The present study was conducted to assess knowledge level of fish farmer about scientific fish culture practices. The study was conducted with 90 fish farmers randomly selected from ten blocks of Latur Districts of Maharashtra state. Fish farmers of Latur districts were of comparatively middle age, education up to XII STD, low income level, with moderate family size. The present study revealed that majority of farmers were having low level of knowledge related to pond manuring (53%), feed management (45.55%), water quality management (87.77%), health management (92.22%), marketing management (68.88%). In case of seed stocking, majority of farmers were having moderate level of knowledge (45.55%). The knowledge about fish seed stocking and Training are positively correlated. The overall level of knowledge about scientific fish culture practices is poor which might be due to lack of awareness of technology and recently started fish culture enterprises. It is essential to provide technical knowledge through conducting training programme, farmer meet, workshop, field demonstrations to the fish farmers.