Constitutive Interferon-Inducible Protein 16-Inflammasome Activation during Epstein-Barr Virus Latency I, II, and III in B and Epithelial Cells

Ansari, Mairaj Ahmed; Singh, Vivek Vikram; Dutta, Sandeep; Veettil, Mohanan Valiya; Dutta, Dipanjan; Leela, Chikoti; Lu, Jie; Everly, David N.; Chandran, Bala

doi:10.1128/jvi.00805-13

Cited by 179 publications

(165 citation statements)

References 56 publications

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“…As with AIM2, the sequence independent DNA binding by IFI16 is achieved by electrostatic attraction between positively charged HIN domain residues and the dsDNA sugar phosphate backbone [41]. This allows IFI16 to detect dsDNA from many sources including Kaposi's sarcoma-related herpesvirus (KSHV) [11], Epstein-Barr virus (EBV) [44], herpes simplex virus 1 (HSV-1) [45] and human immunodeficiency virus (HIV-1) [46]. On the contrary, dsDNA sensing by IFI16 is length dependent [2], with dsDNA fragments of 150 base pairs reportedly being favourable for the formation of an optimal binding cluster of approximately ten IFI16 protomers [47].…”

Section: Discovery Of Aim2 As a Dna Receptormentioning

confidence: 99%

“…However, abortive infection of CD4 + T cells with HIV, which leads to accumulation of HIV DNA, causes IFI16-dependent pyroptosis [51], since like AIM2, IFI16 activation can also promote inflammasome activation in some contexts. IFI16-dependent inflammasome activation has been reported to also occur after infection with herpes viruses such as KSHV, HSV-1 and EBV [11,44,45]. However, HSV-1 and HCMV infection can also result in IFI16 dependent IFN-b induction via the STING/TBK1 pathway.…”

Section: Discovery Of Aim2 As a Dna Receptormentioning

confidence: 99%

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The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease

Connolly

Bowie

2014

Biochemical Pharmacology

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Section: Discovery Of Aim2 As a Dna Receptormentioning

confidence: 99%

Section: Discovery Of Aim2 As a Dna Receptormentioning

confidence: 99%

The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease

Connolly

Bowie

2014

Biochemical Pharmacology

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“…Serological tests are used in clinics to diagnose EBV, but heterophile antibodies are non-specific and not produced in some patients (17). HCMV is the major cause of congenital infection, affecting 0.3% to 2.3% of newborns, that can lead to severe neurological sequelae such as hearing loss and congenital defects (20,21). It also infects the immunocompromised individuals causing fatal pneumonia and colitis.…”

Section: Discussionmentioning

confidence: 99%

Rapid Etiologic Diagnosis of Herpes Virus Infections in Children

Liu

Shen

et al. 2017

Iran J Pediatr

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Background: Human herpes viruses, as common viruses, not only affect mainly the skin, mucosa, and nervous tissue, but also can cause a variety of serious diseases in children. Objectives: The aim of this study was to determine the sensitivity and specificity of multiplex PCR-based DNA microarray technology in comparison with PCR method and IgM ELISA. Methods: A total of 108 blood samples from children with viral infections were collected and analyzed by multiplex PCR-based DNA microarray technology, PCR method, and IgM ELISA. Results: Of 108 specimens, 16 were positive which gave a positive rate of 14.8%. Most of the patients were infected with EBV and HCMV. The sensitivity and specificity of this technology for detecting human herpes viruses were 100% when compared to PCR method. The crude agreement between multiplex PCR-based DNA microarray technology and IgM ELISA for detecting human herpes viruses was 95.4%. Conclusions:The results indicated that multiplex PCR-based DNA microarray technology is a rapid auxiliary diagnostic method for simultaneous detection of the seven common herpes viruses with high sensitivity and specificity.

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“…The detected association between clinical staging and pre-treatment plasma EBV DNA viral load on one side and pre-treatment serum cytokines was approved experimentally by Zhang et al (24) who reported that EBV-infected nasopharyngeal epithelial cells exhibited enhanced response to IL-6-induced STAT-3 activation through overexpression of the IL-6 receptor, thus promoting growth and invasive properties EBV-positive NPC cells. Ansari et al (25) observed that EBV facilitates its genome persistence and evasion of host immune responses through activation of caspase-1, which cleaves the pro-forms of inflammatory IL-1β, IL-18 and IL-33 cytokines. Song et al (26) suggested that TNF-α may be a promoter for NPC local spread and metastasis through the induction of inhibitor of apoptosis proteins, which contribute to both tumor Table II.…”

Section: Discussionmentioning

confidence: 99%

Pre-treatment serum inflammatory cytokines as survival predictors of patients with nasopharyngeal carcinoma receiving chemoradiotherapy

Al-Kholy

Abdullah

Abadier

et al. 2016

Molecular and Clinical Oncology

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Abstract. The present study aimed to examine the predictability of pre-treatment serum levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α for determining the outcome of patients with nasopharyngeal carcinoma (NPC) assigned for chemoradiotherapy. A total of 35 patients with NPC were subjected to clinical examination and evaluation of performance status using Karnofsky scoring. Nasopharyngoscopy was performed for evaluation and to obtain a biopsy. Blood samples were obtained pre-and post-treatment for polymerase chain reaction quantitative estimation of Epstein-Barr virus (EBV) DNA plasma load and enzyme-linked immunosorbent assay for estimation of serum cytokines. All patients received chemoradiotherapy and were followed-up for 2 years. Cervical lymphadenopathy and recurrent attacks of epistaxis are the most common presenting symptoms. Treatment significantly decreased pre-treatment plasma EBV DNA load and serum levels of IL-6 and TNF-α, and increased serum IL-1β levels. Clinical staging and EBV DNA plasma load revealed positively significant correlation with pre-treatment serum levels of both IL-6 and TNF-α, while revealed negative significant correlation with serum IL-1β levels. The 2-year survival rate was negatively significantly correlated with pre-treatment levels of IL-6 and TNF-α, and EBV DNA viral load, while it was positively significantly correlated with pre-treatment performance scores and serum IL-1β levels. Statistical analyses defined high pre-treatment serum IL-6 levels as a significant specific predictor for high mortality rate. It was demonstrated that NPC was associated with high pre-treatment plasma EBV DNA load and serum cytokines, and chemoradiotherapy significantly reduced these high levels. High pre-treatment serum IL-6 level was a significant specific predictor for high mortality rate. Increased post-treatment serum levels of IL-1β indicated good therapeutic response and most probably a high survival rate.

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Constitutive Interferon-Inducible Protein 16-Inflammasome Activation during Epstein-Barr Virus Latency I, II, and III in B and Epithelial Cells

Cited by 179 publications

References 56 publications

The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease

The emerging role of human PYHIN proteins in innate immunity: Implications for health and disease

Rapid Etiologic Diagnosis of Herpes Virus Infections in Children

Pre-treatment serum inflammatory cytokines as survival predictors of patients with nasopharyngeal carcinoma receiving chemoradiotherapy

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