Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex

Armstrong, Carol L.; Krueger-Naug, Anne Marie R.; Currie, R. William; Hawkes, Richard

doi:10.1002/(sici)1096-9861(20000117)416:3<383::aid-cne9>3.0.co;2-m

Cited by 143 publications

(194 citation statements)

References 68 publications

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“…Sections were cut at 10 -20 m in the transverse or sagittal planes, mounted on gelatin-coated slides, and stained with m10CT-FP at a dilution of 1:200. Indirect peroxidase immunocytochemistry was performed on the slide as described previously (36). Photomicrographs were captured with a SPOT Cooled Color digital camera (Diagnostic Instruments Inc.) and assembled in Adobe Photoshop 4.0.…”

Section: Methodsmentioning

confidence: 99%

Dysbindin, a Novel Coiled-coil-containing Protein That Interacts with the Dystrobrevins in Muscle and Brain

Benson

Newey

Martin‐Rendon

et al. 2001

Journal of Biological Chemistry

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280

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The dystrophin-associated protein complex (DPC) is required for the maintenance of muscle integrity during the mechanical stresses of contraction and relaxation. In addition to providing a membrane scaffold, members of the DPC such as the ␣-dystrobrevin protein family are thought to play an important role in intracellular signal transduction. To gain additional insights into the function of the DPC, we performed a yeast two-hybrid screen for dystrobrevin-interacting proteins. Here we describe the identification of a dysbindin, a novel dystrobrevinbinding protein. Dysbindin is an evolutionary conserved 40-kDa coiled-coil-containing protein that binds to ␣-and ␤-dystrobrevin in muscle and brain. Dystrophin and ␣-dystrobrevin are co-immunoprecipitated with dysbindin, indicating that dysbindin is DPC-associated in muscle. Dysbindin co-localizes with ␣-dystrobrevin at the sarcolemma and is up-regulated in dystrophin-deficient muscle. In the brain, dysbindin is found primarily in axon bundles and especially in certain axon terminals, notably mossy fiber synaptic terminals in the cerebellum and hippocampus. These findings have implications for the molecular pathology of Duchenne muscular dystrophy and may provide an alternative route for anchoring dystrobrevin and the DPC to the muscle membrane.

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Section: Methodsmentioning

confidence: 99%

Dysbindin, a Novel Coiled-coil-containing Protein That Interacts with the Dystrobrevins in Muscle and Brain

Benson

Newey

Martin‐Rendon

et al. 2001

Journal of Biological Chemistry

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280

266

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“…It is possible that the zones in folia that are uniformly ZIIϩ are delineated by other molecular markers. For example, heat shock protein 25 is expressed in stripes in folia VI/VII and X in mammals (Armstrong et al, 2000;Schonewille et al, 2006).…”

Section: The Utility Of the Vestibulocerebellum As A General Model Fomentioning

confidence: 99%

Zebrin-Immunopositive and -Immunonegative Stripe Pairs Represent Functional Units in the Pigeon Vestibulocerebellum

Graham

Wylie

2012

J. Neurosci.

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The cerebellum is a site of complex sensorimotor integration and contains up to 80% of the neurons in the brain, yet comparatively little is known about the organization of sensorimotor systems within the cerebellum. It is known that afferent projections and Purkinje cell (PC) response properties are organized into sagittal "zones" in the cerebellum. Moreover, the isoenzyme aldolase C [also known as zebrin II (ZII)] is heterogeneously expressed in cerebellar PCs such that there are sagittal stripes of PCs with high expression (ZIIϩ) interdigitated with stripes of little or no expression (ZIIϪ). In the present study, we show how the ZII stripes in folium IXcd of the vestibulocerebellum in pigeons are related to response properties of PCs. IXcd consists of seven pairs of ZIIϩ/Ϫ stripes denoted P1ϩ/Ϫ (medial) to P7ϩ/Ϫ (lateral). Electrophysiological studies have shown that vestibulocerebellar PCs respond to particular patterns of optic flow resulting from self-motion in three-dimensional space. In our study, we recorded optic flow preferences from PCs in IXcd, marked recording locations with injections of fluorescent tracer, and subsequently immunoreacted coronal sections for ZII. We found that the PCs within a ZIIϩ/Ϫ stripe pair all responded best to the same pattern of optic flow. That is, a ZIIϩ/Ϫ stripe pair forms a functional unit in the cerebellum. This is the first demonstration that the function of PCs is associated with ZII stripes across the mediolateral extent of an entire folium.

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“…First, the mammalian cerebellum is subdivided into four transverse zones, based on differential gene expression: the anterior zone (AZ: in mouse, ∼lobules I-V in the vermis), the central zone (CZ∼ lobules VI-VII: with two sub-zones (see [6]), the posterior zone (PZ∼lobules VIII-dorsal IX), and the nodular zone (NZ; ventral lobule IX and lobule X: e.g., [4,7]. A fifth zone-the lingular zone-is found in birds [8][9][10] and bats (lobule I: [11]).…”

Section: Introductionmentioning

confidence: 99%

Compartmentation of the Cerebellar Cortex in the Naked Mole-Rat (Heterocephalus glaber)

et al. 2011

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Despite the apparent uniformity in cellular composition of the adult mammalian cerebellar cortex, it is actually highly compartmentalized into transverse zones and within each zone further subdivided into a reproducible array of parasagittal stripes. This basic cerebellar architecture is highly conserved in birds and mammals. However, different species have very different cerebellar morphologies, and it is unclear if cerebellar architecture reflects taxonomic relations or ecological niches. To explore this, we have examined the cerebellum of the naked mole-rat Heterocephalus glaber, a burrowing rodent with adaptations to a subterranean life that include only a rudimentary visual system. The cerebellum of H. glaber resembles that of other rodents with the remarkable exception that cerebellar regions that are prominent in the handling of visual information (the central zone, nodular zone, and dorsal paraflocculus) are greatly reduced or absent. In addition, there is a notable increase in size in the posterior zone, consistent with an expanded role for the trigeminal somatosensory system. These data suggest that cerebellar architecture may be substantially modified to serve a particular ecological niche.

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Constitutive expression of the 25-kDa heat shock protein Hsp25 reveals novel parasagittal bands of Purkinje cells in the adult mouse cerebellar cortex

Cited by 143 publications

References 68 publications

Dysbindin, a Novel Coiled-coil-containing Protein That Interacts with the Dystrobrevins in Muscle and Brain

Dysbindin, a Novel Coiled-coil-containing Protein That Interacts with the Dystrobrevins in Muscle and Brain

Zebrin-Immunopositive and -Immunonegative Stripe Pairs Represent Functional Units in the Pigeon Vestibulocerebellum

Compartmentation of the Cerebellar Cortex in the Naked Mole-Rat (Heterocephalus glaber)

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