“…This species occurs generally on plants in warm temperate and tropical regions 26,27 . There are reports of B. bicolor being isolated from plant leaf spot and root rot affected St. Augustine grass ( Stenotaphrum secundatum ), 28 signalgrass ( Brachiaria decumbens ), 29 hemp palm ( Trachycarpus fortunei ) 30 and the rubber tree ( Ficus elastica ) 31 . In 2017, although the strain CPC28811 of B. bicolor was first reported from new host goosegrass in Thailand, no descriptions about its morphological characteristics and disease symptoms were given 27 .…”
Section: Resultsmentioning
confidence: 99%
“…The reaction system with a 25 μL volume contained 12.5 μL 2 × Hieff PCR Master Mix with Dye (Yeasen Biotech Co., Ltd, Shanghai, China), 1 μL of each 10 μM primer, 1 μL of template DNA and 9.5 μL of sterilized distilled water. The PCR reaction programs were as follows: an initial denaturing step at 94 °C for 5 min, followed by 30…”
Section: Molecular Identification Of the Synjc-2-2 Strainmentioning
confidence: 99%
“…After 20-μL conidial suspensions of 5 × 10 5 were dripped onto a glass slide with a grooved surface and dried in air on a superclean bench, 20 μL of PDB liquid media (potato 200 g L −1 , sucrose 20 g L −1 ) with different pH (1-12) were added onto the grooved glass slides and incubated for 6 h at 28 °C and 100% relative humidity in darkness, or the dried conidia on grooved glass slides were directly placed in different relative humidity conditions (69%, 75%, 85%, 90%, 94%, 98% and 100%) and incubated for 12 h at 28 °C in darkness. To evaluate the effect of temperature and light on conidial germination, 20 μL of conidial suspensions of 5 × 10 5 were dripped onto the grooved glass slides and incubated for 6 h at various temperatures (15,20,25,30,35,40,45,49, 50 and 51 °C) under dark or at 28 °C under various illuminations (dark, fluorescence light, UV light and black light). More than 100 conidia were counted for each treatment.…”
Section: Determination Of the Rate Of Conidial Production And Germina...mentioning
BACKGROUND: Tea, one of the most important commercial crops on earth, is strongly affected by weeds on productivity and quality. Bioherbicides are shedding new light on weed control in tea gardens in an economical and safe manner.RESULTS: A pathogenic strain SYNJC-2-2 was isolated from diseased leaves of a noxious weed, goosegrass (Eleusine indica), from a tea garden in Zhejiang Province, China. It was identified as the fungal species Bipolaris bicolor based on the morphological characteristics and phylogenetic analysis. The potential of the B. bicolor strain SYNJC-2-2 as a bioherbicide was assessed by determining its efficacy to control weeds and selectivity to crops, its infection process and the influence of environmental conditions on conidial production and germination. The ED 90 (effective dose of conidia resulting in 90 disease index) of SYNJC-2-2 on goosegrass was 2 × 10 4 conidia mL −1 . Additionally, three Poaceae weeds, Setaria viridis, Microstegium vimineum and Pennisetum alopecuroides, were also extremely susceptible to SYNJC-2-2. SYNJC-2-2 was safe to 14 out of 17 crop species in nine families, especially tea plants. Conidial germination, hyphal growth and appressorial formation occurred within 3 to 6 h on goosegrass leaves. Hyphae invaded leaf tissues mainly through epidermal cell junctions and cracks, causing cell death and necrotic lesions within 2 days on inoculated leaves and killing goosegrass plants within 7 days. Furthermore, SYNJC-2-2 has a strong adaptability to environmental variables and high conidial production capacity on goosegrass juice agar media.CONCLUSION: Bipolaris bicolor strain SYNJC-2-2 has the potential to be developed as a bioherbicide for controlling goosegrass, especially in tea gardens.
“…This species occurs generally on plants in warm temperate and tropical regions 26,27 . There are reports of B. bicolor being isolated from plant leaf spot and root rot affected St. Augustine grass ( Stenotaphrum secundatum ), 28 signalgrass ( Brachiaria decumbens ), 29 hemp palm ( Trachycarpus fortunei ) 30 and the rubber tree ( Ficus elastica ) 31 . In 2017, although the strain CPC28811 of B. bicolor was first reported from new host goosegrass in Thailand, no descriptions about its morphological characteristics and disease symptoms were given 27 .…”
Section: Resultsmentioning
confidence: 99%
“…The reaction system with a 25 μL volume contained 12.5 μL 2 × Hieff PCR Master Mix with Dye (Yeasen Biotech Co., Ltd, Shanghai, China), 1 μL of each 10 μM primer, 1 μL of template DNA and 9.5 μL of sterilized distilled water. The PCR reaction programs were as follows: an initial denaturing step at 94 °C for 5 min, followed by 30…”
Section: Molecular Identification Of the Synjc-2-2 Strainmentioning
confidence: 99%
“…After 20-μL conidial suspensions of 5 × 10 5 were dripped onto a glass slide with a grooved surface and dried in air on a superclean bench, 20 μL of PDB liquid media (potato 200 g L −1 , sucrose 20 g L −1 ) with different pH (1-12) were added onto the grooved glass slides and incubated for 6 h at 28 °C and 100% relative humidity in darkness, or the dried conidia on grooved glass slides were directly placed in different relative humidity conditions (69%, 75%, 85%, 90%, 94%, 98% and 100%) and incubated for 12 h at 28 °C in darkness. To evaluate the effect of temperature and light on conidial germination, 20 μL of conidial suspensions of 5 × 10 5 were dripped onto the grooved glass slides and incubated for 6 h at various temperatures (15,20,25,30,35,40,45,49, 50 and 51 °C) under dark or at 28 °C under various illuminations (dark, fluorescence light, UV light and black light). More than 100 conidia were counted for each treatment.…”
Section: Determination Of the Rate Of Conidial Production And Germina...mentioning
BACKGROUND: Tea, one of the most important commercial crops on earth, is strongly affected by weeds on productivity and quality. Bioherbicides are shedding new light on weed control in tea gardens in an economical and safe manner.RESULTS: A pathogenic strain SYNJC-2-2 was isolated from diseased leaves of a noxious weed, goosegrass (Eleusine indica), from a tea garden in Zhejiang Province, China. It was identified as the fungal species Bipolaris bicolor based on the morphological characteristics and phylogenetic analysis. The potential of the B. bicolor strain SYNJC-2-2 as a bioherbicide was assessed by determining its efficacy to control weeds and selectivity to crops, its infection process and the influence of environmental conditions on conidial production and germination. The ED 90 (effective dose of conidia resulting in 90 disease index) of SYNJC-2-2 on goosegrass was 2 × 10 4 conidia mL −1 . Additionally, three Poaceae weeds, Setaria viridis, Microstegium vimineum and Pennisetum alopecuroides, were also extremely susceptible to SYNJC-2-2. SYNJC-2-2 was safe to 14 out of 17 crop species in nine families, especially tea plants. Conidial germination, hyphal growth and appressorial formation occurred within 3 to 6 h on goosegrass leaves. Hyphae invaded leaf tissues mainly through epidermal cell junctions and cracks, causing cell death and necrotic lesions within 2 days on inoculated leaves and killing goosegrass plants within 7 days. Furthermore, SYNJC-2-2 has a strong adaptability to environmental variables and high conidial production capacity on goosegrass juice agar media.CONCLUSION: Bipolaris bicolor strain SYNJC-2-2 has the potential to be developed as a bioherbicide for controlling goosegrass, especially in tea gardens.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.