Conspicuous endolithic algal associations in a mesophotic reef-building coral

González-Zapata, Fanny L.; Gómez-Osorio, Sebastián; Sánchez, Juan A.

doi:10.1007/s00338-018-1695-9

Cited by 22 publications

(20 citation statements)

References 26 publications

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“…Despite rapidly growing evidence of the high genetic diversity of the microboring Chlorophyta Ostreobium in living corals in reef ecosystems (Marcelino and Verbruggen, 2016; Sauvage et al ., 2016; Marcelino et al ., 2017; del Campo et al ., 2017; Gonzalez‐Zapata et al ., 2018), phenotypic characterization of specific Ostreobium genotypes has lagged behind. Indeed, phenotype studies have so far focused only on strains 6.99 and B14.86 designated as Ostreobium quekettii (Kornmann and Sahling, 1980; Bornet and Flahaut, 1889; Schlichter et al ., 1997; Krause et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Pioneer morphological observations showed that bioeroding filaments of the chlorophyte Ostreobium are ubiquitous inside the skeleton of tropical coral reef‐builders, both in dead and actively growing colonies (Odum and Odum, 1955; Lukas, 1974; Le Campion‐Alsumard et al ., 1995a; reviewed in Tribollet, 2008; Golubic et al ., 2019). Molecular data have recently accumulated, based on amplicon sequencing of plastid encoded gene markers ( rbc L, tuf A, UPA and 16S rRNA), revealing Ostreobium ubiquity in the core microbiome of tropical corals and its high genetic diversity, delimiting an entire Ostreobineae suborder within the Bryopsidales in the class Ulvophyceae (Gutner‐Hoch and Fine, 2011; Marcelino and Verbruggen, 2016; Sauvage et al ., 2016; del Campo et al ., 2017; Marcelino et al ., 2017; Verbruggen et al ., 2017; Gonzalez‐Zapata et al ., 2018; Marcelino et al ., 2018; Massé et al ., 2018). By chemical means, Ostreobium filaments actively penetrate reef carbonates ranging from limestone rocks to seashells and coral skeletons, creating galleries a few micrometres in diameter (Tribollet, 2008), thus living as true boring endoliths (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Functional diversity of microboringOstreobiumalgae isolated from corals

Massé

Tribollet

Méziane

et al. 2020

Environmental Microbiology

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Summary The filamentous chlorophyte Ostreobium sp. dominates shallow marine carbonate microboring communities, and is one of the major agents of reef bioerosion. While its large genetic diversity has emerged, its physiology remains little known, with unexplored relationship between genotypes and phenotypes (endolithic versus free‐living growth forms). Here, we isolated nine strains affiliated to two lineages of Ostreobium (>8% sequence divergence of the plastid gene rbcL), one of which was assigned to the family Odoaceae, from the fast‐growing coral host Pocillopora acuta Lamarck 1816. Free‐living isolates maintained their bioerosive potential, colonizing pre‐bleached coral carbonate skeletons. We compared phenotypes, highlighting shifts in pigment and fatty acid compositions, carbon to nitrogen ratios and stable isotope compositions (δ13C and δ15N). Our data show a pattern of higher chlorophyll b and lower arachidonic acid (20:4ω6) content in endolithic versus free‐living Ostreobium. Photosynthetic carbon fixation and nitrate uptake, quantified via 8 h pulse‐labeling with 13C‐bicarbonate and 15N‐nitrate, showed lower isotopic enrichment in endolithic compared to free‐living filaments. Our results highlight the functional plasticity of Ostreobium phenotypes. The isotope tracer approach opens the way to further study the biogeochemical cycling and trophic ecology of these cryptic algae at coral holobiont and reef scales.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Functional diversity of microboringOstreobiumalgae isolated from corals

Massé

Tribollet

Méziane

et al. 2020

Environmental Microbiology

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show abstract

“…Eukaryotic green algae are abundant in the skeletons of live corals, exceeding the biomass of Symbiodiniaceae in the tissue by 16 times [29]. The green alga Ostreobium is the most common genus, present in the vast majority of stony coral samples [2, 29–31]. Its simple morphology and the laborious nature of isolating and culturing endolithic green algae has limited our knowledge about the biodiversity of these organisms, but culture-independent sequencing approaches have recently shown a massive biodiversity of green algal endoliths in coral skeletons, including a lineage of about 80 different Ostreobium species and several other entirely unknown family-level lineages [13, 24], suggesting that distantly related green algal lineages may have gone under the name “ Ostreobium ” in previous studies.…”

Section: Diversity and Distribution Of The Skeletal Microbiomementioning

confidence: 99%

Beneath the surface: community assembly and functions of the coral skeleton microbiome

et al. 2019

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Coral microbial ecology is a burgeoning field, driven by the urgency of understanding coral health and slowing reef loss due to climate change. Coral resilience depends on its microbiota, and both the tissue and the underlying skeleton are home to a rich biodiversity of eukaryotic, bacterial and archaeal species that form an integral part of the coral holobiont. New techniques now enable detailed studies of the endolithic habitat, and our knowledge of the skeletal microbial community and its eco-physiology is increasing rapidly, with multiple lines of evidence for the importance of the skeletal microbiota in coral health and functioning. Here, we review the roles these organisms play in the holobiont, including nutritional exchanges with the coral host and decalcification of the host skeleton. Microbial metabolism causes steep physico-chemical gradients in the skeleton, creating micro-niches that, along with dispersal limitation and priority effects, define the fine-scale microbial community assembly. Coral bleaching causes drastic changes in the skeletal microbiome, which can mitigate bleaching effects and promote coral survival during stress periods, but may also have detrimental effects. Finally, we discuss the idea that the skeleton may function as a microbial reservoir that can promote recolonization of the tissue microbiome following dysbiosis and help the coral holobiont return to homeostasis.

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“…Despite rapidly growing evidence of the high genetic diversity of the microboring Chlorophyta Ostreobium in living corals in reef ecosystems Sauvage et al, 2016;Marcelino et al, 2017;del Campo et al, 2017;Gonzalez-Zapata et al, 2018), phenotypic characterization of specific Ostreobium genotypes has lagged behind.…”

Section: Diversity Of Ostreobium Strains From Pocillopora Coralsmentioning

confidence: 99%

“…Pioneer morphological observations showed that bioeroding filaments of the chlorophyte Ostreobium are ubiquitous inside the skeleton of tropical coral reef-builders, both in dead and actively growing colonies (Odum & Odum, 1955;Lukas, 1974;Le Campion-Alsumard et al, 1995a;reviewed in Tribollet, 2008;Golubic et al, 2019). Molecular data have recently accumulated, based on amplicon sequencing of plastid encoded gene markers (rbcL, tufA, UPA and 16S rRNA), revealing Ostreobium ubiquity in the core microbiome of tropical corals and its high genetic diversity, delimiting an entire Ostreobineae suborder within the Bryopsidales in the class Ulvophyceae (Gutner-Hoch & Fine, 2011;Sauvage et al, 2016;del Campo et al, 2017;Marcelino et al, 2017;Verbruggen et al, 2017;Gonzalez-Zapata et al, 2018;Marcelino et al, 2018;Massé et al, 2018). By chemical means, Ostreobium filaments actively penetrate reef carbonates ranging from limestone rocks to seashells and coral skeletons, creating galleries a few micrometers in diameter (Tribollet, 2008), thus living as true boring endoliths (i.e.…”

Section: Introductionmentioning

confidence: 99%

Functional diversity of microboringOstreobiumalgae isolated from corals

Massé

Tribollet

Méziane

et al. 2020

Preprint

View full text Add to dashboard Cite

The filamentous chlorophyte Ostreobium sp. dominates shallow marine carbonate microboring communities, and is one of the major agents of reef bioerosion. While its large genetic diversity has emerged, its physiology remains little known, with unexplored relationship between genotypes and phenotypes (endolithic versus free-living growth forms). Here, we isolated 9 strains affiliated to 2 lineages of Ostreobium (>8% sequence divergence of the plastid gene rbcL), one of which was assigned to the family Odoaceae, from the fast-growing coral host Pocillopora acuta Lamarck 1816. Free-living isolates maintained their bioerosive potential, colonizing pre-bleached coral carbonate skeletons. We compared phenotypes, highlighting shifts in pigment and fatty acid compositions, carbon to nitrogen ratios and stable isotope compositions (δ13C and δ15N). Our data show a pattern of higher chlorophyll b and lower arachidonic acid (20:4ω6) content in endolithic versus free-living Ostreobium. Photosynthetic carbon fixation and nitrate uptake, quantified via 8h pulse-labeling with 13C-bicarbonate and 15N-nitrate, showed lower isotopic enrichment in endolithic compared to free-living filaments. Our results highlight the functional plasticity of Ostreobium phenotypes. The isotope tracer approach opens the way to further study the biogeochemical cycling and trophic ecology of these cryptic algae at coral holobiont and reef scales.

show abstract

Conspicuous endolithic algal associations in a mesophotic reef-building coral

Cited by 22 publications

References 26 publications

Functional diversity of microboringOstreobiumalgae isolated from corals

Functional diversity of microboringOstreobiumalgae isolated from corals

Beneath the surface: community assembly and functions of the coral skeleton microbiome

Functional diversity of microboringOstreobiumalgae isolated from corals

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