Conserved Interaction of Lentiviral Vif Molecules with HIV-1 Gag and Differential Effects of Species-Specific Vif on Virus Production

Zheng, Wenwen; Ling, Limian; Li, Zhaolong; Wang, Hong; Rui, Yajuan; Gao, Wenying; Wang, Shaohua; Su, Xing; Wei, Wei; Yu, Xiao Fang

doi:10.1128/jvi.00064-17

Cited by 3 publications

(3 citation statements)

References 88 publications

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“…The DSB is repaired by nonhomologous end-joining (NHEJ) associated with InDel mutations at the cut site. tat, rev, vif, vpr, vpu, nef (Cavrois et al, 2017;Jakobsdottir et al, 2017;Joseph et al, 2014;Zheng et al, 2017) SIV NHP LYM, Mo, Mϕ, DC, MG 1° Receptor: CD4 Co-receptors: CCR5 or CXCR4 tat, rev, vif, vpr, vpu, vpx, nef (Merino et al, 2017;Micci et al, 2014;Sugimoto et al, 2017) FIV F, (NHP) LYM, Mo, MΦ, MG, Astro 1° Receptor: CD134 Co-receptor: CXCR4 rev, vif (Eckstrand et al, 2017;Meeker and Hudson, 2017;Meeker et al, 2012;Meltzer et al, 1990;Power, 2018) BIV B LYM, Mo, MΦ, FBLC, N, MG, BMEC CXCR5 suspected tat, rev, vif, vpw, vpy, tmx (Bhatia et al, 2013;Guo et al, 2013;Zhang et al, 1997Zhang et al, , 2014 SRLV (CAEV and MVV) O, C Mo, MΦ, DC, MG, BMEC, LYM, EC of MaGd Not yet identified; mannose receptor suspected rev, v...…”

Section: Discussionmentioning

confidence: 99%

Current and Future Therapeutic Strategies for Lentiviral Eradication from Macrophage Reservoirs

Peterson

MacLean

2018

J Neuroimmune Pharmacol

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Macrophages, one of the most abundant populations of leukocytes in the body, function as the first line of defense against pathogen invaders. Human Immunodeficiency virus 1 (HIV-1) remains to date one of the most extensively studied viral infections. Naturally occurring lentiviruses in domestic and primate species serve as valuable models to investigate lentiviral pathogenesis and novel therapeutics. Better understanding of the role macrophages play in HIV pathogenesis will aid in the advancement towards a cure. Even with current efficacy of first-and second-line Antiretroviral Therapy (ART) guidelines and future efficacy of Long Acting Slow Effective Release-ART (LASER-ART); ART alone does not lead to a cure. The major challenge of HIV eradication is viral latency. Latency Reversal Agents (LRAs) show promise as a possible means to eradicate HIV-1 from the body. It has become evident that complete eradication will need to include combinations of various effective therapeutic strategies such as LASER-ART, LRAs, and gene editing. Review of the current literature indicates the most promising HIV eradication strategy appears to be LASER-ART in conjunction with viral and receptor gene modifications via the CRISPR/Cas9 system.

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Section: Discussionmentioning

confidence: 99%

Current and Future Therapeutic Strategies for Lentiviral Eradication from Macrophage Reservoirs

Peterson

MacLean

2018

J Neuroimmune Pharmacol

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“…Відкрита рамка зчитування фактору інфекційності віріону Vif (virion infectivity factor) є консервативною для більшості лентівірусів. Молекули Vif приймають участь у реплікації вірусу за допомогою інактивування антивірусних факторів хазяїна, зокрема, цитидин деамінази APOBEC3 [22].…”

Section: структурний аналіз протеїнів відкритих рамок зчитування віру...unclassified

Structural analysis of open reading frames of bovine immunodeficiency virus proteins

Balak,

Lymanska

2023

Veterinary Medicine: Inter-Departmental Subject Scientific Collection

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The goal of this study was determining the structural organization peculiarities of the ORF2 and ORF3 proteins of the bovine immunodeficiency virus (BIV). Five ORFs were determined for two BIV isolates with complete genome using the ATGpr software, which permits effective prediction of translation initiation codons with nucleotide accuracy. Phyre2 software was used to predict, analyze the secondary structure and function of proteins. PONDR-FIT software was used to search for protein fragments in a disordered or natively unfolded state. Analysis of the amino acid composition of ORF2 and ORF3 proteins of BIVisolates regarding the presence of nonpolar, polar, aromatic, and hydrophobic amino acid residues was carried out using PSIPRED software. Models of the 3D-structure of proteins were obtained by I-TASSER server. 14% of α helices, 17% of β strands and 43% of disordered structure are predicted for the ORF3 protein. 37% of α helices, 0% of β strands, and 41% of disordered structure were determined for Gag polyprotein, which is translated from ORF2. The distribution of charged amino acid residues characterizes the surface properties of proteins. Their number reaches 23.9% for ORF2 protein. The amount of Arg is 5.2%, Lys — 8.0%, Glu — 7.3%, Asp — 3.4%. The total number of charged amino acid residues of ORF3 is 23.3%. The number of Arg is 12.6%, Lys — 4.9%, Glu — 1.9%, Asp — 3.9%. Only two ORFs of five ones coincide in nucleotide length (and, therefore, in length of corresponding proteins) for the two BIV isolates. The ORF3 protein belongs to the intrinsically disordered proteins that cannot be stably folded into a unique three-dimensional structure under physiological conditions, and the Gag polyprotein, which is translated from ORF2, belongs to the class of fully structured proteins. The secondary structure of both proteins shows the presence of α-helices

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“…To successfully replicate in host cells, viruses need to counteract various host restriction factors at different replication steps. It is evident from several reports that host restriction factors, such as apolipoprotein B mRNA-editing enzyme catalytic subunit 3 proteins (APOBEC3) [ 3 , 4 , 5 ], tripartite motif protein 5a (TRIM5a) [ 6 ], SAM domain and HD domain-containing protein 1 (SAMHD1) [ 7 , 8 ], and tetherin [ 9 ] play important roles in blocking interspecies transmission of retroviruses. As with several other restriction factors, like interferon-induced transmembrane proteins (IFITMs), tetherin has been shown to have broad antiviral activity against different enveloped viruses from various virus families including human immunodeficiency virus 1 (HIV-1), Ebola virus and human herpes virus 8 (HHV8) [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Strain-Specific Antagonism of the Human H1N1 Influenza A Virus against Equine Tetherin

Wang

Zhang

Wang

2018

Viruses

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Tetherin/BST-2/CD317 is an interferon-induced host restriction factor that can block the budding of enveloped viruses by tethering them to the cell surface. Many viruses use certain proteins to counteract restriction by tetherin from their natural hosts, but not from other species. The influenza A virus (FLUAV) has a wide range of subtypes with different host tropisms. Human tetherin (huTHN) has been reported to restrict only specific FLUAV strains and the viral hemagglutinin (HA) and neuraminidase (NA) genes determine the sensitivity to huTHN. Whether tetherins from other hosts can block human FLUAV is still unknown. Here, we evaluate the impact of equine tetherin (eqTHN) and huTHN on the replication of A/Sichuan/1/2009 (H1N1) and A/equine/Xinjiang/1/2007 (H3N8) strains. Our results show that eqTHN had higher restriction activity towards both viruses, and its shorter cytoplasmic tail contributed to that activity. We further demonstrated that HA and NA of A/Hamburg/4/2009 (H1N1) could counteract eqTHN. Notably, our results indicate that four amino acids, 13T and 49L of HA and 32T and 80V of NA, were involved in blocking the restriction activity of eqTHN. These findings reveal interspecies restriction by eqTHN towards FLUAV, and the role of the HA and NA proteins in overcoming this restriction.

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Conserved Interaction of Lentiviral Vif Molecules with HIV-1 Gag and Differential Effects of Species-Specific Vif on Virus Production

Cited by 3 publications

References 88 publications

Current and Future Therapeutic Strategies for Lentiviral Eradication from Macrophage Reservoirs

Current and Future Therapeutic Strategies for Lentiviral Eradication from Macrophage Reservoirs

Structural analysis of open reading frames of bovine immunodeficiency virus proteins

Strain-Specific Antagonism of the Human H1N1 Influenza A Virus against Equine Tetherin

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