Constitutive Expression of Pea Defense Gene DRR206 Confers Resistance to Blackleg (<i>Leptosphaeria maculans</i>) Disease in Transgenic Canola (<i>Brassica napus</i>)

Wang, Yaping; Nowak, Goska; Culley, David E.; Hadwiger, Lee A.; Fristensky, Brian

doi:10.1094/mpmi.1999.12.5.410

Cited by 117 publications

(58 citation statements)

References 29 publications

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“…Half reduction in the size of the lesions caused by Magnaporthe grisea was achieved via transformation with wasabi defensin gene in rice (14). enhanced resistance to early blight (Alternaria solani) in tomato plants expressing plant defensin gene from Mirabilis jalapa was also observed (22).Similar results were achieved in canola (Brassica napus) by the use of the pea defense gene DRR206 towards blackleg (leptosphaeria maculans) disease (32).…”

Section: Molecular Analysis Of Tsupporting

confidence: 57%

Constitutive Expression of a Radish Defensin GeneRs-AFP2in Tomato Increases the Resisstance to Fungal Pathogens

Kostov

Christova

Slavov

et al. 2009

Biotechnology & Biotechnological Equipment

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Section: Molecular Analysis Of Tsupporting

confidence: 57%

Constitutive Expression of a Radish Defensin GeneRs-AFP2in Tomato Increases the Resisstance to Fungal Pathogens

Kostov

Christova

Slavov

et al. 2009

Biotechnology & Biotechnological Equipment

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“…This DIR superfamily contains a number of proteins whose expression levels are induced during plant-disease response and lignification. According to the classification of Ralph et al (26), the Pdh1-encoded protein belongs to DIR protein group a, which includes DIR(-like) proteins from Forsythia × intermedia (27) and pea (28) (Fig. S5).…”

Section: Resultsmentioning

confidence: 99%

Molecular basis of a shattering resistance boosting global dissemination of soybean

Funatsuki

Suzuki

Hirose

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

178

223

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Pod dehiscence (shattering) is essential for the propagation of wild plant species bearing seeds in pods but is a major cause of yield loss in legume and crucifer crops. Although natural genetic variation in pod dehiscence has been, and will be, useful for plant breeding, little is known about the molecular genetic basis of shattering resistance in crops. Therefore, we performed map-based cloning to unveil a major quantitative trait locus (QTL) controlling pod dehiscence in soybean. Fine mapping and complementation testing revealed that the QTL encodes a dirigent-like protein, designated as Pdh1. The gene for the shattering-resistant genotype, pdh1, was defective, having a premature stop codon. The functional gene, Pdh1, was highly expressed in the lignin-rich inner sclerenchyma of pod walls, especially at the stage of initiation in lignin deposition. Comparisons of near-isogenic lines indicated that Pdh1 promotes pod dehiscence by increasing the torsion of dried pod walls, which serves as a driving force for pod dehiscence under low humidity. A survey of soybean germplasm revealed that pdh1 was frequently detected in landraces from semiarid regions and has been extensively used for breeding in North America, the world's leading soybean producer. These findings point to a new mechanism for pod dehiscence involving the dirigent protein family and suggest that pdh1 has played a crucial role in the global expansion of soybean cultivation. Furthermore, the orthologs of pdh1, or genes with the same role, will possibly be useful for crop improvement.

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“…(Fig. 3A) belongs to the "disease resistance response protein 206 (DRR206)," which plays an important role in the plant defense against fungi (42). The Lys-acetylated peptide VFD ac KDQNGFISAAELR (Fig.…”

Section: Resultsmentioning

confidence: 99%

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

Melo‐Braga

Verano‐Braga

León

et al. 2012

Molecular & Cellular Proteomics

110

112

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Grapevine (Vitis vinifera) is an economically important fruit crop that is subject to many types of insect and pathogen attack. To better elucidate the plant response to Lobesia botrana pathogen infection, we initiated a global comparative proteomic study monitoring steady-state protein expression as well as changes in N-glycosylation, phosphorylation, and Lys-acetylation in control and infected mesocarp and exocarp from V. vinifera cv Italia. A multi-parallel, large-scale proteomic approach employing iTRAQ labeling prior to three peptide enrichment techniques followed by tandem mass spectrometry led to the identification of a total of 3059 proteins, 1135 phosphorylation sites, 323 N-linked glycosylation sites and 138 Lysacetylation sites. Of these, we could identify changes in abundance of 899 proteins. The occupancy of 110 phosphorylation sites, 10 N-glycosylation sites and 20 Lysacetylation sites differentially changed during L. botrana infection. Sequence consensus analysis for phosphorylation sites showed eight significant motifs, two of which containing up-regulated phosphopeptides (X-G-S-X and S-X-X-D) and two containing down-regulated phosphopeptides (R-X-X-S and S-D-X-E) in response to

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Constitutive Expression of Pea Defense Gene DRR206 Confers Resistance to Blackleg (Leptosphaeria maculans) Disease in Transgenic Canola (Brassica napus)

Cited by 117 publications

References 29 publications

Constitutive Expression of a Radish Defensin GeneRs-AFP2in Tomato Increases the Resisstance to Fungal Pathogens

Constitutive Expression of a Radish Defensin GeneRs-AFP2in Tomato Increases the Resisstance to Fungal Pathogens

Molecular basis of a shattering resistance boosting global dissemination of soybean

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

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