Constitutive toxin production under various nitrogen and phosphorus regimes of three ecotypes of Cylindrospermopsis raciborskii ((Wołoszyńska) Seenayya et Subba Raju)

Willis, Anusuya; Adams, Matthew; Chuang, Ann; Orr, Philip T.; O’Brien, Katherine R.; Burford, Michele A.

doi:10.1016/j.hal.2015.05.011

Cited by 69 publications

(57 citation statements)

References 50 publications

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“…For the control (Fig. The growth rates of the CS-506 and CS-509 strains in this study were lower than previously reported under either Nfree or fully nitrate-replete conditions (Saker et al, 1999;Pierangelini et al, 2014;Willis et al, 2015). Both strains generally showed comparable growth patterns and cell yields in the identical growth period, implying that there would non-discernible difference in CS-506 and CS-509 populations under nutrient-sufficient conditions.…”

Section: R Raciborskii Growthcontrasting

confidence: 54%

“…As shown in Supporting Information Table S1, the highest values of average trichome length, vegetative cell number per trichome and overall Chl a content were observed in CS-506 and CS-509 strains cultured in the JM*(+N, 1000) medium with gradual diminution in average trichome length and average heterocyst number per trichome with decreasing Fe concentration under N-free conditions. Similarly, the toxic CS-505 strain had shorter trichomes than non-toxic CS-510 under N-deficient conditions (Willis et al, 2015). In contrast to the similar overall Chl a content of both strains under N-free conditions, the overall Chl a content of the CS-509 strain in JM*(+N, 1000) medium was slightly higher than that for the CS-506 strain.…”

Section: Physiological Characteristicsmentioning

confidence: 81%

“…In contrast, the lower Chl a content of both strains under N-free conditions was stable for different Fe availabilities (50-1000 nM). Indeed, shorter R. raciborskii trichomes were expected in the N-free media, since strains with long trichomes have lower surface area to volume ratios and consequently are at a disadvantage with regard to supplying N (in the form of compounds such as glutamine) to neighbouring vegetative cells under Ndeficient conditions (Bothe et al, 2010;Plominsky et al, 2015;Willis et al, 2015;Burford et al, 2018). Moreover, the straight trichome of CS-509 in the medium with 200 and 1000 nM Fe was generally longer than the coiled trichome of CS-506.…”

Section: Physiological Characteristicsmentioning

confidence: 99%

“…Both strains were maintained in the slightly modified Jaworski's Medium (JM* medium) detailed in SI2 and Supporting Information (Willis et al, 2015(Willis et al, , 2016a. Both strains were maintained in the slightly modified Jaworski's Medium (JM* medium) detailed in SI2 and Supporting Information (Willis et al, 2015(Willis et al, , 2016a.…”

Section: Culture Conditionsmentioning

confidence: 99%

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Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

Yeung

Fujii

et al. 2019

Environmental Microbiology

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Summary The cyanobacterium Raphidiopsis raciborskii is of environmental and social concern in view of its toxicity, bloom‐forming characteristics and increasingly widespread occurrence. However, while availability of macronutrients and micronutrients such as N and Fe are critically important for the growth and metabolism of this organism, the physiological response of toxic and non‐toxic strains of R. raciborskii to varying Fe and N availabilities remains unclear. By determining physiological parameters as a function of Fe and N availability, we demonstrate that R. raciborskii growth and N2‐fixing activity are facilitated at higher Fe availability under N2‐limited conditions with faster growth of the CS‐506 (cylindrospermopsin‐producing) strain compared with that of CS‐509 (the non‐toxic) strain. Radiolabelled Fe uptake assays indicated that R. raciborskii acclimated under Fe‐limited conditions acquires Fe at significantly higher rates than under Fe replete conditions, principally via unchelated Fe(II) generated as a result of photoreduction of complexed Fe(III). While N2‐fixation of both strains occurred during both day and night, the CS‐506 strain overall exhibited higher N2‐fixing and Fe uptake rates than the CS‐509 strain under N‐deficient and Fe‐limited conditions. The findings of this study highlight that Fe availability is of significance for the ecological advantage of CS‐506 over CS‐509 in N‐deficient freshwaters.

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Section: R Raciborskii Growthcontrasting

confidence: 54%

Section: Physiological Characteristicsmentioning

confidence: 81%

Section: Physiological Characteristicsmentioning

confidence: 99%

Section: Culture Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

Yeung

Fujii

et al. 2019

Environmental Microbiology

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“…The average growth rate of the investigated C. raciborskii (0.31 per day) is at the lower end of the spectrum found in previous studies (0.2–1.0 per day; e.g., Bonilla et al., ; Briand et al., ; Isvánovics et al., ; Mehnert et al., ; Thomas & Litchman, ; Willis et al., ). One likely reason is that growth conditions, even in the semi‐continuous experiment, were sub‐optimal because of moderate P‐limitation (80 μg/L) and a temperature (20°C) below the optimal growth temperature for isolates originating from temperate regions (Briand et al., ; Mehnert et al., ).…”

Section: Discussionmentioning

confidence: 47%

High local trait variability in a globally invasive cyanobacterium

2017

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During the last couple of decades, invasive species have become a worldwide problem in many freshwater systems. Besides higher plants and animals, microbes, in particular the potentially toxic cyanobacterium Cylindrospermopsis raciborskii, has attracted increasing attention, due to its spread towards temperate zones of the northern and southern hemisphere. A number of advantageous functional traits and a high intraspecific plasticity have been suggested to explain its invasion success. The aim of this study was to examine intraspecific functional trait variability in 12 different isolates of C. raciborskii originating from different lakes in an invaded region in Northeast Germany. We measured growth rate, C:N:P ratios, chlorophyll‐a content and the abundance of heterocysts under nutrient‐replete and phosphorus‐limited conditions. Moreover, the isolate‐specific morphology and grazing losses by an herbivorous rotifer, as a top‐down force, were studied. DNA fingerprinting revealed that all isolates were genetically different. C. raciborskii exhibited a large variability in all measured traits among isolates. The C:P, N:P and Chl‐a:C ratios differed by a factor of two or more. The trait variability among isolates was higher under nutrient‐replete conditions, except for the C:P ratio, which varied most during phosphorus limitation. The susceptibility to grazing, calculated as maximum ingestion rates of the rotifer Brachionus calyciflorus on C. raciborskii, varied most among isolates, but was not related to any of the measured physiological or morphological traits, i.e. no trade‐off was found. Ecological and genetic clustering did not match, indicating that the genetic relationship based on DNA fingerprinting did not cover ecological differences. Our results show a high trait variability within locally occurring and partly co‐occurring C. raciborskii isolates. No overall trade‐offs between the measured functional traits were found. This demonstrates the ecological relevance of linking multiple traits, e.g. competitive and consumptive. Furthermore, this study emphasises the importance of analysing more than one strain of a species, as different strains show different trait values potentially relevant for their invasibility and the field of general trait‐based ecology.

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Diazotrophy modulates cyanobacteria stoichiometry through functional traits that determine bloom magnitude and toxin production

Wagner

Osburn

Taylor

et al. 2022

Limnology & Oceanography

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Harmful cyanobacterial blooms are an increasing threat to water quality. The interactions between two ecophysiological functional traits of cyanobacteria, diazotrophy (nitrogen [N]‐fixation) and N‐rich cyanotoxin synthesis, have never been examined in a stoichiometric explicit manner. We explored how a gradient of resource N : phosphorus (P) affects the biomass, N, P stoichiometry, light‐harvesting pigments, and cylindrospermopsin production in a N‐fixing cyanobacterium, Aphanizomenon. Low N : P Aphanizomenon cultures produced the same biomass as populations grown in high N : P cultures. The biomass accumulation determined by carbon, indicated low N : P Aphanizomenon cultures did not have a N‐fixation growth trade‐off, in contrast to some other diazotrophs that maintain stoichiometric N homeostasis at the expense of growth. However, N‐fixing Aphanizomenon populations produced less particulate cylindrospermopsin and had undetectable dissolved cylindrospermopsin compared to non‐N‐fixing populations. The pattern of low to high cyanotoxin cell quotas across an N : P gradient in the diazotrophic cylindrospermopsin producer is similar to the cyanotoxin cell quota response in nondiazotrophic cyanobacteria. We suggest that diazotrophic cyanobacteria may be characterized into two broad functional groups, the N‐storage‐strategists and the growth‐strategists, which use N‐fixation differently and may determine patterns of bloom magnitude and toxin production in nature.

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Constitutive toxin production under various nitrogen and phosphorus regimes of three ecotypes of Cylindrospermopsis raciborskii ((Wołoszyńska) Seenayya et Subba Raju)

Cited by 69 publications

References 50 publications

Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

High local trait variability in a globally invasive cyanobacterium

Diazotrophy modulates cyanobacteria stoichiometry through functional traits that determine bloom magnitude and toxin production

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Constitutive toxin production under various nitrogen and phosphorus regimes of three ecotypes of Cylindrospermopsis raciborskii ((Wołoszyńska) Seenayya et Subba Raju)

Cited by 69 publications

References 50 publications

Physiological responses of the freshwater N2‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

Physiological responses of the freshwater N2‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

High local trait variability in a globally invasive cyanobacterium

Diazotrophy modulates cyanobacteria stoichiometry through functional traits that determine bloom magnitude and toxin production

Contact Info

Product

Resources

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Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities

Physiological responses of the freshwater N₂‐fixing cyanobacterium Raphidiopsis raciborskii to Fe and N availabilities