Consolidated conversion of protein waste into biofuels and ammonia using Bacillus subtilis

Choi, Kwon‐Young; Wernick, David G.; Tat, Christine A.; Liao, James C.

doi:10.1016/j.ymben.2014.02.007

Cited by 89 publications

(46 citation statements)

References 51 publications

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“…4). This ammonia yield is 23% higher than that in the previous study (Choi et al 2014). Our study demonstrated that ammonia could be recovered in a low energy-consuming manner and that it would be possible to cover some of the increasing demand for ammonia in the future.…”

Section: Discussioncontrasting

confidence: 66%

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Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

et al. 2017

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The demand for ammonia is expected to increase in the future because of its importance in agriculture, industry, and hydrogen transportation. Although the Haber–Bosch process is known as an effective way to produce ammonia, the process is energy-intensive. Thus, an environmentally friendly ammonia production process is desired. In this study, we aimed to produce ammonia from amino acids and amino acid-based biomass-like resources by modifying the metabolism of Escherichia coli. By engineering metabolic flux to promote ammonia production using the overexpression of the ketoisovalerate decarboxylase gene (kivd), derived from Lactococcus lactis, ammonia production from amino acids was 351 mg/L (36.6% yield). Furthermore, we deleted the glnA gene, responsible for ammonia assimilation. Using yeast extract as the sole source of carbon and nitrogen, the resultant strain produced 458 mg/L of ammonia (47.8% yield) from an amino acid-based biomass-like material. The ammonia production yields obtained are the highest reported to date. This study suggests that it will be possible to produce ammonia from waste biomass in an environmentally friendly process.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0385-2) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 66%

“…Because E. coli cannot directly utilize proteins and long-chain peptides as a nutrient source, they must be decomposed into short-chain peptides before ammonia production (Huo et al 2011). This is done by heterologous expression of strong proteases in E. coli (Choi et al 2014; Su et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

et al. 2017

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“…These studies have often focused on the formation of compounds that contribute to the flavor profile of foods and beverages (branched-chain and aromatic aldehydes, alcohols, and acids) (Smit et al 2005(Smit et al , 2009. Recently, some studies have focused on the production of branched-chain alcohols from protein-rich waste using genetically engineered Escherichia coli and Bacillus subtilis with the main focus being that BCOHs are promising biofuel candidates (Huo et al 2011;Choi et al 2014). Additionally, BCOHs can be used as building blocks for the production of higher chemicals (Sakuragi et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Branched-chain alcohol formation by thermophilic bacteria within the genera of Thermoanaerobacter and Caldanaerobacter

et al. 2015

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Fifty-six thermophilic strains including members of Caldanaerobacter, Caldicellulosiruptor, Caloramator, Clostridium, Thermoanaerobacter, and Thermoanaerobacterium, were investigated for branched-chain amino acid degradation in the presence of thiosulfate in batch culture. All of the Thermoanaerobacter and Caldanaerobacter strains (24) degraded the branched-chain amino acids (leucine, isoleucine, and valine) to a mixture of their corresponding branched-chain fatty acids and branched-chain alcohols. Only one Caloramator strain degraded the branched-chain amino acids to the corresponding branched-chain fatty acids. The ratio of branched-chain fatty acid production over branched-chain alcohol production for Thermoanaerobacter was 7.15, 6.61, and 11.53 for leucine, isoleucine, and valine, respectively. These values for Caldanaerobacter were 3.49, 4.13, and 7.31, respectively. This indicates that members within Caldanaerobacter produce proportionally more of the alcohols as compared with Thermoanaerobacter. No species within other genera investigated produced branched-chain alcohols from branched-chain amino acids in the presence of thiosulfate.

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“…Ammonia (Figure 4e) is another bulk chemical used in many industries. Choi et al (65) engineered B. subtilis to produce biofuels (in the form of 2-keto acids) and ammonia from amino acid media at 18.9% and 46.6% of maximum theoretical yield with the overexpression of genes derived from other microbial sources under a strong inducible promoter.…”

Section: Commodity Chemicalsmentioning

confidence: 99%

Synthetic Biology for Specialty Chemicals

Markham¹,

Alper

2015

Annu. Rev. Chem. Biomol. Eng.

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In this review, we address recent advances in the field of synthetic biology and describe how those tools have been applied to produce a wide variety of chemicals in microorganisms. Here we classify the expansion of the synthetic biology toolbox into three different categories based on their primary function in strain engineering-for design, for construction, and for optimization. Next, focusing on recent years, we look at how chemicals have been produced using these new synthetic biology tools. Advances in producing fuels are briefly described, followed by a more thorough treatment of commodity chemicals, specialty chemicals, pharmaceuticals, and nutraceuticals. Throughout this review, an emphasis is placed on how synthetic biology tools are applied to strain engineering. Finally, we discuss organism and host strain diversity and provide a future outlook in the field.

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Consolidated conversion of protein waste into biofuels and ammonia using Bacillus subtilis

Cited by 89 publications

References 51 publications

Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Ammonia production from amino acid-based biomass-like sources by engineered Escherichia coli

Branched-chain alcohol formation by thermophilic bacteria within the genera of Thermoanaerobacter and Caldanaerobacter

Synthetic Biology for Specialty Chemicals

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