Constituintes químicos das cascas do caule de Cenostigma macrophyllum: ocorrência de colesterol

Abstract

Recebido em 28/9/06; aceito em 27/4/07; publicado na web em 25/10/07 CHEMICAL CONSTITUENTS FROM BARK OF Cenostigma macrophyllum: CHOLESTEROL OCCURRENCE. Phytochemical investigation of the bark of Cenostigma macrophyllum (Leguminosae-Caesapinioideae) resulted in the isolation and identification of valoneic acid dilactone, ellagic acid, lupeol, alkyl ferulate, four free sterols (cholesterol, campesterol, stigmasterol and sitosterol), a mixture of sitosteryl ester derivatives of fatty acids, sitosterol-3-O-β-D-glucopyranoside, stigmasterol-3-O-β-D-glucopyranoside and saturated and unsaturated fatty acids. The structures of the isolated compounds were identified by 1 H and 13 C NMR spectral analysis and comparison with literature data. The mixtures of 3-β-hydroxysterols and fatty acids were analysed by GC/MS.

Evaluation of anti-inflammatory, antinociceptive and biological activities of Cenostigma macrophyllum standardized extracts and determination and quantification of the main metabolites

“…The presented chromatographic method was successfully applied in the simultaneous determination of 1-5 in the extracts MBCM and MLCM (Table 5). Ellagic acid (3) is the compound present in major concentrations in both extracts and the bioavonoids agathisavone (4) and amentoavone (5) were detected only on leaves which is in accordance with the literature, since 4 and 5 were isolated only from the leaves of C. macrophyllum, 7,11 whereas ellagic acid was isolated from both leaves and stem bark 3,7 and, this is the rst report of gallic acid and methyl gallate in the stem bark of this species. In the previous work 11 from stem bark from a specimen collected in different habitat was isolated bergenin as the main compound of this species.…”

“…1,2 The decoction or infusion of stem barks, leaves and owers of this species are used in folk medicine for the treatment of stomach and intestinal diseases. 3 Cenostigma macrophyllum of the "Caatinga" Brazilian biome was classied until recently as another specie (C. gardnerianum Tul.) but they were considered as synonyms aer the morphological variation analysis.…”

“…[8][9][10] Phytochemical studies with C. macrophyllum have led to the isolation and identication of avonoids, biavonoids, benzoic acid derivatives, peptide derivatives, triterpenoids, steroids, vitamin A and E, fatty acids, coumarin and volatile esters. 3,11,12 However, there are no reports in the literature on the development of a validated chromatographic method for the quantication of active principles present in this species. Thus, in the present study, a HPLC-DAD method was developed to simultaneously detect and quantify ve bioactive compounds ( Fig.…”

Avaliação do potencial alelopático de substâncias isoladas em sementes de araticum (Annona crassiflora)

“…São os esteroides glicosilados: estigmasterol glicosilado (ES5) e sitosterol glicosilado (ES6). (Hung & Yen, 2001;Falcão, 2003;Rocha & Silva et al, 2007).…”

Gastroprotective effect of lupeol on ethanol-induced gastric damage and the underlying mechanism

“…Lupeol was isolated from the stem bark of Cenostigma macrophyllum (Leguminosae-Caesapinioideae) as per procedures described earlier (Rocha e Silva et al 2007). Absolute ethanol (EtOH) was obtained from BDH, UK; N x -Nitro-l-arginine methyl ester (L-NAME), N-acetylcysteine, clonidine, diazoxide, glibenclamide, glutathione, thiobarbituric acid, yohimbine from Sigma Chemical Company, St. Louis.…”