Constitutive Expression of a Novel-Type Ammonium Transporter OsAMT2 in Rice Plants

Suenaga, A.; Moriya, Kensuke; Sonoda, Yutaka; Ikeda, Akira; Wirén, Nicolaus von; Hayakawa, Toshihiko; Yamaguchi, Junji; Yamaya, Tomoyuki

doi:10.1093/pcp/pcg017

Cited by 171 publications

(120 citation statements)

References 23 publications

(31 reference statements)

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“…From this point of view, LjAMT2;2, with its two introns, fits well into the AMT2 subfamily. This picture is also confirmed by the phylogenetic analysis that placed LjAMT2;2 in the AMT2 subgroup 4 (Suenaga et al, 2003) of O. sativa and P. trichocarpa (Couturier et al, 2007). Interestingly, transcripts from this subgroup are rare or not detected at all in several species (Suenaga et al, 2003;Couturier et al, 2007).…”

Section: Discussionsupporting

confidence: 57%

“…This picture is also confirmed by the phylogenetic analysis that placed LjAMT2;2 in the AMT2 subgroup 4 (Suenaga et al, 2003) of O. sativa and P. trichocarpa (Couturier et al, 2007). Interestingly, transcripts from this subgroup are rare or not detected at all in several species (Suenaga et al, 2003;Couturier et al, 2007). EST sequences have so far only been reported for two members: PtrAMT4;2 in floral buds and PtrAMT4;5 in poplar cultured cells (Couturier et al, 2007).…”

Section: Discussionmentioning

confidence: 58%

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A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi

et al. 2009

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In mycorrhizal associations, the fungal partner assists its plant host by providing nitrogen (N) in addition to phosphate. Arbuscular mycorrhizal (AM) fungi have access to inorganic or organic forms of N and translocate them via arginine from the extra-to the intraradical mycelium, where the N is transferred to the plant without any carbon skeleton. However, the molecular form in which N is transferred, as well as the involved mechanisms, is still under debate. NH 4 + seems to be the preferential transferred molecule, but no plant ammonium transporter (AMT) has been identified so far. Here, we offer evidence of a plant AMT that is involved in N uptake during mycorrhiza symbiosis. The gene LjAMT2;2, which has been shown to be the highest up-regulated gene in a transcriptomic analysis of Lotus japonicus roots upon colonization with Gigaspora margarita, has been characterized as a high-affinity AMT belonging to the AMT2 subfamily. It is exclusively expressed in the mycorrhizal roots, but not in the nodules, and transcripts have preferentially been located in the arbusculated cells. Yeast (Saccharomyces cerevisiae) mutant complementation has confirmed its functionality and revealed its dependency on acidic pH. The transport experiments using Xenopus laevis oocytes indicated that, unlike other plant AMTs, LjAMT2;2 transports NH 3 instead of NH 4 + . Our results suggest that the transporter binds charged ammonium in the apoplastic interfacial compartment and releases the uncharged NH 3 into the plant cytoplasm. The implications of such a finding are discussed in the context of AM functioning and plant phosphorus uptake.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 58%

A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi

et al. 2009

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“…Members of the AMT1 and AMT2 subfamilies differ in their biochemical and sequence characteristics with the latter being more closely related to bacterial AMT transporters (Sohlenkamp et al 2000). Genome sequencing has revealed three AMT1 genes in Oriza sativa , five in Arabidopsis thaliana and eight in Populus trichocarpa (Couturier et al 2007); whereas one, six and seven AMT2 genes were identified in A. thaliana (Sohlenkamp et al 2000) P. trichocarpa (Couturier et al 2007) and O. sativa (Suenaga et al 2003), respectively.…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus

et al. 2008

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Plant ammonium transporters of the AMT1 family are involved in N-uptake from the soil and ammonium transport, and recycling within the plant. Although AMT1 genes are known to be expressed in nitrogen-fixing nodules of legumes, their precise roles in this specialized organ remain unknown. We have taken a reverse-genetic approach to decipher the physiological role of LjAMT1;1 in Lotus japonicus nodules. LjAMT1;1 is normally expressed in both the infected zone and the vascular tissue of Lotus nodules. Inhibition of LjAMT1;1 gene expression, using an antisense gene construct driven by a leghemoglobin promoter resulted in a substantial reduction of LjAMT1;1 transcript in the infected tissue but not the vascular bundles of transgenic plants. As a result, the nitrogen-fixing activity of nodules was partially impaired and nodule number increased compared to control plants. Expression of LjAMT1;1-GFP fusion protein in plant cells indicated a plasma-membrane location for the LjAMT1;1 protein.Taken together, the results are consistent with a role of LjAMT1;1 in retaining ammonium derived from symbiotic nitrogen fixation in plant cells prior to its assimilation.

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“…AMT1 gene family was first identified from Saccharomyces and Arabidopsis (Gazzarrini et al 1999), subsequently cloned from Oryza sativa (Suenaga et al 2003), Medicago truncatula (Straub et al 2014), Lotus japonicas (Salvemini et al 2001), Brassica napus (Pearson et al 2002), Lycopersicon esculentum (von Wiren et al 2000), Populus trichocarpa (Couturier et al 2007), Sorghum bicolor (Koegel et al 2013) etc. Among woody plants constitutive organ-specific AMT1 transcription was detected only in Populus (Couturier et al 2007), Citrus (Camanes et al 2007) and Pyrus (Li et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Influence of different host associations on glutamine synthetase activity and ammonium transporter in Santalum album L.

Deepa

Yusuf

2016

Physiol Mol Biol Plants

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The present study was aimed at understanding the role of different hosts in ammonium transporter1;2 expressions and glutamine synthetase(GS) activity and their effects on the growth parameters in the sandal. Sandal plant associated with leguminous host expressed better growth parameters. GS activity of leguminous hosts alone and in host associated sandals was analyzed using GS transferase assay. Highest GS activity was expressed in Mimosa pudica-sandal association compared to other leguminous and non-leguminous host associations. The association of N 2 fixing host with sandal enhanced C and N levels in order to maintain the C/N value. The role of ammonium transporters in N nutrition of sandal-host association was elucidated by cloning AMT1;2 from the leaves, haustoria and roots of host associated sandal and quantifying the relative expression by the 2 ÀDDC T method. SaAMT1;2 was strongly up-regulated in leaves, roots and haustoria of leguminous host associated sandal compared to non-leguminous host associations. The relative increase in SaAMT1;2 expressions and up-regulated GS activity positively affected the growth parameters in sandal when associated with leguminous hosts.

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Constitutive Expression of a Novel-Type Ammonium Transporter OsAMT2 in Rice Plants

Cited by 171 publications

References 23 publications

A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi

A Mycorrhizal-Specific Ammonium Transporter from Lotus japonicus Acquires Nitrogen Released by Arbuscular Mycorrhizal Fungi

Tissue-specific down-regulation of LjAMT1;1 compromises nodule function and enhances nodulation in Lotus japonicus

Influence of different host associations on glutamine synthetase activity and ammonium transporter in Santalum album L.

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