Constructing a novel Nanodevice powered by δ-free FoF1-ATPase

Su, Ting; Cui, Yuanbo; Zhang, Xiao-Ai; Liu, Xiaolong; Yue, Jiachang; Liu, Ning; Jiang, Peidong

doi:10.1016/j.bbrc.2006.09.152

Cited by 20 publications

(14 citation statements)

References 24 publications

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“…Lipidbiotin was purchased from Avanti. The δ-free ATPase within chromatophores was prepared as the previous study [18,19]. The β-subunit of F 0 F 1 -ATPase was expressed and purified as described previously [20].…”

Section: Methodsmentioning

confidence: 99%

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A Novel Biosensor to Detect MicroRNAs Rapidly

2009

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δ-freeF0F1-ATPase within chromatophore was constructed as a novel biosensor to detect miRNA targets. Specific miRNA probes were linked to each rotaryβsubunits ofF0F1-ATPase. Detection of miRNAs was based on the proton flux change induced by light-driven rotation ofδ-freeF0F1-ATPase. The hybridization reaction was indicated by changes in the fluorescent intensity of pH-sensitive CdTe quantum dots. Our results showed that the assay was attomole sensitivities (1.2×10−18 mol) to target miRNAs and capable of distinguishing among miRNA family members. Moreover, the method could be used to monitor real-time hybridization without any complicated fabrication before hybridization. Thus, the rotary biosensor is not only sensitive and specific to detect miRNA target but also easy to perform. Theδ-freeF0F1-ATPase-based rotary biosensor may be a promising tool for the basic research and clinical application of miRNAs.

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Section: Methodsmentioning

confidence: 99%

“…The δfree F o F 1 -ATPase is constructed with a 3 , b 3 , ε, and γ as well as c n subunits as rotator and a, b 2 as stator. Based on this, Su et al constructed a nanomotor by using δ-free F o F 1 -ATPase within chromatophores [18].…”

Section: Introductionmentioning

confidence: 99%

A Novel Biosensor to Detect MicroRNAs Rapidly

2009

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“…The ε-subunit was expressed and purified as in Ref. (Su et al, 2006). The ε-subunit monoclonal antibodies were prepared according to the method for monoclonal antibody production procedure in Ref.…”

Section: Preparation Of Monoclonal Antibodies Of ε-Subunitmentioning

confidence: 99%

microRNA detection with an active nanodevice FoF1-ATPase

Shu

Ouyang

2016

Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies

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A novel nanodevice was constituted with a rotary motor and a "battery", F o F 1-ATPase and chromatophore. The former can processively rotate at about 10 3 r.p.m for more than one hour once the latter was recharged by shine. If the nanodevice is captured by a target such as miRNA and processively rotates for 30 minutes, the number of targets will be amplified by 10 5 ATP molecules. The sensitivity of detection was lower than 1.0 pM. Therefore, this method has potential to be developed into an ultrasensitive biosensor to detect low expressed targets such as miRNA.

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“…Because some α and β subunits may be removed during δ delation, the LiCl-treated proteoliposome needs incubating with purified α 3 β 3 γ(or β) subunits at 4 • C for 60 min (for each divided subunit of F 1 with buffer 0.1 mM Tricine-NaOH, 10 mM MgCl 2 , 50 mM KCl, 0.5 mM NaCl, and 1 mM ATP), and then is cultured at 37 • C for 60 min (for reconstituting of δ-free F o F 1 -ATPase). The proteoliposome needs to be isolated to obtain the reconstituted δ-free F o F 1 -ATPase (Liao et al, 2009;Su et al, 2006;Tao et al, 2008).…”

Section: δ-Free F O F 1 Rotary Motormentioning

confidence: 99%