Constitutive Mucin Secretion Linked to CFTR Expression

Kuver, Rahul; Ramakrishnan, Nagarajan; Lau, Stella; Savard, Christopher E.; Lee, S.P.; Osborne, W.R.A.

doi:10.1006/bbrc.1994.2348

Cited by 44 publications

(28 citation statements)

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“…A role for CFTR in cAMP-and chloride-dependent exocytosis and endocytosis was demonstrated for the wild-type, but not for a defective, chloride channel (43) and may be attributed to an association of the vesicle fusion process with electrolyte transport (44). A combined secretion of mucus and chloride upon CFTR stimulation was demonstrated in subepidermal glands of Xenopus laevis by Engelhardt et al (41) and by studies on submandibular (45), pancreatic epithelial (42), gallbladder (46), and submucosal glands (47), which suggested a role for CFTR in mucus release. Alterations in the glycan composition of CF mucins may result from defective CFTR expression in mucus cells (39).…”

Section: Discussionmentioning

confidence: 96%

ΔF508 CFTR protein expression in tissues from patients with cystic fibrosis

Kälin¹,

Claass²,

Sommer³

et al. 1999

J. Clin. Invest.

249

179

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Section: Discussionmentioning

confidence: 96%

ΔF508 CFTR protein expression in tissues from patients with cystic fibrosis

Kälin¹,

Claass²,

Sommer³

et al. 1999

J. Clin. Invest.

249

179

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“…These animals had less mucus obstruction and longer survival compared with Muc1-expressing littermates (453). In addition, CFTR has been demonstrated to play an important role in regulation of mucus secretion (357,418,424,459). CFTR is expressed in mucous cells of tracheal glands, pancreas, gallbladder, and intestinal tract (177, 461,674).…”

Section: J Secretion Of Mucusmentioning

confidence: 99%

Electrolyte Transport in the Mammalian Colon: Mechanisms and Implications for Disease

Kunzelmann

Mall

2002

Physiological Reviews

580

571

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The colonic epithelium has both absorptive and secretory functions. The transport is characterized by a net absorption of NaCl, short-chain fatty acids (SCFA), and water, allowing extrusion of a feces with very little water and salt content. In addition, the epithelium does secret mucus, bicarbonate, and KCl. Polarized distribution of transport proteins in both luminal and basolateral membranes enables efficient salt transport in both directions, probably even within an individual cell. Meanwhile, most of the participating transport proteins have been identified, and their function has been studied in detail. Absorption of NaCl is a rather steady process that is controlled by steroid hormones regulating the expression of epithelial Na+ channels (ENaC), the Na+-K+-ATPase, and additional modulating factors such as the serum- and glucocorticoid-regulated kinase SGK. Acute regulation of absorption may occur by a Na+ feedback mechanism and the cystic fibrosis transmembrane conductance regulator (CFTR). Cl− secretion in the adult colon relies on luminal CFTR, which is a cAMP-regulated Cl− channel and a regulator of other transport proteins. As a consequence, mutations in CFTR result in both impaired Cl− secretion and enhanced Na+ absorption in the colon of cystic fibrosis (CF) patients. Ca2+- and cAMP-activated basolateral K+ channels support both secretion and absorption of electrolytes and work in concert with additional regulatory proteins, which determine their functional and pharmacological profile. Knowledge of the mechanisms of electrolyte transport in the colon enables the development of new strategies for the treatment of CF and secretory diarrhea. It will also lead to a better understanding of the pathophysiological events during inflammatory bowel disease and development of colonic carcinoma.

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“…DGBE cells and human CaCo-2 cells were cultured in Dulbecco's modified Eagle's medium (9). To determine transduction of dividing cells, 2 ϫ 10 5 DGBE cells or CaCo-2 cells were infected at a multiplicity of infection of 2 for 4 hrs in the presence of 10g/ml DEAE dextran.…”

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confidence: 99%