Consistency of population genetics parameters estimated from isozyme and RAPDs dataset in species of genus Prosopis (Leguminosae, Mimosoideae)

Abstract

Genetic variability, population structure and differentiation among 17 populations of 5 species and 2 natural interspecific hybrids of section Algarobia of genus Prosopis were analyzed from data of 23 isozyme and 28 RAPD loci. Both markers indicated that the studied populations are highly variable. P. alba populations in average showed lower values of genetic variability estimates from isozyme data, but this trend was not observed for RAPD markers. The hierarchical analyses of the distribution of genetic variability showed that the highest proportion of variation occurred within populations, the differentiation among species was intermediate and the lowest component was observed among populations within species. The consistency between results from both dataset implies that they are not biased and reflect the actual genetic structure of the populations analyzed. The matrices of Euclidean distances obtained from the two sets of markers were highly correlated according to Mantel test. In both cases the corresponding phenogram and MDS plot tended to cluster conspecific populations while hybrid populations were not intermediate between putative parents. Some disagreements between isozyme and RAPD phenograms were observed mainly in the affinities of hybrid populations. Such inconsistencies might result from reticular rather than dichotomic evolutionary relationships. The phenetic associations retrieved gave no support to the division of the section Algarobia into series.

Single primer amplification reaction (SPAR) reveals intra-specific natural variation in Prosopis cineraria (L.) Druce

“…Ferreyra et al (2004Ferreyra et al ( , 2007 reported that RAPD patterns able to differentiate some Argentinean Prosopis species of section Algarobia. Similarly, variation in isozyme pattern (Saidman and Vilardi 1987), and quantum of seed lipids (Lamarque et al 1994), mineral, crude protein, and structural carbohydrate (Arya et al 1995) have also been studied for distinguishing species.…”

The Fruits of Tropical Plant Genomics

“…Analyses of nuclear ribosomal internal transcribed spacers among various series of Prosopis suggested that some species were monophyletic as a result of independent speciation that widely occurred in the New World [12]. RAPD and isozyme data for various populations of Prosopis showed the greatest source of variation to be within populations [57]. With the exception of the atypical shrubby P. strombulifera, with low gene heterozygosity and which spreads by rhizomes [80], all the species studied to date are self-incompatible [87,154].…”

RAPD and microsatellite transferability studies in selected species of Prosopis (section Algarobia) with emphasis on Prosopis juliflora and P. pallida

“…Although isozyme analysis proved to be useful, the enzymatic expression is restricted to specific developmental stages or tissues influenced by the environment (Solbrig and Bawa 1975;Whitmore and Bragg 1979;Saidman and Vilardi 1987). However, RAPD markers have shown clear advantages over isozymes in detecting genetic variability between species within the genus (Goswami and Ranade 1999;Bessega et al 2000;Ferreyra et al 2007).…”