Constitutive Phenotypic Modification of Lipid A in Clinical Acinetobacter baumannii Isolates

Kim, Suhyun; Yun, Sohyeon; Park, Woojun

doi:10.1128/spectrum.01295-22

Cited by 8 publications

(4 citation statements)

References 54 publications

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“…While the MIC value of doxycycline ranged from (4 -128 mg /ml ) this result agreed with the result by (Wang et al, 2023). Who reported in their study the mic value of doxycycline between (1≥ 128), while the result disagreed with (Kim et al ., 2022).…”

Section: Antibioticresistance Of Acinetobacter Baumannii Isolatessupporting

confidence: 88%

Detection of antibiotics resistance andefflux pumps production in clinical Acinetobacter baumannii isolates

A. Abdulkareem,

Mubarak

2023

JANS

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Acinetobacter baumannii is considered one of the important pathogenic bacteria and responsible for several nosocomial and community infections in humans, such as the lungs (pneumonia), the bloodstream of ICU patients, burns, surgical wounds and meningitis. During the period from September to the end of December 2022, a total of 210 blood specimens were collected inDiyala governorate Baqubah Teaching Hospital, Iraq.To investigate the prevalence of bacterial isolates among ICU patients by identifying bacteria depending on suitable media under suitable environmental conditions for growth, morphological characteristics, biochemical tests, and confirmation of identification isolates by molecular detection of blaOXA- 51gene, antibiotic resistance, phenotypic and molecular detection of efflux pumps adeF gene, and activity of ciprofloxacin andresveratrol on gene expression. The percentage positive growth of A. baumannii isolates from blood specimens among ICU patients was 12 %, and the highest resistance of 25 isolates against fourteen types of antibiotics was equal to cefotaxime (100 % ), ticarcillin-clavulanate, and ceftriaxone ( 96 %), and ( 92 % )for each of Cefepime and Tobramycin, the lowest percentage was for doxycycline (64 % ).MIC value for ciprofloxacin and doxycycline between (8- 512 mg/ml) and ( 4 - 128 mg/ml) respectively. Molecular studies indicate the presence of blaOXA- 51gene and adeF in all isolates under study ( 100 % ). The treatment of resistance isolates with ciprofloxacin, resveratrol and their combination resulted in reduction of adeF gene. The resveratrol effect on efflux pumps gene adeF gene expression occurred for the first time in Iraq and even at the level of the Arab world.

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Section: Antibioticresistance Of Acinetobacter Baumannii Isolatessupporting

confidence: 88%

Detection of antibiotics resistance andefflux pumps production in clinical Acinetobacter baumannii isolates

A. Abdulkareem,

Mubarak

2023

JANS

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“… 50 Recently, a constitutive phenotypic modification of lipid A by phosphoethanolamine (PEtN) incorporation was noticed in the multidrug-resistant (MDR) strains of carbapenem-resistant A. baumannii using MALDI-TOF MS, particularly in the polymyxin B resistant strains. 51 There are several advantages of using this method, including the fact that it is very precise and sensitive as well as being capable of identifying whole bacteria cells, as well as bacteria isolates. There is a great deal of success being achieved with MALDI-TOF MS for the identification and typing of microorganisms down to the subspecies level, Further, bacteria cultures that are difficult to grow can also be used to identify them, and as a result, the method is not sensitive to modifications made in microorganism growth protocols prior to analysis.…”

Section: Matrix-assisted Laser Desorption/ionization Time-of-flight M...mentioning

confidence: 99%

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Anwer

2024

SMJ

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“…After 24 h post infection, G. mellonella larvae were each disinfected with 70% ethanol and homogenized in 2 mL of sterile PBS (Dulbecco's Phosphate-Buffered Saline, GenDEPOT) using a high-speed homogenizer (TissueRuptor, Qiagen). The homogenate was serially diluted in and plated on LB agar supplemented with streptomycin (2 μg/mL) and colistin (4 μg/mL) for E. coli FORC81, and gentamicin (256 μg/mL) for A. baumannii NCCP 15989 based on (Kim et al, 2019(Kim et al, , 2022. Bacterial loads were counted and the Lys1S-L9P treated group was analyzed based on the control group with the independent Student's t-tests using GraphPad Prism software.…”

Section: Galleria Mellonella As An Infection Modelmentioning

confidence: 99%

Development of sensitizer peptide-fused endolysin Lys1S-L9P acting against multidrug-resistant gram-negative bacteria

Son,

Kim,

Ryu

2023

Front. Microbiol.

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The advent of multidrug-resistant (MDR) bacteria poses a major threat to public health, garnering attention to novel antibiotic replacements. Endolysin, a bacteriophage-derived cell wall-degrading enzyme, is a promising alternative to conventional antibiotics. However, it is challenging to control Gram-negative bacteria due to the presence of the outer membrane that shields the peptidoglycan layer from enzymatic degradation. To overcome this threshold, we constructed the fusion endolysin Lys1S-L9P by combining endolysin LysSPN1S with KL-L9P, a sensitizer peptide known to extend efficacy of antibiotics by perturbing the outer membrane of Gram-negative bacteria. In addition, we established a new endolysin purification procedure that increases solubility allowing a 4-fold increase in production yield of Lys1S-L9P. The sensitizer peptide-fused endolysin Lys1S-L9P exhibited high bactericidal effects against many MDR Gram-negative pathogens and was more effective in eradicating biofilms compared to LysSPN1S. Moreover, Lys1S-L9P showed potential for clinical use, maintaining stability at various storage temperatures without cytotoxicity against human cells. In the in vivo Galleria mellonella model, Lys1S-L9P demonstrated potent antibacterial activity against MDR Gram-negative bacteria without inducing any toxic activity. This study suggest that Lys1S-L9P could be a potential biocontrol agent to combat MDR Gram-negative bacteria.

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Constitutive Phenotypic Modification of Lipid A in Clinical Acinetobacter baumannii Isolates

Cited by 8 publications

References 54 publications

Detection of antibiotics resistance andefflux pumps production in clinical Acinetobacter baumannii isolates

Detection of antibiotics resistance andefflux pumps production in clinical Acinetobacter baumannii isolates

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Development of sensitizer peptide-fused endolysin Lys1S-L9P acting against multidrug-resistant gram-negative bacteria

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