Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples

Pimentel, Lígia L.; Fontes, Ana Luiza; Gomes, Ana; Rodriguez‐Alcalá, Luís M.

doi:10.1016/j.mex.2015.11.006

Cited by 14 publications

(12 citation statements)

References 7 publications

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“…Total amino acid content of each powder was determined using pre‐column derivatization with orthophthalaldehyde (OPA) methodology 35 and quantified using a calibration curve built with amino acid pure standards. The profile of fatty acids was obtained and analysed following the methodology of Pimentel et al 36 . with some modifications regarding the internal standard used (glyceryl tritridecanoine, TG‐C13) and the derivatization process (methanol and sodium methoxide were added to 50 mg of sample in amounts of 2.26 mL and 240 μL, respectively).…”

Section: Methodsmentioning

confidence: 99%

Are olive pomace powders a safe source of bioactives and nutrients?

et al. 2020

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BACKGROUND The olive oil industry generates significant amounts of semi‐solid wastes, namely olive pomace. Olive pomace is a by‐product rich in high‐value compounds (e.g. dietary fibre, unsaturated fatty acids, polyphenols) widely explored to obtain new food ingredients. However, conventional extraction methods frequently use organic solvents, while novel eco‐friendly techniques have high operational costs. The development of powdered products without any extraction step has been proposed as a more feasible and sustainable approach. RESULTS The present study fractionated and valorized the liquid and pulp fraction of olive pomace obtaining two stable and safe powdered ingredients, namely a liquid‐enriched powder (LOPP) and a pulp‐enriched powder (POPP). These powders were characterized chemically, and their bioactivity was assessed. LOPP exhibited a significant amount of mannitol (141 g kg−1), potassium (54 g kg−1) and hydroxytyrosol derivatives (5 mg g−1). POPP exhibited a high amount of dietary fibre (620 g kg−1) associated with a significant amount of bound phenolics (7.41 mg GAE g−1 fibre DW) with substantial antioxidant activity. POPP also contained an unsaturated fatty acid composition similar to that of olive oil (76% of total fatty acids) and showed potential as a reasonable source of protein (12%). Their functional properties (solubility, water‐holding and oil‐holding capacity), antioxidant capacity and antimicrobial activity were also assessed, and their biological safety was verified. CONCLUSIONS The development of olive pomace powders for application in the food industry could be a suitable strategy to add value to olive pomace and obtain safe multifunctional ingredients with higher health‐promoting effects than dietary fibre and polyphenols. © 2020 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Are olive pomace powders a safe source of bioactives and nutrients?

et al. 2020

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“…Thereafter, cells were pelleted (7,000 × g, 7 10 min, °C), rinsed twice with PBS, and stored at -80 ºC. For the quantification of total fatty acids (FA), 100 mg of sample (pellet) were accurately weighed and analysed as described by Pimentel et al (2015).…”

Section: Fatty Acid Analysismentioning

confidence: 99%

Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains

et al. 2020

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“…For the fatty acids (FA) analysis, stock solutions, supernatants and fermented milk samples (500 μL) or pellets (500 mg) were prepared according to Pimentel et al . 57 . Briefly, for quantification purposes, samples were added with 100 µL of tritridecanoin (1.34 mg/mL) and undecanoic acid (1.5 mg/mL) prior to derivatization.…”

Section: Methodsmentioning

confidence: 99%

Effect of Pufa Substrates on Fatty Acid Profile of Bifidobacterium breve Ncimb 702258 and CLA/CLNA Production in Commercial Semi-Skimmed Milk

Fontes

Pimentel

Rodriguez‐Alcalá

et al. 2018

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Current research on lipids is highlighting their relevant role in metabolic/signaling pathways. Conjugated fatty acids (CFA), namely isomers of linoleic and linolenic acid (i.e. CLA and CLNA, respectively) can positively modulate inflammation processes and energy metabolism, promoting anti-carcinogenic and antioxidant effects, improved lipid profiles and insulin resistance, among others. Bioactive doses have been indicated to be above 1 g/d, yet these cannot be achieved through a moderate intake (i.e. 1–2 servings) of natural sources, and certain CLA-containing products have limited commercial availability. Such handicaps have fueled research interest in finding alternative fortification strategies. In recent years, screening of dairy products for CFA-producing bacteria has attracted much attention and has led to the identification of some promising strains, including Bifidobacterium breve NCIMB 702258. This strain has shown interesting producing capabilities in model systems as well as positive modulation of lipid metabolism activities in animal studies. Accordingly, the aim of this research work was to assay B. breve NCIMB 702258 in semi-skimmed milk to produce a probiotic fermented dairy product enriched in bioactive CLA and CLNA. The effect of substrates (LA, α-LNA and γ-LNA) on growth performance and membrane fatty acids profile was also studied, as these potential modifications have been associated to stress response. When tested in cys-MRS culture medium, LA, α-LNA and γ-LNA impaired the fatty acid synthesis by B. breve since membrane concentrations for stearic and oleic acids decreased. Variations in the C18:1 c11 and lactobacillic acid concentrations, may suggest that these substrates are also affecting the membrane fluidity. Bifidobacterium breve CFA production capacity was first assessed in cys-MRS with LA, α-LNA, γ-LNA or all substrates together at 0.5 mg/mL each. This strain did not produce CFA from γ-LNA, but converted 31.12% of LA and 68.20% of α-LNA into CLA and CLNA, respectively, after incubation for 24 h at 37 °C. In a second phase, B. breve was inoculated in a commercial semi-skimmed milk with LA, α-LNA or both at 0.5 mg/mL each. Bifidobacterium breve revealed a limited capacity to synthesize CLA isomers, but was able to produce 0.062–0.115 mg/mL CLNA after 24 h at 37 °C. However, organoleptic problems were reported which need to be addressed in future studies. These results show that although CFA were produced at too low concentrations to be able to achieve solely the bioactive dose in one daily portion size, fermented dairy products are a suitable vector to deliver B. breve NCIMB 702258.

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Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples

Abstract: Graphical abstractIn situ derivatization to methyl esters (FAME) and fractionation of EFA and NEFA.

Cited by 14 publications

References 7 publications

Are olive pomace powders a safe source of bioactives and nutrients?

Are olive pomace powders a safe source of bioactives and nutrients?

Impact of exposure to cold and cold-osmotic stresses on virulence-associated characteristics of Listeria monocytogenes strains

Effect of Pufa Substrates on Fatty Acid Profile of Bifidobacterium breve Ncimb 702258 and CLA/CLNA Production in Commercial Semi-Skimmed Milk

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