Constitutive expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and glucose and ABA insensitivity in mustard transgenic plants

Ahmed, Israr; Yadav, Deepanker; Shukla, Pawan; Vineeth, T. V.; Sharma, Parbodh Chander; Kirti, Pulugurtha Bharadwaja

doi:10.1016/j.plantsci.2017.09.010

Cited by 30 publications

(15 citation statements)

References 117 publications

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“…Biophysical analysis revealed that the stress tolerance of 35S:OsAnn5 transgenics are correlated with enhanced osmolyte (proline) accumulation, reduced peroxidation and chlorophyll content in the cells. This kind of correlation was also observed with other annexin transgenics (Ahmed et al, 2017;Huh et al, 2010). DAB staining assay results supported the MDA quantification results for 35S:OsAnn5 transgenics under salt stress.…”

Section: Aba-independent Expression Of Osann5 Under Salt Stresssupporting

confidence: 73%

“…overexpressing transgenic plants of mustard were also insensitive to ABA during the seed germination stage. Interestingly, gene expression studies in the AnnBj2 transgenic mustard plants revealed increased transcript levels of ABA catabolic gene CYP707A2 (Ahmed et al, 2017). In line with these results, OsAnn3 knock down transgenics showed ABA sensitivity at seed germination stage (Shen et al, 2017) suggesting that annexins may enhance seed germination by promoting the degradation of ABA.…”

Section: Osann5 Expression Is Possibly Independent Of Aba Under Salt mentioning

confidence: 52%

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Ectopic overexpression of abiotic stress-induced rice annexin,OsAnn5potentiates tolerance to abiotic stresses

Boyidi

Vikas

Botta

et al. 2019

Preprint

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The current study on putative rice annexin OsAnn5 was tried to know its functional role in the abiotic stress tolerance. For this an in silico analysis of its protein sequence and upstream region was carried out. This results in identification of several probable potential sites for posttranslational modifications and cis-elements respectively. We have studied the effect of OsAnn5 in the amelioration of abiotic stress tolerance through heterologous expression in transgenic tobacco and E.coli. It is observed that OsAnn5 over expression leads to enhanced tolerance to abiotic stress through efficient scavenging of the ROS and balanced expression of SOD and CAT antioxidant enzymes in both the systems, under stress treatments. Fluorescent signal for transiently expressed EGFP:OsANN5 fusion protein was localized in the peripheral region of the onion epidermal cells under salt stress treatment. Expression analysis of OsAnn5 under ABA synthesis inhibitor, fluridone and salinity stress revealed that OsAnn5 appears to act through an ABA-independent pathway under salt stress and in support to this 35S:OsAnn5 transgenics seedlings exhibited less sensitivity to externally applied ABA.

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Section: Aba-independent Expression Of Osann5 Under Salt Stresssupporting

confidence: 73%

Section: Osann5 Expression Is Possibly Independent Of Aba Under Salt mentioning

confidence: 52%

Ectopic overexpression of abiotic stress-induced rice annexin,OsAnn5potentiates tolerance to abiotic stresses

Boyidi

Vikas

Botta

et al. 2019

Preprint

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“…Overexpression of cotton annexin gene AnnGh3 increased trichome density and length in Arabidopsis leaf 36 . Overexpression of Brassica juncea annexin AnnBj2 increased salt tolerance and abscisic acid (ABA) insensitivity in transgenic plants 37,38 . Ectopic expression of B. juncea annexin gene BjAnn1 in tobacco and cotton enhanced tolerance to various abiotic stresses and fungal pathogen [39][40][41] .…”

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confidence: 99%

Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response

Liao

Xie

et al. 2020

Sci Rep

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Annexins (Ann) are a multigene, evolutionarily conserved family of calcium-dependent and phospholipid-binding proteins that play important roles in plant development and stress resistance. However, a systematic comprehensive analysis of ANN genes of Brassicaceae species (Brassica rapa, Brassica oleracea, and Brassica napus) has not yet been reported. In this study, we identified 13, 12, and 26 ANN genes in B. rapa, B. oleracea, and B. napus, respectively. About half of these genes were clustered on various chromosomes. Molecular evolutionary analysis showed that the ANN genes were highly conserved in Brassicaceae species. Transcriptome analysis showed that different group ANN members exhibited varied expression patterns in different tissues and under different (abiotic stress and hormones) treatments. Meanwhile, same group members from Arabidopsis thaliana, B. rapa, B. oleracea, and B. napus demonstrated conserved expression patterns in different tissues. The weighted gene coexpression network analysis (WGcnA) showed that BnaANN genes were induced by methyl jasmonate (MeJA) treatment and played important roles in jasmonate (JA) signaling and multiple stress response in B. napus.Annexins (ANN) are a multigene, evolutionarily conserved family of calcium (Ca 2+ )-dependent and phospholipid-binding proteins present in plants, animals, and microorganisms 1,2 . ANN contain the characteristic annexin repeat and they regulate membrane dynamics, mediate Ca 2+ sensing and signaling, link Ca 2+ dynamics to cytoskeletal responses, and mediate immune or stress responses and signaling during plant growth and development 1,3 . A typical ANN contains four annexin repeats at the C-terminal region and a highly variable N-terminal region. Each annexin repeat usually contains a characteristic type II motif for Ca 2+ binding 1,3 . The variable N-terminal region interacts with other proteins and is responsible for the functional diversity of ANN 4 .Recent studies have identified the ANN gene family in Arabidopsis thaliana (8 genes), Brassica rapa (13), Solanum lycopersicum (9), Solanum tuberosum (9), Oryza sativa (10), Triticum aestivum (25), Gossypium raimondii (14), Arachis hypogaea L. (8), Hordeum vulgare (11), Medicago truncatula (10), Populus trichocarpa (12), Vitis vinifera (14), Carica papaya (12), Glycine max (22), Cochliobolus sativus (11), Sorghum bicolor (10), Zea mays (12), Brachypodium distachyon (11), Selaginella mollendorffii (5), and Physcomitrella patens (7) via genome-wide analysis 2,5-11 .Studies have shown that ANN gene family plays a significant role in plant development and plant protection during both abiotic and biotic stresses 1,3,12,13 . In Arabidopsis, two ANN genes (AtANN1 and AtANN4) were regulated by abiotic stress, negatively regulated plant tolerance to drought, salinity, and heat stress, while AtANN8 was open Scientific RepoRtS | (2020) 10:4295 | https://doi.org/10.1038/s41598-020-59953-w www.nature.com/scientificreports www.nature.com/scientificreports/ positive regulated the plant abiotic tolerance [...

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“…1B) indicated impairment of the cell membrane systems during early imbibition of seeds, and the decrease of this value indicated subsequent repair of the damaged membrane as seed germination progressed. Given the essential function of annexins in PM resealing in animal cells (Andrews et al , 2014; Draeger et al , 2014), the plant annexins were proposed as possible components involved in the PM repair (Schapire et al ., 2008; Draeger et al , 2011) as cytosolic, peripheral, or integral membrane proteins (Talukdar et al , 2009). However, how the repair of the damaged membrane is achieved remains unclear, and it is uncertain whether annexins benefit membrane recovery during maize germination.…”

Section: Discussionmentioning

confidence: 99%

Maize annexin genesZmANN33andZmANN35encode proteins that function in cell membrane recovery during seed germination

Gao

Guo

et al. 2019

Journal of Experimental Botany

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Constitutive expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and glucose and ABA insensitivity in mustard transgenic plants

Cited by 30 publications

References 117 publications

Ectopic overexpression of abiotic stress-induced rice annexin,OsAnn5potentiates tolerance to abiotic stresses

Ectopic overexpression of abiotic stress-induced rice annexin,OsAnn5potentiates tolerance to abiotic stresses

Comprehensive analyses of the annexin (ANN) gene family in Brassica rapa, Brassica oleracea and Brassica napus reveals their roles in stress response

Maize annexin genesZmANN33andZmANN35encode proteins that function in cell membrane recovery during seed germination

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