cause respiratory tract infections in rats. The number of ⌬fimBCD bacteria recovered from the rat trachea at 10 days postinoculation was significantly decreased compared to that of wild-type B. bronchiseptica and was below the limit of detection at 30 and 60 days postinoculation. The number of bacteria recovered from the nasal cavity and larynx was not significantly different between RB63 and the wild-type strain at any time point. The ability of fimbriae to mediate initial attachment to tracheal tissue was tested in an intratracheal inoculation assay. Significantly fewer RB63 than wild-type bacteria were recovered from the tracheas at 24 h after intratracheal inoculation. These results demonstrate that fimbriae are involved in enhancing the ability of B. bronchiseptica to establish tracheal colonization and are essential for persistent colonization at this site. Interestingly, anti-Bordetella serum immunoglobulin M (IgM) levels were significantly lower in animals infected with RB63 than in animals infected with wild-type B. bronchiseptica at 10 days postinoculation. Even at 30 days postinoculation, RB63-infected animals had lower serum anti-Bordetella antibody titers in general. This disparity in antibody profiles suggests that fimbriae are also important for the induction of a humoral immune response.Specific attachment to host tissues is a crucial event in the initiation of bacterial infections. For many gram-negative bacteria, attachment has been shown to be mediated by filamentous polymeric protein cell surface structures called fimbriae (27). For instance, type IV pili of Neisseria species and Pseudomonas aeruginosa, as well as type I and pyelonephritis-associated P (Pap) pili of Escherichia coli, have been shown to serve as essential adhesins for colonization (for reviews, see references 1, 10, 22, 34, and 39).Bordetella pertussis and Bordetella bronchiseptica are small, aerobic, gram-negative bacteria that colonize the respiratory mucosa of humans and other mammals...

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 61%

Role of Bordetella bronchiseptica Fimbriae in Tracheal Colonization and Development of a Humoral Immune Response

Mattoo¹,

Miller

Cotter³

2000

Infect Immun

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“…fim2 and fim3 encode subunits of the long serrated fimbriae, serotypes 2 and 3, respectively (Heck et al, 1996;Mooi et al, 1987). Fimbriae (also called pili) allow B. pertussis to adhere to host cells and are required for efficient establishment of tracheal colonization and persistence in mouse and rat models (Geuijen et al, 1997;Mooi et al, 1992).…”

Section: Phase Variation In the Fim Genes Generates Phenotypic Diversmentioning

confidence: 99%

The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA

et al. 2012

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“…67 Fim2 and Fim3 have been incorporated into some licensed acellular vaccines; however their inclusion does not appear to augment the protective efficacy of DTaP. 33 In contrast, an early clinical trial of a pertussis whole cell vaccine (WCV) in the UK found a correlation between serum agglutinin titers and protection against whooping cough in infants.…”

mentioning

confidence: 99%

Development of improved vaccines against whooping cough: Current status

Marzouqi¹,

Richmond²,

Fry³

et al. 2010

Human Vaccines

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Construction and analysis of Bordetella pertussis mutants defective in the production of fimbriae

Cited by 80 publications

References 17 publications

Role of Bordetella bronchiseptica Fimbriae in Tracheal Colonization and Development of a Humoral Immune Response

Role of Bordetella bronchiseptica Fimbriae in Tracheal Colonization and Development of a Humoral Immune Response

The Bordetella pertussis model of exquisite gene control by the global transcription factor BvgA

Development of improved vaccines against whooping cough: Current status

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