Consolidated bioprocessing for production of polyhydroxyalkanotes from red algae Gelidium amansii

Sawant, Shailesh S.; Salunke, Bipinchandra K.; Kim, Beom Soo

doi:10.1016/j.ijbiomac.2017.11.084

Cited by 24 publications

(12 citation statements)

References 54 publications

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“…If a process could be established that includes hydrolysis and fermentation in a single step, this would be a promising approach for producing PHA from marine biomass. Recently, Sawant et al succeeded in producing Saccharophagus degradans 2-40 P(3HB) from red algae without pretreatment (Sawant et al, 2018). In the cultivation of S. degradans 2-40 using the medium including untreated red algae, the P(3HB) content was from 12 to 16 wt%, which was a similar value to the P(3HB) content of our isolated strain using the MM medium including Laminaria sp.…”

Section: Polymersupporting

confidence: 77%

“…would be easily utilized for growth and P(3HB) production by the 5-11-6-3 strain in our future work. Some researches have focused on waste or inedible seaweeds such as red algae Gelidium amansii (Alkotaini et al, 2016;Sawant et al, 2018), green macroalgae Ulva sp. (Ghosh et al, 2019), and brown algae Sargassum sp.…”

Section: Polymermentioning

confidence: 99%

“…With respect to PHA production from seaweeds, studies have shown that PHA was accumulated by bacteria in a medium containing brown algae or compounds extracted from seaweed (levulinic acid) (Bera et al, 2015;Azizi et al, 2017). The red algae species Gelidium amansii and green macroalgae species Ulva have also been used for PHA production (Alkotaini et al, 2016;Sawant et al, 2018;Ghosh et al, 2019). In two of these reports (Alkotaini et al, 2016;Ghosh et al, 2019), seaweeds that were chemically hydrolyzed and/or enzymatically saccharified were used for PHA production.…”

Section: Introductionmentioning

confidence: 99%

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Cobetia sp. Bacteria, Which Are Capable of Utilizing Alginate or Waste Laminaria sp. for Poly(3-Hydroxybutyrate) Synthesis, Isolated From a Marine Environment

Moriya

Takita

Matsumoto

et al. 2020

Front. Bioeng. Biotechnol.

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We isolated the Cobetia sp. strains IU 180733JP01 (5-11-6-3) and 190790JP01 (5-25-4-2) from seaweeds and showed that both strains accumulate poly(3-hydroxybutyrate) [P(3HB)] homopolymer in a nitrogen-limiting mineral salt medium containing alginate as a sole carbon source. Genome sequence analysis of the isolated strains showed that they have putative genes which encode enzymes relevant to alginate assimilation and P(3HB) synthesis, and the putative alginate-assimilating genes formed a cluster. Investigation of the optimum culture conditions for high accumulation of P(3HB) showed that when the 5-11-6-3 strain was cultured in a nitrogen-limiting mineral salt medium (pH 5.0) containing 6% NaCl and 3% (w/v) alginate as a sole carbon source for 2 days, the P(3HB) content and P(3HB) production reached 62.1 ± 3.4 wt% and 3.11 ± 0.16 g/L, respectively. When the 5-25-4-2 strain was cultured in a nitrogenlimiting mineral salt medium (pH 4.0) containing 5% NaCl and 3% (w/v) alginate for 2 days, the P(3HB) content and P(3HB) production reached 56.9 ± 2.1 wt% and 2.67 ± 0.11 g/L, respectively. Moreover, the 5-11-6-3 strain also produced P(3HB) in a nitrogen-limiting mineral salt medium (pH 5.0) containing 6% NaCl and freeze-dried and crushed waste Laminaria sp., which is classified into brown algae and contains alginate abundantly. The resulting P(3HB) content and P(3HB) productivity were 13.5 ± 0.13 wt% and 3.99 ± 0.15 mg/L/h, respectively. Thus, we demonstrated the potential application of the isolated strains to a simple P(3HB) production process from seaweeds without chemical hydrolysis and enzymatic saccharification.

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Section: Polymersupporting

confidence: 77%

Section: Polymermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cobetia sp. Bacteria, Which Are Capable of Utilizing Alginate or Waste Laminaria sp. for Poly(3-Hydroxybutyrate) Synthesis, Isolated From a Marine Environment

Moriya

Takita

Matsumoto

et al. 2020

Front. Bioeng. Biotechnol.

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show abstract

“…However, recently seaweed has been explored as a potential substrate for PHA production. Studies have shown that bacteria accumulated PHA in a medium containing brown algae (Azizi et al, 2017;Moriya et al, 2020;Muhammad et al, 2020), red algae (Alkotaini et al, 2016;Bera et al, 2015;Sawant et al, 2018), and green seaweed Ulva sp. (Ghosh et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Gnaim

Polikovsky

Unis

et al. 2021

Bioresource Technology

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The biosynthesis of polyhydroxyalkanoate (PHA) biopolymers from certain marine microbes, associated with green macroalgae Ulva sp., has attracted significant attention. The Ulva sp. is abundant biomass in numerous locations around the world and could be easily cultivated by marine farming. The variety of sugars found in Ulva sp. homogenate could be used as a carbon source for microbial growth and PHA production. In this work, we isolated and explored a series of bacterial strains that function as potential producers of P(3HB), utilizing a range of common sugars found in Ulva sp. Analysis of 16S rDNA gene-sequence revealed that the PHA-producing bacteria were phylogenetically related to species of the genus Cobetia, Bacillus, Pseudoaltermonas, and Sulfitobacter. The highest-yield of P(3HB) was observed in the case of new Cobetia strain, C. amphilecti, with up to 61% (w/w) in the presence of mannitol and 12% (w/w) on Ulva sp. acid hydrolysate as a substrate.

show abstract

Section: Introductionmentioning

confidence: 99%

Marine Bacteria Associated with the Green SeaweedUlvasp. for the Production of Polyhydroxyalkanoates

Gnaim

Polikovsky

Unis

et al. 2020

Preprint

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The biosynthesis of polyhydroxyalkanoate (PHA) biopolymers from certain marine microbes, associated with green macroalgae Ulva sp., has attracted significant attention. The Ulva sp. is abundant biomass in numerous locations around the world and could be easily cultivated by marine farming. The variety of sugars found in Ulva sp. homogenate could be used as a carbon source for microbial growth and PHA production. In this work, we isolated and explored a series of bacterial strains that function as potential producers of P(3HB), utilizing a range of common sugars found in Ulva sp. Analysis of 16S rDNA gene-sequence revealed that the PHA-producing bacteria were phylogenetically related to species of the genus Cobetia, Bacillus, Pseudoaltermonas, and Sulfito-bacter. The highest-yield of P(3HB) was observed in the case of new Cobetia strain, C. amphilecti, with up to 61% (w/w) in the presence of mannitol and 12% (w/w) on Ulva sp. acid hydrolysate as a substrate.

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Consolidated bioprocessing for production of polyhydroxyalkanotes from red algae Gelidium amansii

Cited by 24 publications

References 54 publications

Cobetia sp. Bacteria, Which Are Capable of Utilizing Alginate or Waste Laminaria sp. for Poly(3-Hydroxybutyrate) Synthesis, Isolated From a Marine Environment

Cobetia sp. Bacteria, Which Are Capable of Utilizing Alginate or Waste Laminaria sp. for Poly(3-Hydroxybutyrate) Synthesis, Isolated From a Marine Environment

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Marine Bacteria Associated with the Green SeaweedUlvasp. for the Production of Polyhydroxyalkanoates

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