Conserving the gene pool of Far Eastern plants by means of seed cryopreservation

Kholina, A. B.; Воронкова, Н. М.

doi:10.1134/s1062359008030060

Cited by 11 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar response was reported for several orchid seeds (Nikishina et al, 2007;Popov et al, 2004); for Pyrus communis (Reed et al, 2001), Halimium atriplicifolium, Helianthemum apenninum, Helianthemum squamatum (P erez-García and Gonz alez-Benito, 2008), Angelica ursina, Oxytropis chankaenis, Oxytropis retusa (Kholina and Voronkova, 2008), Elaeis guineensis (Camillo et al, 2009), Passiflora suberosa, and Passiflora edulis (Ara ujo et al, 2016); and for wild medicinal legume species (Kholina and Voronkova, 2012) after seed cryostorage. It has been suggested that LN exposure may enhance the germination of treated seeds by breaking seed dormancy or softening of the seed coat (Kholina and Voronkova, 2008;Reed et al, 2001). In many species, exposition of seeds to low temperatures decreases the endogen content of abscisic acid and increases the gibberellin and cytokinin levels, which interact in a sequential way to break dormancy (Bewley and Black, 1994).…”

Section: Discussionsupporting

confidence: 69%

“…Cryopreservation prevents the depletion of reserve substances, accumulation of toxins, decomposition and inactivation of enzyme complexes, and autoxidation of lipids (Stanwood, 1985), thereby avoiding the risk of genetic and epigenetic changes (Bonner, 1990). Thus, seed cryobanks are promising for conserving the biological diversity of plant species and for maintaining the stability of their genotypes (Kholina and Voronkova, 2008).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Dolce¹,

Medina²,

González-Arnao

2018

horts

View full text Add to dashboard Cite

This research aimed at evaluating the desiccation tolerance and ability to withstand cryostorage of intact seeds of seven South American Ilex species, and comparing different methodologies for in vitro germination of fresh and cryostored seeds. Intact seeds were silica gel–desiccated from 2 to 14 hours, placed in cryovials, and immersed in liquid nitrogen (LN). Survival was assessed through in vitro germination of intact seeds, bisected seeds, or isolated embryos. Seeds of the seven Ilex species (Ilex brasiliensis (Sprengel) Loes., Ilex brevicuspis R., Ilex dumosa var. dumosa R., Ilex integerrima (Vell. Conc.) R., Ilex paraguariensis A. St. Hil., Ilex pseudoboxus R., and Ilex theezans R.) tolerated desiccation to ≈6% moisture content (MC) and could be successfully cryopreserved when MC decreased between 6.4% and 8.4% depending on the species, before immersion in LN. In addition, it was established as the optimal condition for in vitro seed germination of the seven Ilex species. A simple and cost-effective cryogenic procedure (which did not require the use of cryoprotectants or sophisticated facilities) was defined for seeds of seven Ilex species, which provides a new alternative for safe long-term preservation of Ilex germplasm.

show abstract

Section: Discussionsupporting

confidence: 69%

mentioning

confidence: 99%

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Dolce¹,

Medina²,

González-Arnao

2018

horts

View full text Add to dashboard Cite

show abstract

“…The seeds of the studied species have a hard seed coat with a layer of macrosclereids (palisade-like cells with strongly lignified walls), typical for many wild Fabaceae species [24,25], and most part of them is characterized by high and moderate levels of hardseededness (Table 2). A physical dormancy often occurs as a means of adaptation to the different habitats with the severe climatic conditions, and such seeds are highly resistant to unfavorable environmental factors [26,27]. It is low permeability of hard seed coat that may play some role in the cryotolerance of these seeds [19,28].…”

Section: Discussionmentioning

confidence: 99%

Seed Cryopreservation of Some Medicinal Legumes

Kholina

Воронкова

2012

Journal of Botany

View full text Add to dashboard Cite

Seed survival after storage in liquid nitrogen (–196°C) was examined in 12 wild medicinal legume species occurred Far East of Russia. Dry seeds of all species survived cryostorage without loss of viability. Initial germinability varied from 3 to 85%. The stimulatory effect of cryogenic temperature on germination, with or without subsequent chemical scarification, was observed in all species studied with deep physical dormancy or heterogeneous levels of hardseededness. Frozen seeds demonstrated higher germination percentages (the percentage of germinated seeds) and germination rates (time for first seed to germinate (T0) and time required (in days) to reach 50% of the final germination percentage (T50)) than the control ones. The anomalous seedlings were not observed after storage of seeds in liquid nitrogen. This study shows that cryostorage may be successfully applied for conservation of native species without detrimental effects on germination and growth.

show abstract

“…This procedure may prevent the ice crystals within the cell, which would lead to destruction of the membrane and consequently cause cell death (Goldfarb et al, 2010). Kholina & Voronkova (2008) using 103 species and Voronkova & Kholina (2010) using 11 species endemic to eastern Russia found that cryostored seeds subjected to slow thawing for 2 h, under laboratory environmental conditions (20−25 °C), had no loss or physiological damage. Thymus lotocephalus seeds stored in liquid nitrogen were slowly thawed at room temperature for approximately 18 h, and showed no signs of damage (Coelho et al, 2012).…”

Section: Dimethyl Sulfoxide Use In Cryopreservationmentioning

confidence: 99%

Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

et al. 2017

View full text Add to dashboard Cite

The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide.

show abstract

Conserving the gene pool of Far Eastern plants by means of seed cryopreservation

Cited by 11 publications

References 9 publications

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

Seed Cryopreservation of Some Medicinal Legumes

Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

Contact Info

Product

Resources

About