Consideration of pulse-width effects of nanosecond pulsed electric fields application on cancer cell

Yamamoto, Yoshihiko; Ogura, Shun; Katsura, H.; Teranishi, Kenji; Uto, Yoshihiro; Shimomura, Naoyuki

doi:10.1109/ppc.2017.8291240

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…[29][30][31][32][33][34][35] Besides, our group measured cell surviving fraction and FACS in vitro in mouse-derived mammary carcinoma EMT-6 cells exposed to pulsed electric field with pulse width of 2 ns, 35 and conducted experiments with changing the cell type to melanoma cells B16-F10. 36 The present study explored synergistic effect of anticancer drug (Adriamycin) and application of nsPEFs. As a preliminary to animal experiments (in vivo) using embryonated chick essay, we conducted experiments (in vitro) on cell suspension in order to acquire basic information.…”

Section: Introductionmentioning

confidence: 99%

“…In our laboratory, nsPEFs have been applied to solid tumors using embryonated chick assay 29–35 . Besides, our group measured cell surviving fraction and FACS in vitro in mouse‐derived mammary carcinoma EMT‐6 cells exposed to pulsed electric field with pulse width of 2 ns, 35 and conducted experiments with changing the cell type to melanoma cells B16‐F10 36 . The present study explored synergistic effect of anticancer drug (Adriamycin) and application of nsPEFs.…”

Section: Introductionmentioning

confidence: 99%

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Effects of nanosecond pulsed electric fields application on cancer cell and combination of anticancer drug

Enomoto

Konishi

Uto

et al. 2022

Electrical Engineering Japan

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With the goal of establishing a new cancer treatment method using nanosecond pulsed electric fields (nsPEFs), we investigated the effect of applying nsPEFs and anticancer drugs (adriamycin, ADM) to cancer cells. The surviving fraction of cancer cells was significantly reduced by nsPEFs application compared to the control. Compared to nsPEFs alone and ADM administration alone, a significant decrease of the surviving fraction was observed in combination of nsPEFs application and ADM administration. In addition, multiplying value of surviving fractions of nsPEFs application and ADM administration was calculated as a pseudo-combination effect. The surviving fractions of the experimental combinational results of nsPEFs and ADM were lower than the pseudo-combination results. Therefore, the synergistic effect of combination of nsPEFs and ADM was confirmed. Furthermore, the surviving fraction of ADM administration after nsPEFs application was significantly lower than that of ADM administration on 24 h before nsPEFs application. ADM action on cells would be effective owing to nanopores were formed by applying nsPEFs. It was confirmed that ADM and nsPEF induced apoptosis of cells from apoptosis and necrosis test using FACS.

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