Consistent association of crenarchaeal Archaea with sponges of the genus Axinella

Margot, H.; Acebal, C.; Toril, Elena González; Amils, Ricardo; Puentes, José L. Fernández

doi:10.1007/s00227-001-0740-2

Cited by 65 publications

(4 citation statements)

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“…All of the archaeal sequences in Cinachyrella fell within two phyla: Thaumarchaeota and Euryarchaeota , with most of the archaea belonging to the Thaumarchaeota , which is widespread in sponges (Webster et al, 2001; Margot et al, 2002; Lee et al, 2011; Kennedy et al, 2014; Polónia et al, 2014). Archaeal reads grouped into a low number of OTUs, with a few numerically dominant ones, similar to the four species sampled by Kennedy et al (2014), which had 70% of the Thaumarchaeota sequences separated in three OTUs.…”

Section: Discussionmentioning

confidence: 99%

Two distinct microbial communities revealed in the sponge Cinachyrella

et al. 2014

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Marine sponges are vital components of benthic and coral reef ecosystems, providing shelter and nutrition for many organisms. In addition, sponges act as an essential carbon and nutrient link between the pelagic and benthic environment by filtering large quantities of seawater. Many sponge species harbor a diverse microbial community (including Archaea, Bacteria and Eukaryotes), which can constitute up to 50% of the sponge biomass. Sponges of the genus Cinachyrella are common in Caribbean and Floridian reefs and their archaeal and bacterial microbiomes were explored here using 16S rRNA gene tag pyrosequencing. Cinachyrella specimens and seawater samples were collected from the same South Florida reef at two different times of year. In total, 639 OTUs (12 archaeal and 627 bacterial) belonging to 2 archaeal and 21 bacterial phyla were detected in the sponges. Based on their microbiomes, the six sponge samples formed two distinct groups, namely sponge group 1 (SG1) with lower diversity (Shannon-Weiner index: 3.73 ± 0.22) and SG2 with higher diversity (Shannon-Weiner index: 5.95 ± 0.25). Hosts' 28S rRNA gene sequences further confirmed that the sponge specimens were composed of two taxa closely related to Cinachyrella kuekenthalli. Both sponge groups were dominated by Proteobacteria, but Alphaproteobacteria were significantly more abundant in SG1. SG2 harbored many bacterial phyla (>1% of sequences) present in low abundance or below detection limits (<0.07%) in SG1 including: Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospirae, PAUC34f, Poribacteria, and Verrucomicrobia. Furthermore, SG1 and SG2 only had 95 OTUs in common, representing 30.5 and 22.4% of SG1 and SG2's total OTUs, respectively. These results suggest that the sponge host may exert a pivotal influence on the nature and structure of the microbial community and may only be marginally affected by external environment parameters.

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Section: Discussionmentioning

confidence: 99%

Two distinct microbial communities revealed in the sponge Cinachyrella

et al. 2014

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“…The closest sequence retrieved by blast search of our ZOTU2 (Cenarchaeum) had 96.5% identity to the C. symbiosum clone (Ar20-3) found in an unidentified marine sponge from Korea (AY192631.1). Thus, although different phylotypes of this archaeon might exist (Margot et al, 2002), its presence in all sponge samples gives support to the cosmopolitan distribution of Cenarchaeum in sponges and points to a relevant role in the holobiont. However, the exclusive presence of C. symbiosum reported in some sponges, such as Petromica citrina (Turque et al, 2010) and A. mexicana (Preston et al, 1996), is rather an exceptional pattern in our samples.…”

Section: Sponge Archaeal Communitiesmentioning

confidence: 64%

“…Sponges, together with corals, are paradigmatic representatives of the holobiont concept (Dittami et al, 2019) in marine ecosystems. Sponges establish tight associations simultaneously with members of the three domains of life: Eukarya (Wulff, 2006;De Mares et al, 2017;Turon et al, 2019b), Bacteria (Taylor et al, 2007;Pita et al, 2018;Turon et al, 2018Turon et al, , 2019a and Archaea (Margot et al, 2002;Simister et al, 2012;Zhang et al, 2014;Rodríguez-Marconi et al, 2015;De Mares et al, 2017). Sponge-associated bacteria are particularly abundant in the socalled bacteriosponges (Vacelet and Donadey, 1977) or High Microbial Abundant sponges.…”

Section: Introductionmentioning

confidence: 99%

“…However, regardless the apparently lower abundance of archaea in sponge microbiomes with respect to bacteria, some archaea have been recognized to play chief roles in the holobiont functioning (Radax et al, 2012b;Tian et al, 2014;Moeller et al, 2019). Thaumarchaetoa is the most abundant phylum reported among the sponge-associated archaea (Margot et al, 2002;Hoffmann et al, 2009;Radax et al, 2012b;Moeller et al, 2019). Some members of this Phylum are key chemoautotrophic ammonia-oxidizers microorganisms in marine habitats (Pester et al, 2012) and have been suggested to play a role in ammonia detoxification within the host sponges (Steger et al, 2008;Hoffmann et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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New Insights Into the Archaeal Consortium of Tropical Sponges

Turon

Uriz

2020

Front. Mar. Sci.

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Archaea is a poorly studied domain associated with sponges. Many questions that have been addressed for bacteria still remain largely unknown for archaea. In this study, we analyzed the archaeal communities of 17 tropical sponge species from Nha Trang Bay (Vietnam) using archaea specific primers. We recorded patterns of diversity and spatial stability of these microbial communities and compared the results obtained with the bacterial communities, already reported in our previous study. In our study species, Shannon diversity was always lower for archaeal than for bacterial communities. The differences in alpha diversity as well as the presence of indicator phyla reported for the bacterial domain in High Microbial Abundance (HMA) and Low Microbial Abundance (LMA) could not be confirmed for the archaeal communities. Host identity was the main factor structuring the archaeal assemblages. Sponge archaea core was formed by few but very abundant ZOTUs, which contribute with a high proportion to the relative archaea abundance. The inclusion of the obtained sequences into phylogenetic trees allowed finding out whether or not they belonged to the socalled sponge clusters (SC). Our results showed that most of the Thaumarchaeota and Euryarchaeota sequences were more closely related to environmental samples than to SC, suggesting that they might be acquired from the seawater, which need to be verified. However, representatives of Woesarchaeota, which were major members of the archaea microbiome of two sponge species, formed a monophyletic tree, distantly related to any known environmental sequence.

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Archaea

Thiel¹

2011

Encyclopedia of Geobiology

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Consistent association of crenarchaeal Archaea with sponges of the genus Axinella

Cited by 65 publications

References 32 publications

Two distinct microbial communities revealed in the sponge Cinachyrella

Two distinct microbial communities revealed in the sponge Cinachyrella

New Insights Into the Archaeal Consortium of Tropical Sponges

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