Conserved microtubule–actin interactions in cell movement and morphogenesis

Rodriguez, Olga; Schaefer, A.; Mandato, Craig A.; Forscher, Paul; Bement, William M.; Waterman‐Storer, Clare M.

doi:10.1038/ncb0703-599

Cited by 804 publications

(773 citation statements)

References 103 publications

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“…In this scenario, it is likely that the action of HDAC6 in angiogenesis is independent of gene transcription but instead involves cytoskeleton-associated events. This is supported by our finding that HDAC6 stimulates membrane ruffling at the leading edge of vascular endothelial cells and promotes cell polarization and migration, which are important steps in angiogenesis and rely on dynamic rearrangement of actin filaments and microtubules (Rodriguez et al, 2003;Lamalice et al, 2007).…”

Section: Discussionsupporting

confidence: 69%

“…For example, the centrosome is randomly localized in quiescent endothelial cells and reorients towards the leading edge in the presence of migratory stimuli (Bornens, 2008). Efficient cell migration also relies on dynamic rearrangement of the cytoskeleton (Rodriguez et al, 2003). Especially, a number of signaling molecules can associate with cytoske-letal components and modulate cytoskeletal dynamics, thereby promoting cell polarization and migration.…”

Section: Introductionmentioning

confidence: 99%

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Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner

Xie

Ren

et al. 2011

Protein Cell

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Angiogenesis, a process by which the preexisting blood vasculature gives rise to new capillary vessels, is associated with a variety of physiologic and pathologic conditions. However, the molecular mechanism underlying this important process remains poorly understood. Here we show that histone deacetylase 6 (HDAC6), a microtubule-associated enzyme critical for cell motility, contributes to angiogenesis by regulating the polarization and migration of vascular endothelial cells. Inhibition of HDAC6 activity impairs the formation of new blood vessels in chick embryos and in angioreactors implanted in mice. The requirement for HDAC6 in angiogenesis is corroborated in vitro by analysis of endothelial tube formation and capillary sprouting. Our data further show that HDAC6 stimulates membrane ruffling at the leading edge to promote cell polarization. In addition, microtubule end binding protein 1 (EB1) is important for HDAC6 to exert its activity towards the migration of endothelial cells and generation of capillary-like structures. These results thus identify HDAC6 as a novel player in the angiogenic process and offer novel insights into the molecular mechanism governing endothelial cell migration and angiogenesis.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner

Xie

Ren

et al. 2011

Protein Cell

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“…Thus, microtubule dynamics may activate Rho proteins, which in turn feed back to control the net growth and stability of microtubules at the leading edge [47]. The combined effects of Rho proteins on actin and microtubule cytoskeleton and the dynamic interactions [61,62] between these two filament systems indicate that Rho proteins exert their influence on cell motility by coordinate control of cytoskeleton converging on focal adhesion dynamics (Fig. 1B).…”

Section: Rho Regulation Of Microtubulesmentioning

confidence: 99%

Scaffold mediated regulation of MAPK signaling and cytoskeletal dynamics: A perspective

Pullikuth

Catling

2007

Cellular Signalling

136

107

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Cell migration is critical for many physiological processes and is often misregulated in developmental disorders and pathological conditions including cancer and neurodegeneration. MAPK signaling and the Rho family of proteins are known regulators of cell migration that exert their influence on cellular cytoskeleton during cell adhesion and migration. Here we review data supporting the view that localized ERK signaling mediated through recently identified scaffold proteins may regulate cell migration.

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“…Neurons represent an extreme example of cell asymmetry, and the cytoskeleton is directly involved in neurite extension (19,26). Cultured hippocampus embryonic murine neurons follow a classic pattern of development (23): Up to 20 hours, a few neurites appear (stage II), and most of them end in a growth cone.…”

Section: Effect Of Hmf On Neuronal Differentiationmentioning

confidence: 99%

“…Microtubules and actin filaments are of particular interest since both form dynamic networks that are constantly undergoing reorganization. There is a tight relationship between microtubules and actin spatial organizations on the one hand, and vital cellular functions, including cell division, cell motility, cell morphogenesis, and cell organization, on the other hand (19). In vitro, microtubule and actin polymer orientation is strongly affected by HMF above 5 T for microtubules (20) or above 12 T for actin filaments (21).…”

mentioning

confidence: 99%

Cellular disorders induced by high magnetic fields

Valiron

Peris

Rikken

et al. 2005

Magnetic Resonance Imaging

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Purpose: To evaluate whether static high magnetic fields (HMFs), in the range of 10 -17 T, affect the cytoskeleton and cell organization in different types of mammalian cells, including fibroblasts, epithelial cells, and differentiating neurons.Materials and Methods: Cells were exposed to HMF for 30 or 60 minutes and subsequently assessed for viability. Cytoskeleton arrays and focal adhesions were visualized using immunofluorescence microscopy.Results: Cell exposure to HMF over 10 T in the case of cycling cells, and over 15 T in the case of neurons, affected cell viability, apparently because of cell detachment from culture dishes. In the remaining adherent cells, the organization of actin assemblies was perturbed, and both cell adhesion and spreading were impaired. Moreover, in the case of neurons, exposure to HMF induced growth cone retraction and delayed cell differentiation. Conclusion:Cell exposure to HMF (over 10T and 15 T in the case of cycling cells and neurons, respectively) affects the cell cytoskeleton, with deleterious effects on cell viability, organization, and differentiation. Further studies are needed to determine whether such perturbations, as observed here in cultured cells, have consequences in whole animals.

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Conserved microtubule–actin interactions in cell movement and morphogenesis

Cited by 804 publications

References 103 publications

Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner

Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner

Scaffold mediated regulation of MAPK signaling and cytoskeletal dynamics: A perspective

Cellular disorders induced by high magnetic fields

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