Conserved molecular interactions in centriole-to-centrosome conversion

Fu, Jingyan; Lipinszki, Zoltán; Rangone, Hélène; Min, Mingwei; Mykura, Charlotte; Chao-Chu, Jennifer; Schneider, Sandra; Dzhindzhev, Nikola S.; Gottardo, Marco; Riparbelli, Maria Giovanna; Callaini, Giuliano; Glover, David M.

doi:10.1038/ncb3274

Cited by 123 publications

(216 citation statements)

References 50 publications

(95 reference statements)

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“…2b–d). This orientation of Cep295 at centrioles is consistent with the Drosophila Ana-1 orientation as previously described26, supporting the notion that the function of Cep295 for centriole formation is evolutionarily conserved.…”

Section: Resultssupporting

confidence: 90%

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Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

et al. 2016

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Centrioles surrounded by pericentriolar material (PCM) serve as the core structure of the centrosome. A newly formed daughter centriole grows into a functional mother centriole. However, the underlying mechanisms remain poorly understood. Here we show that Cep295, an evolutionarily conserved protein, is required for generation of a bona fide mother centriole organizing a functional centrosome. We find that Cep295 is recruited to the proximal centriole wall in the early stages of procentriole assembly. Cep295 then acts as a scaffold for the proper assembly of the daughter centriole. We also find that Cep295 binds directly to and recruits Cep192 onto the daughter centriole wall, which presumably endows the function of the new mother centriole for PCM assembly, microtubule-organizing centre activity and the ability for centriole formation. These findings led us to propose that Cep295 acts upstream of the conserved pathway for centriole formation and promotes the daughter-to-mother centriole conversion.

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Section: Resultssupporting

confidence: 90%

“…In addition, it has recently been shown that the Cep135-Cep295/Ana1-Cep152/Asl interactions enable the centriole-to-centrosome conversion in both Drosophila melanogaster and humans26. In this study, we identify Cep295 as a novel conserved factor acting upstream of Cep192 in centriole biogenesis.…”

mentioning

confidence: 72%

Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole

et al. 2016

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“…For procentrioles, competence for duplication additionally requires the acquisition of PCM, a process termed ‘centriole to centrosome conversion’ 89,90 , which is also governed by CDK1 and PLK1 91,92 (Figure 2A). Best described are events in Drosophila , where PLK1 is first recruited to CPAP, through CDK1-dependent phosphorylation of a single docking site 92 .…”

Section: Control Of Centriole Numbermentioning

confidence: 99%

“…Best described are events in Drosophila , where PLK1 is first recruited to CPAP, through CDK1-dependent phosphorylation of a single docking site 92 . In both Drosophila and mammalian cells, PLK1 then triggers the sequential assembly of CEP135, CEP295/Ana1 and CEP152/Asl and downstream PCM formation 58,89,90 . Importantly, recruitment of Asl in Drosophila embryos only occurs after disengagement, indicating that these licensing processes occur sequentially 93 .…”

Section: Control Of Centriole Numbermentioning

confidence: 99%

Once and only once: mechanisms of centriole duplication and their deregulation in disease

Nigg

Holland

2018

Nat Rev Mol Cell Biol

390

546

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Centrioles are conserved microtubule-based organelles that form the core of the centrosome and act as templates for the formation of cilia and flagella. Centrioles have important roles in most microtubule related processes, including motility, cell division and cell signaling. To coordinate these diverse cellular processes, centriole number must be tightly controlled. In cycling cells, one new centriole is formed next to each preexisting centriole in every cell cycle. Advances in imaging, proteomics, structural biology and genome editing have revealed new insights into centriole biogenesis, how centriole numbers are controlled and how alterations in these structures contribute to diseases such as cancer and neurodevelopmental disorders. Moreover, recent work has uncovered the existence of surveillance pathways that limit proliferation of cells with numerical centriole aberrations. Here we discuss recent progress in this field with a focus on signaling pathways and molecular mechanisms.

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“…1 In a recent study, we demonstrated that centriole-to-centrosome conversion relies upon the building of a protein complex comprising Cep135, Ana1/Cep295 and Asl/Cep152 onto the nascent centriole in both Drosophila melanogaster and human cells. 4 Understanding centriole to centrosome conversion requires knowledge of the centriolar components that are loaded during mitosis, how they are organized in space and the dependencies of their recruitment. We first utilized 3D structured illumination super-resolution microscopy (3D-SIM) on cultured Drosophila cells to establish a timeline of the recruitment of proteins that are critical for centriole duplication, which had been identified from previous genome-wide screens.…”

mentioning

confidence: 99%