Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Alexandersson, Erik; Becker, John V.W.; Jacobson, Dan; Nguema-Ona, Éric; Steyn, Cobus; Denby, Katherine J.; Vivier, Melané A.

doi:10.1186/1756-0500-4-493

Cited by 31 publications

(39 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PGIPs are plant cell-wall proteins that specifically inhibit fungal endopolygalacturonases (PGs) that contribute to the aggressive decomposition of susceptible plant tissues. 72 Induction of PGIP gene has been reported in Norton grape, 66 and reduction in PGIP activity was observed in Cabernet Sauvignon. 9 Vicilin-7S-like antimicrobial peptides that belong to the cupin-2 superfamily 73 protein was upregulated 6-fold (Figure 7B), while the transcript level increased 11-fold between the GH and PS stages (Figure 8V) confirming an increase in protein expression pattern.…”

Section: Resultsmentioning

confidence: 97%

iTRAQ-Based Quantitative Proteomics of Developing and Ripening Muscadine Grape Berry

et al. 2013

View full text Add to dashboard Cite

Grapes are among the widely cultivated fruit crops in the world. Grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. Muscadine grapes are widely cultivated in the southern United States for fresh fruit and wine. To date, changes in the metabolites composition of muscadine grapes have been well documented; however, the molecular changes during berry development and ripening are not fully known. The aim of this study was to investigate changes in the berry proteome during ripening in muscadine grape cv. Noble. Isobaric tags for relative and absolute quantification (iTRAQ) MS/MS was used to detect statistically significant changes in the berry proteome. A total of 674 proteins were detected, and 76 were differentially expressed across four time points in muscadine berry. Proteins obtained were further analyzed to provide information about its potential functions during ripening. Several proteins involved in abiotic and biotic stimuli and sucrose and hexose metabolism were upregulated during berry ripening. Quantitative real-time PCR analysis validated the protein expression results for nine proteins. Identification of vicilin-like antimicrobial peptides indicates additional disease tolerance proteins are present in muscadines for berry protection during ripening. The results provide new information for characterization and understanding muscadine berry proteome and grape ripening.

show abstract

Section: Resultsmentioning

confidence: 97%

iTRAQ-Based Quantitative Proteomics of Developing and Ripening Muscadine Grape Berry

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In agreement with this interpretation, we observed a higher average molecular mass for pectins isolated from pgx1 mutant walls and a lower average molecular mass for pectins isolated from PGX1 AT and PGX1 OE walls ( Figure 8B), indicating that PGX1 cleaves HG (Atkinson et al, 2002;Posé et al, 2013), possibly reducing the capacity of the pectin network to cross-link and enabling wall expansion. At this point, we do not know how PGX1 activity is limited in cell walls to prevent it from aberrantly degrading pectin, but it is possible that it is regulated by limited mobility in intact walls and/or binding to PG inhibitor proteins (Alexandersson et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

POLYGALACTURONASE INVOLVED IN EXPANSION1 Functions in Cell Elongation and Flower Development in Arabidopsis

2014

View full text Add to dashboard Cite

Pectins are acidic carbohydrates that comprise a significant fraction of the primary walls of eudicotyledonous plant cells. They influence wall porosity and extensibility, thus controlling cell and organ growth during plant development. The regulated degradation of pectins is required for many cell separation events in plants, but the role of pectin degradation in cell expansion is poorly defined. Using an activation tag screen designed to isolate genes involved in wall expansion, we identified a gene encoding a putative polygalacturonase that, when overexpressed, resulted in enhanced hypocotyl elongation in etiolated Arabidopsis thaliana seedlings. We named this gene POLYGALACTURONASE INVOLVED IN EXPANSION1 (PGX1). Plants lacking PGX1 display reduced hypocotyl elongation that is complemented by transgenic PGX1 expression. PGX1 is expressed in expanding tissues throughout development, including seedlings, roots, leaves, and flowers. PGX1-GFP (green fluorescent protein) localizes to the apoplast, and heterologously expressed PGX1 displays in vitro polygalacturonase activity, supporting a function for this protein in apoplastic pectin degradation. Plants either overexpressing or lacking PGX1 display alterations in total polygalacturonase activity, pectin molecular mass, and wall composition and also display higher proportions of flowers with extra petals, suggesting PGX1's involvement in floral organ patterning. These results reveal new roles for polygalacturonases in plant development.

show abstract

“…Several grapevine PR genes have been characterised and these include signalling PR1 (Wielgoss and Kortekamp 2006), hydrolytic enzymes PR2 (β-1,3 glucanases), PR3, PR4 (chitinases) and chitinase class IV (CHIT IV), PR 5 (osmotin) (Jacobs et al 1999), antimicrobial PR6 (serine proteases inhibitor) and polygalacturonase-inhibiting proteins (PGIP) (De Lorenzo and Ferrari 2002;Joubert et al 2006;Alexandersson et al 2011, Nguema-Ona et al 2013. Expression of PR proteins is selective and depends on the infecting pathogen or the elicitor (Glazebrook 2005).…”

Section: Introductionmentioning

confidence: 99%

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

et al. 2016

View full text Add to dashboard Cite

Cell suspension cultures of Vitis vinifera cv. Dauphine berries were used to study the response to the vascular pathogen, Eutypa lata, in comparison with a biological control agent, Trichoderma atroviride, that was previously shown to be effective in pruning wound protection. The expression of genes coding for enzymes of the phenylpropanoid pathway and pathogenesis-related (PR) proteins was profiled over a 48-h period using quantitative reverse transcriptase PCR. The cell cultures responded to elicitors of both fungi with a hypersensitive-like response that lead to a decrease in cell viability. Similar genes were triggered by both the pathogen and biocontrol agent, but the timing patterns and magnitude of expression was dependent on the specific fungal elicitor. Culture filtrates of both fungi caused upregulation of phenylalanine ammonia-lyase (PAL), 4-coumaroyl Co-A ligase (CCo-A) and stilbene synthase (STS), and a downregulation of chalcone synthase (CHS) genes. The pathogen filtrate caused a biphasic pattern in the upregulation of PAL and STS genes which was not observed in cells treated with filtrates of the biocontrol agent. Analytical assays showed significantly higher total phenolic content and chitinolytic enzyme activity in the cell cultures treated with the T. atroviride filtrate compared to the pathogen filtrate. These results corresponded well to the higher expression of PAL and chitinase class IV genes. The response of the cell cultures to T. atroviride filtrate provides support for the notion that the wound protection by the biocontrol agent at least partially relies on the induction of grapevine resistance mechanisms.

show abstract

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Cited by 31 publications

References 69 publications

iTRAQ-Based Quantitative Proteomics of Developing and Ripening Muscadine Grape Berry

iTRAQ-Based Quantitative Proteomics of Developing and Ripening Muscadine Grape Berry

POLYGALACTURONASE INVOLVED IN EXPANSION1 Functions in Cell Elongation and Flower Development in Arabidopsis

Response of Vitis vinifera cell cultures to Eutypa lata and Trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes

Contact Info

Product

Resources

About