CONSTITUTIVE EXPRESSION OF VARIOUS XENOBIOTIC AND ENDOBIOTIC TRANSPORTER mRNAs IN THE CHOROID PLEXUS OF RATS

Abstract

ABSTRACT:The aim of this study was to quantitatively determine the constitutive expression levels of various transporter mRNAs in rat choroid plexus. To provide a reference for the relative expression levels, the expression of various transporter mRNAs in choroid plexus were compared with that in liver, kidney, and ileum. The mRNA levels of multidrug resistance protein (Mrp)1, 2, 3, 4, 5, and 6; multidrug resistance (Mdr)1a, 1b, and 2; organic anion transporting polypeptide ( The target-organ toxicity of any xenobiotic is dependent on its absorption, distribution, metabolism and excretion (ADME). The topic of xenobiotic metabolism has been the subject of intensive research for over 40 years, but research on various transport proteins that can mediate absorption, distribution, and excretion is a relatively recent undertaking.Studies using rodent and human tissues have reported the existence of specific proteins that transport a variety of xenobiotics and endobiotics, such as drugs, flavonoids, metals, nucleotides and nucleosides, di-and tripeptides, and bile acids (Saito et al

Molecular and biological characterization of the Amblyomma americanum organic anion transporter polypeptide

“…This might explain the seemingly lack of impact of AamOatp silencing on early tick-feeding parameters, attachment onto host skin and beginning to feed. Consistent with vertebrate Oatp/OATP genes that show multiple tissue expression patterns (St-Pierre et al, 2002;Gao et al, 2005;Choudhuri et al, 2003), our RT-PCR expression analyses data revealed that AamOatp mRNA is both constitutively and ubiquitously expressed, as it was it is expressed in all tested organs of ticks that fed for 1-7 days. Transcriptional regulation of tick genes has been shown in response to blood meal feeding, being either shut down or induced, up-or down regulated (Leboule et al, 2002a;Leboule et al, 2002b;Mulenga et al, 2001;Rudenko et al, 2005).…”

Impaired Monoamine and Organic Cation Uptake in Choroid Plexus in Mice with Targeted Disruption of the Plasma Membrane Monoamine Transporter (Slc29a4) Gene

“…However, there is no direct evidence demonstrating the expression of OCT2 in the CP or its localization to the apical membrane. Gene profiling work by us and others showed very low expression of Oct2 in rodent CPs (35). Here, using quantitative real time PCR, we clearly showed that in both human and mouse CP tissues, PMAT/Pmat is the dominating monoamine/OC transporter, whereas other functionally related transporters, including OCT1, OCT2, OCT3, MATE1/2, SERT, DAT, and NET, are minimally expressed (Fig.…”

Bile Acid Signaling Is Involved in the Neurological Decline in a Murine Model of Acute Liver Failure

“…23 Similarly, a diet enriched with 0.3% DCA predominantly increased cholic acid and the taurine conjugates of both species. 52 Therefore, it is reasonable to assume that the effects of cholic acid or DCA feeding on the neurological decline because of acute liver failure may not solely be because of the The Mechanism of Action of Bile Acids in the Brain Involves ASBT-Dependent Uptake and Activation of FXR Circulating bile acids can gain access to the brain via two known mechanisms. First, bile acids can be transported across the BBB through the bile acid transporters found in the choroid plexus.…”

“…First, bile acids can be transported across the BBB through the bile acid transporters found in the choroid plexus. 52 Second, another route of entry is via the diffusion of bile acids through an increasingly permeable BBB. We, and others, have demonstrated that the BBB is impaired in models of acute and chronic liver damage.…”