Construction and analysis of a subtracted library and microarray of cDNAs expressed specifically in chicken heart progenitor cells

Abstract

A subtracted library was constructed of genes expressed specifically in the chick precardiac mesoendoderm. The subtracted library was obtained by hybridization of nucleic acids derived from a starting tester library of stage 4 -7 chick precardiac mesoendoderm and a starting driver library of stage 2 area pellucida. Approximately 11,000 clones from the resulting subtracted library were printed onto a microarray. Screening of the microarray with probes derived from cardiac and noncardiac tissues, followed by in situ hybridization during chick embryo development, has identified multiple cardiac-specific genes, including several that have not been characterized previously. The microarray will be useful for future attempts to identify additional novel cardiac-specific genes, as well as to characterize patterns of gene expression during heart differentiation. Developmental Dynamics 230:290 -298, 2004.

Avian transcriptomics: opportunities and challenges

“…In gene expression studies, using different fluorescent labels for different samples enables the comparison of gene expression between groups and thus differential gene expression. Microarrays have been developed to study the expression of genes involved in a wide range of functions in avian model species, such as bird song and immune reactions (Afrakhte and Schultheiss 2004;Bliss et al 2005;Burnside et al 2005;Li et al 2008;Neiman et al 2001;Smith et al 2006;Van Hemert et al 2003;Wada et al 2006;Wade et al 2004). Microarrays customised for avian model species such as Zebra Finch Taeniopygia guttata (Naurin et al 2008;Wada et al 2006) have further been used for related species (termed 'cross-species microarray analysis') (Cheviron et al 2008;Naurin et al 2012).…”

Multipurpose Instantaneous Microarray Detection of Acute Encephalitis Causing Viruses and Their Expression Profiles

“…Among the most critical are: primer length, melting temperature (T m ), specificity, complementary primer sequences, G/C content and polypyrimidine (T, C) or polypurine (A, G) stretches, and 3 0end sequence; each of these critical elements is discussed in turn [121,127,129]. Practically all viral strains carry a unique DNA sequence that differentiates it from other strains, and this sequence can be used for probe design [1,35]. The probe DNA binds specifically to the target gene corresponding to the viral strain prepared from a clinical sample [31,53,120].…”

Genomics of Congenital Heart Disease

“…Some of these experiments have focused on regional differences in cardiac gene expression in the early vertebrate embryo or in the adult heart ( Afrakhte and Schultheiss, 2004 ;Zhao et al, 2002 ). Some of these experiments have focused on regional differences in cardiac gene expression in the early vertebrate embryo or in the adult heart ( Afrakhte and Schultheiss, 2004 ;Zhao et al, 2002 ).…”