Constitutive expression of an endoplasmic reticulum small heat shock protein alleviates endoplasmic reticulum stress in transgenic tomato

Zhao, Chun‐Mei; Shono, Masayuki; Sun, Aijun; Yi, Shuying; Li, Minghui; Liu, Jian

doi:10.1016/j.jplph.2006.06.004

Cited by 36 publications

(31 citation statements)

References 25 publications

(29 reference statements)

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“…1D). To our knowledge, such an efficient accumulation has not been reported previously in comparable studies (Zhao et al, 2007;Jiang et al, 2009;Kim et al, 2011). Besides having the right size, the transgene product retained the ability of the native protein to form oligomeric complexes under nondissociating conditions.…”

Section: Discussionmentioning

confidence: 56%

“…Likewise, the role of sHSPs in mechanisms of thermotolerance has not yet been established. Reverse genetic approaches have led to inconsistent results in Arabidopsis (Arabidopsis thaliana) and other herbaceous species, ranging from variable protection (Malik et al, 1999;Sanmiya et al, 2004;Zhao et al, 2007;Jiang et al, 2009) to no detectable effects at all (Härndahl et al, 1999;Sun et al, 2001).…”

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confidence: 99%

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Plantation Forestry under Global Warming: Hybrid Poplars with Improved Thermotolerance Provide New Insights on the in Vivo Function of Small Heat Shock Protein Chaperones

et al. 2013

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Climate-driven heat stress is a key factor affecting forest plantation yields. While its effects are expected to worsen during this century, breeding more tolerant genotypes has proven elusive. We report here a substantial and durable increase in the thermotolerance of hybrid poplar (Populus tremula 3 Populus alba) through overexpression of a major small heat shock protein (sHSP) with convenient features. Experimental evidence was obtained linking protective effects in the transgenic events with the unique chaperone activity of sHSPs. In addition, significant positive correlations were observed between phenotype strength and heterologous sHSP accumulation. The remarkable baseline levels of transgene product (up to 1.8% of total leaf protein) have not been reported in analogous studies with herbaceous species. As judged by protein analyses, such an accumulation is not matched either by endogenous sHSPs in both heat-stressed poplar plants and field-grown adult trees. Quantitative real timepolymerase chain reaction analyses supported these observations and allowed us to identify the poplar members most responsive to heat stress. Interestingly, sHSP overaccumulation was not associated with pleiotropic effects that might decrease yields. The poplar lines developed here also outperformed controls under in vitro and ex vitro culture conditions (callus biomass, shoot production, and ex vitro survival), even in the absence of thermal stress. These results reinforce the feasibility of improving valuable genotypes for plantation forestry, a field where in vitro recalcitrance, long breeding cycles, and other practical factors constrain conventional genetic approaches. They also provide new insights into the biological functions of the least understood family of heat shock protein chaperones.

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Section: Discussionmentioning

confidence: 56%

mentioning

confidence: 99%

Plantation Forestry under Global Warming: Hybrid Poplars with Improved Thermotolerance Provide New Insights on the in Vivo Function of Small Heat Shock Protein Chaperones

et al. 2013

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“…Some data indicated that particular ER-located sHSPs in plants may have play the molecular chaperone functions stabilising proteins under stress conditions. Genetically engineered tomato carrying LeHSP21.5 displayed improved tolerance to tunicamycin-ER stress inducer (Zhao et al 2007). …”

Section: Heat Shock Proteinsmentioning

confidence: 99%

Tomato tolerance to abiotic stress: a review of most often engineered target sequences

Gerszberg

Hnatuszko-Konka

2017

Plant Growth Regul

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“…Most ER-residing molecular chaperones are significantly induced by tunicamycin and DTT (Martínez and Chrispeels, 2003;Kamauchi et al, 2005), but the ER-located small heat-shock protein (hereafter ER-sHSP) is an exception (Zhao et al, 2007). ER-sHSP has the functional activity of a molecular chaperone (Zhao et al, 2007;Mamedov and Shono, 2008) and its overexpression can alleviate ER stress caused by tunicamycin and DTT exposure (Zhao et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…ER-sHSP has the functional activity of a molecular chaperone (Zhao et al, 2007;Mamedov and Shono, 2008) and its overexpression can alleviate ER stress caused by tunicamycin and DTT exposure (Zhao et al, 2007). The aim of this study was to determine whether the constitutive expression of ER-sHSP would improve the abiotic tolerance of plants, as does overexpression of BiP (Alvim et al, 2001;Valente et al, 2009) and an increased HSP90 level (Ling et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Enhanced salt tolerance in tomato plants constitutively expressing heat-shock protein in the endoplasmic reticulum

Liu

Yang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and activates the unfolded protein response (UPR) signaling pathway. The UPR signaling pathway is associated with plant responses to adverse environmental conditions. Thus, changes in the UPR signaling pathway might affect plant abiotic tolerance. Here, the role of ER small heat-shock protein (ER-sHSP) in improving plant resistance to salt stress was explored. Under salt stress conditions, ER-sHSP transgenic plants were found to have more vigorous roots, maintain a higher relative water content, absorb less Na + , accumulate more osmolytes and Ca 2+ , and sustain less damage to the photosystem, compared to wild-type non-transgenic plants. Furthermore, we found that the constitutive expression of ER-sHSP under salt stress depressed the expression of other ER molecular chaperones. These results indicate that the constitutive expression of ER-sHSP enhanced salinity tolerance of tomato plants significantly, and alleviated the ER stress caused by the salt stress in plant cells.

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Constitutive expression of an endoplasmic reticulum small heat shock protein alleviates endoplasmic reticulum stress in transgenic tomato

Cited by 36 publications

References 25 publications

Plantation Forestry under Global Warming: Hybrid Poplars with Improved Thermotolerance Provide New Insights on the in Vivo Function of Small Heat Shock Protein Chaperones

Plantation Forestry under Global Warming: Hybrid Poplars with Improved Thermotolerance Provide New Insights on the in Vivo Function of Small Heat Shock Protein Chaperones

Tomato tolerance to abiotic stress: a review of most often engineered target sequences

Enhanced salt tolerance in tomato plants constitutively expressing heat-shock protein in the endoplasmic reticulum

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