Considerations for large-scale implementation of dormant budwood cryopreservation

Tanner, Justin D.; Chen, Katheryn Y.; Bonnart, Remi; Minas, Ioannis S.; Volk, Gayle M.

doi:10.1007/s11240-020-01884-5

Cited by 16 publications

(21 citation statements)

References 71 publications

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“…Moreover, a dormant bud protocol may be easier to implement compared to in vitro-based protocols, which may need considerable optimization before they can be applied in practice [39,53,54]. If the recovery of cryopreserved buds is possible by grafting or direct rooting, the whole cryopreservation process can be done without laboratory facilities [9,20]. However, if laboratory facilities are available, the recovery via in vitro offers an opportunity to revive cryopreserved material throughout the year and makes it possible to multiply plantlets via micropropagation [13].…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the bud type, i.e., floral or vegetative, had a strong effect on the success of recovery, with vegetative buds giving a better result than floral buds. For dormant bud cryopreservation, twigs from the previous season’s growth with vegetative buds are usually recommended [ 9 , 14 ]. Moreover, the cold hardiness and cold acclimation state of source plants is considered the most important issue affecting the success of dormant bud cryopreservation [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

“…For the long-term preservation of cold-tolerant woody plant species, cryopreservation of dormant buds may be an applicable method [ 8 , 9 ]. The success of dormant bud cryopreservation is affected by the quality of the source material, the steps of the protocol, and recovery practices [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…After that, the cryopreservation of dormant buds was studied with species such as apple ( Malus domestica ) [ 11 , 12 ] and mulberry ( Morus bombycis Koidz.) [ 13 ], and to date, many cryoprotocols utilizing dormant buds with different recovery practices have been developed [ 8 , 9 ]. In the case of Malus , the recovery of buds is often done by grafting [ 14 ], but the recovery of buds via in vitro can also be used as in the case of species such as Ulmus [ 15 ], Diospyros kaki Thunb.…”

Section: Introductionmentioning

confidence: 99%

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Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

Rantala

Kaseva

Nukari

et al. 2021

Plants

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The cryopreservation of dormant buds can be a feasible method for preserving germplasm of cold-tolerant woody plants. In the present study, we evaluated the effects of pre-desiccation, thawing method, and the rehydration of bud sections on the post-cryopreservation recovery of dormant blackcurrant buds in vitro. The estimated recovery of small- and medium-sized buds was 80.1 and 62.7% respectively for desiccated buds and 67.8 and 72.3% respectively for non-desiccated buds. The pre-desiccation of bud sections enhanced the number of the shoots regenerated from vegetative buds (2.3 vs. 4.7). The estimated recovery of fast-thawed buds was better after 14-day than after 7-day rehydration (85 vs. 59%). In slowly thawed buds the difference between 14-day and 7-day rehydration was not significant (73 vs. 62%). The estimated recovery of vegetative and flower buds was 77.7 and 41.1% respectively after 7-day rehydration, and 95.2 and 43.6% respectively after a 14-day rehydration period. The rehydration of bud sections was not necessary for the in vitro recovery of non-desiccated, fast-thawed buds. Of the 23 blackcurrant cultivars cryopreserved using non-desiccated dormant buds collected from a greenhouse, the estimated recovery of 22 cultivars ranged between 42 and 90%.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

Rantala

Kaseva

Nukari

et al. 2021

Plants

View full text Add to dashboard Cite

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“…Therefore, various tactics are necessary to reduce marker expression by using derivable promoters or generating plants without markers (Pandey et al, 2020;Penna, Sági & Swennen, 2002). The production of transgenic plants takes a long time, is labor intensive, expensive and obviously not an efficient process (Miki & McHugh, 2004;Tanner et al, 2020). This is the situation when dealing with important agricultural crops.…”

Section: Need For Marker Gene Selectable Systemsmentioning

confidence: 99%

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

Dormatey

Sun

Ali

et al. 2021

PeerJ

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Antibiotic and herbicide resistance genes are the most common marker genes for plant transformation to improve crop yield and food quality. However, there is public concern about the use of resistance marker genes in food crops due to the risk of potential gene flow from transgenic plants to compatible weedy relatives, leading to the possible development of “superweeds” and antibiotic resistance. Several selectable marker genes such as aph, nptII, aaC3, aadA, pat, bar, epsp and gat, which have been synthesized to generate transgenic plants by genetic transformation, have shown some limitations. These marker genes, which confer antibiotic or herbicide resistance and are introduced into crops along with economically valuable genes, have three main problems: selective agents have negative effects on plant cell proliferation and differentiation, uncertainty about the environmental effects of many selectable marker genes, and difficulty in performing recurrent transformations with the same selectable marker to pyramid desired genes. Recently, a simple, novel, and affordable method was presented for plant cells to convert non-metabolizable phosphite (Phi) to an important phosphate (Pi) for developing cells by gene expression encoding a phosphite oxidoreductase (PTXD) enzyme. The ptxD gene, in combination with a selection medium containing Phi as the sole phosphorus (P) source, can serve as an effective and efficient system for selecting transformed cells. The selection system adds nutrients to transgenic plants without potential risks to the environment. The ptxD/Phi system has been shown to be a promising transgenic selection system with several advantages in cost and safety compared to other antibiotic-based selection systems. In this review, we have summarized the development of selection markers for genetic transformation and the potential use of the ptxD/Phi scheme as an alternative selection marker system to minimize the future use of antibiotic and herbicide marker genes.

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Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation

et al. 2022

Self Cite

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Plant cryopreservation technologies are used within gene banks for the long‐term preservation of vegetatively propagated collections. Surface‐sterilized plant tissues grown in the field, greenhouse/screenhouse, growth chamber, or in vitro are the source of shoot tips subjected to vitrification‐based cryopreservation methods. Here, we describe the methods used to minimize microbial contamination during the tissue culture initiation process. We also discuss the occurrence and possible elimination of endophytes after extended in vitro culture and during recovery after liquid nitrogen exposure. We describe two case studies in which bacterial endophytes were observed in Citrus gene bank accessions during recovery after cryopreservation. These were identified using the MinION Oxford Nanopore system and Kirby–Bauer disc diffusion assays to examine the bacterial responses to antibiotic exposure. The methods used in this case study could be applied to identify endophytes to better target antimicrobial treatments of plant tissue collections.

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Considerations for large-scale implementation of dormant budwood cryopreservation

Cited by 16 publications

References 71 publications

Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

Successful Cryopreservation of Dormant Buds of Blackcurrant (Ribes nigrum L.) by Using Greenhouse-Grown Plants and In Vitro Recovery

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation

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