Conserved chromosome segments in &lt;i&gt;Hylobates hoolock&lt;/i&gt; revealed by human and &lt;i&gt;H. leucogenys&lt;/i&gt; paint probes

Nie, Wei; Rens, W.; Wang, J.; Yang, Fengtang

doi:10.1159/000056912

Cited by 42 publications

(36 citation statements)

References 14 publications

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“…The previously available synteny arrangements in HLE and SSY were based on chromosome painting (Koehler et al 1995a,b;Nie et al 2001;Muller et al 2003). It is generally appreciated that BAC-FISH has a considerably higher resolution than chromosome painting.…”

Section: Novel Chromosomal Rearrangements In Hoolock and Symphalangusmentioning

confidence: 99%

A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons

Capozzi

Carbone²,

Stanyon

et al. 2012

Genome Res.

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Chromosome rearrangements in small apes are up to 20 times more frequent than in most mammals. Because of their complexity, the full extent of chromosome evolution in these hominoids is not yet fully documented. However, previous work with array painting, BAC-FISH, and selective sequencing in two of the four karyomorphs has shown that highresolution methods can precisely define chromosome breakpoints and map the complex flow of evolutionary chromosome rearrangements. Here we use these tools to precisely define the rearrangements that have occurred in the remaining two karyomorphs, genera Symphalangus (2n = 50) and Hoolock (2n = 38). This research provides the most comprehensive insight into the evolutionary origins of chromosome rearrangements involved in transforming small apes genome. Bioinformatics analyses of the human-gibbon synteny breakpoints revealed association with transposable elements and segmental duplications, providing some insight into the mechanisms that might have promoted rearrangements in small apes. In the near future, the comparison of gibbon genome sequences will provide novel insights to test hypotheses concerning the mechanisms of chromosome evolution. The precise definition of synteny block boundaries and orientation, chromosomal fusions, and centromere repositioning events presented here will facilitate genome sequence assembly for these close relatives of humans.

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Section: Novel Chromosomal Rearrangements In Hoolock and Symphalangusmentioning

confidence: 99%

A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons

Capozzi

Carbone²,

Stanyon

et al. 2012

Genome Res.

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“…However, it was proposed in a molecular analysis that these groups should be classified as 4 genera because the molecular distances between the 4 gibbon genera were in the same range as those between Homo and Pan, or even higher (Roos and Geissmann 2001). On the other hand, the gibbon has a very differentiated karyotype from humans (Jauch et al 1992, Koehler et al 1995, Nie et al 2001, Hirai et al 2003.…”

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confidence: 99%

A Discovery of Polymorphism of Nucleolar Organizer Regions (NORs) and Whole-Arm Translocation (WAT) between Chromosome 8 and 9 of Lowland Agile Gibbon (Hylobates agilis unko) in Thailand

et al. 2010

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Summary This paper succeeded in discovering the polymorphism of nucleolar organizer regions (NORs) and whole-arm translocation (WAT) between chromosomes 8 and 9 of the lowland agile gibbon (Hylobates agilis unko) in Thailand, after standard whole blood lymphocyte culture and Gbanding technique were applied to stain the chromosomes. The results showed that the 2n of H. agilis unko was 44. The type of autosomes were 28 metacentric, 12 submetacentric and 2 acrocentric chromosomes, with the X and Y-chromosome being submetacentric and acrocentric chromosome, respectively. In addition, a pair of the long arms of chromosome 12 showed clearly observable NORs. This is the first report on the polymorphism of NORs in H. agilis unko. The results show a heteromorphism in female with a difference size of NORs of chromosome 12, while in males show an equal size of both chromosome 12 with a homomorphism. We also detected a WAT between chromosomes 8 and 9 in H. agilis unko and found that both male and female chromosomes 8 and 9 were 8cЈ (homomorphism, 8cЈ8cЈ) and 9Ј (homomorphism, 9Ј9Ј), respectively, resulting from pericentric inversion and followed by reciprocal translocation. The family Hylobatidae (small apes) are included in Appendix I (threatened species) of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) as endangered species, together with great apes and other monkeys (Soehartono and Mardiastuti 2002). Gibbons (genus Hylobates) constitute a sister group to the great apes (Pongidae) and humans (Hominidae) (Groves 1972). The 4 genera of gibbons recognized by classical taxonomic criteria are further characterized by 4 unique karyotypes and diploid numbers (Chiarelli 1972, Markvong 1973, Prouty et al. 1983. Gibbons show a variable chromosome number ranging from 2n (diploid)ϭ38 to 2nϭ52, which provides a useful landmark to identify the genera of this family, Hylobates 2nϭ44, Hoolock 2nϭ38, Nomascus 2nϭ52 and Symphalangus 2nϭ50 (Prouty et al. 1983, Marshall and Sugardjito 1986, Geissmann 1995. Key wordsComparative cytogenetic studies reveal striking degrees of genomic rearrangement since divergence of the 4 groups from a common ancestor (Dutrillaux et al. 1975, Myers and Shafer 1979, Couturier et al. 1982, Prouty et al. 1983, Van Tuinen and Ledbetter 1983. However, it was * Corresponding author, e-mail: tanomtong@hotmail.com © 2010 The Japan Mendel Society Cytologia 75(1): [15][16][17][18][19][20][21] 2010

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“…The chromosome band number which generated by high-resolution technique of white-handed gibbon (Hylobates lar) (Jauch et al 1992) and black crested gibbon (Hylobates concolor) (Koehler et al 1995) are 574 and 420 bands, respectively. Whereas the G-banding show that the chromosome band number of hoolock gibbon (Hylobates hoolock) are 230 bands (Nie et al 2001).…”

Section: Resultsmentioning

confidence: 99%

Standardized Karyotype and Idiogram of the Pileated Gibbon, Hylobates pileatus (Primate, Hylobatidae) by G-banding and High-resolution Technique

2007

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Summary Standardized karyotype and idiogram of the pileated gibbon (Hylobates pileatus) Nakhon Ratchasima Zoo, Thailand was studied. Blood sample were taken from 2 females and 2 males. After standard whole blood lymphocyte culture at 37°C for 72 h in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of pileated gibbon was 2n (diploid)ϭ44. The type of autosomes were 30 metacentric, 10 submetacentric and 2 acrocentric chromosomes, with X and Y chromosome being submetacentric and acrocentric chromosome, respectively. From the G-banding and high-resolution technique, the numbers of bands and locations in the pileated gibbon were 183 and 236 respectively, each chromosome pair could be clearly differentiated.

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Conserved chromosome segments in <i>Hylobates hoolock</i> revealed by human and <i>H. leucogenys</i> paint probes

Cited by 42 publications

References 14 publications

A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons

A comprehensive molecular cytogenetic analysis of chromosome rearrangements in gibbons

A Discovery of Polymorphism of Nucleolar Organizer Regions (NORs) and Whole-Arm Translocation (WAT) between Chromosome 8 and 9 of Lowland Agile Gibbon (Hylobates agilis unko) in Thailand

Standardized Karyotype and Idiogram of the Pileated Gibbon, Hylobates pileatus (Primate, Hylobatidae) by G-banding and High-resolution Technique

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