Constructing of DNA vectors with controlled nanosize and single dispersion by block copolymer coating gold nanoparticles as template assembly

Li, Junbo; Wu, Wen-Lan; Jiayu, Gao; Ju, Liang; Zhou, Huiyun; Liang, Lijuan

doi:10.1007/s11051-017-3783-0

Cited by 1 publication

(3 citation statements)

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“…Our previous reports demonstrated that polyplexes [45] and nanoplexes [24] with a PEG shell, and which condense DNA via PAMPImB, are highly stable in physiological media. We assessed magnetic nanoplex stability in different media by DLS (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Vector stability under physiological conditions has significant effects on gene expression in vitro and on further applications in vivo [ 44 ]. Our previous reports demonstrated that polyplexes [ 45 ] and nanoplexes [ 24 ] with a PEG shell, and which condense DNA via PAMPImB, are highly stable in physiological media. We assessed magnetic nanoplex stability in different media by DLS (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Kataoka’s group fabricated a similar NP small vehicle (~50 nm) that showed significant colloidal stability in vivo and high accumulation of siRNA in a cancer model [ 23 ]. Moreover, our group studied a process of DNA loading and the influence of PEG- b -PAMPImB-capped Au NPs on colloid stability during delivery [ 24 ]. These vectors exhibited a mono-disperse state to translocate across the cell membrane and then partly entered the nucleus, thus inducing high and efficient gene expression.…”

Section: Introductionmentioning

confidence: 99%

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Block copolymer conjugated Au-coated Fe3O4 nanoparticles as vectors for enhancing colloidal stability and cellular uptake

Zou

Jiayu

et al. 2017

J Nanobiotechnol

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BackgroundPolymer surface-modified inorganic nanoparticles (NPs) provide a multifunctional platform for assisting gene delivery. Rational structure design for enhancing colloidal stability and cellular uptake is an important strategy in the development of safe and highly efficient gene vectors.ResultsHeterogeneous Au-coated Fe3O4 (Fe3O4@Au) NPs capped by polyethylene glycol-b-poly1-(3-aminopropyl)-3-(2-methacryloyloxy propylimidazolium bromine) (PEG-b-PAMPImB-Fe3O4@Au) were prepared for DNA loading and magnetofection assays. The Au outer shell of the NPs is an effective platform for maintaining the superparamagnetism of Fe3O4 and for PEG-b-PAMPImB binding via Au–S covalent bonds. By forming an electrostatic complex with DNA at the inner PAMPImB shell, the magnetic nanoplexes offer steric protection from the outer corona PEG, thereby promoting high colloidal stability. Transfection efficiency assays in human esophageal cancer cells (EC109) show that the nanoplexes have high transfection efficiency at a short incubation time in the presence of an external magnetic field, due to increased cellular internalization via magnetic acceleration. Finally, after transfection with the magnetic nanoplexes EC109 cells acquire magnetic properties, thus allowing for selective separation of transfected cells.ConclusionPrecisely engineered architectures based on neutral-cationic block copolymer-conjugated heterogeneous NPs provide a valuable strategy for improving the applicability and efficacy of synthesized vectors.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-017-0290-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%