Conservative chemical modification of proteins to study the effects of a single protein property on partitioning in aqueous two-phase systems

Franco, T. T.; Andrews, Anthony T.; Asenjo, Juan A.

doi:10.1002/(sici)1097-0290(19960205)49:3<290::aid-bit7>3.0.co;2-f

Cited by 31 publications

(17 citation statements)

References 13 publications

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“…The purification factor was 5,000. A similar tendency were observed by Franco et al [10] using the ATPS in the batch mode. They obtained a BSA partition coefficient of around 0.003.…”

Section: Selectivitysupporting

confidence: 87%

“…The tie line for this system was constructed according to Snyder et al [9]. Samples of ATPS (10 g each) were prepared in duplicate in test tubes [10], and phase separation was achieved by centrifugation for 5 min at 3,000 g. Approximately 500 mg of each phase was weighed in a glass tube and two volumes of water were added. A freeze-dryer was used to sublimate the water for 24 h, and the tubes were weighed again.…”

Section: Preparation Of Atps and Determination Of The Tie Linementioning

confidence: 99%

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Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Igarashi

Kieckbusch

Franco

2004

Bioprocess and Biosystems Engineering

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Aqueous two-phase systems (ATPSs) have long been used for biomolecule partitioning; these systems offer the possibility of using continuous or semicontinuous extraction processes. They require relatively simple equipment like spray or sieve plate columns that can be adapted for use in ATPSs. The aim of this work was to study the semicontinuous extraction of a model enzyme, xylanase, in spray and sieve plate columns, since, unlike centrifugal contactors, the cost of construction and maintenance of this equipment is low and it is easy to operate. For the spray column, the dispersed phase hold-up and overall mass transfer coefficients K(D) a were evaluated for different column heights and for different superficial velocities of the dispersed phase (light phase). Results indicated that an increase in superficial velocity in the range of 0-0.18 mm/s of the dispersed phase had a positive effect on K(D) a and on hold-up in all column heights studied, 75, 161 and 246 mm. For the same superficial velocity of the dispersed phase, the larger the hold-up was, the shorter the column. For the sieve plate column, the effects of the superficial velocity of the dispersed phase and the number of plates were also studied. Results showed that the K(D) a and hold-up increased with an increase in both parameters. The selectivity of separation of xylanase and BSA (model contaminant) was very high, since 60% of the enzyme was extracted in the light phase, whereas no significant amount of BSA was extracted. The possibility of using the sieve plate column in continuous operation for enzyme extraction was studied because previous work had only addressed the semicontinuous extraction of enzyme. The residence time distribution of the PEG phase using different superficial velocities of the salt phase was studied in continuous operation. The time required to reach the steady state was 40 min, and 70% of the xylanase was recovered. It was found that the Modified Power Spline software was well adjusted to the experimental results.

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“…The purification factor was 5,000. A similar tendency were observed by Franco et al [10] using the ATPS in the batch mode. They obtained a BSA partition coefficient of around 0.003.…”

Section: Selectivitysupporting

confidence: 87%

Section: Preparation Of Atps and Determination Of The Tie Linementioning

confidence: 99%

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Igarashi

Kieckbusch

Franco

2004

Bioprocess and Biosystems Engineering

Self Cite

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“…In general, the selection of salts for PEG-salt ATPS is depending on their salting-out ability of promoting hydrophobic interactions between biomolecules [67,68]. For instance, PEG-phosphate ATPS have been widely used for the recovery of bioproducts [5,67].…”

Section: Factors Affecting Protein Partitioningmentioning

confidence: 99%

Evaluation of Driving Forces for Protein Partition in PEG-Salt Aqueous Two- Phase Systems and Optimization by Design of Experiments

Glyk¹,

Solle²,

Scheper³

et al. 2016

J Chromatogr Sep Tech

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“…The selection of salts for the ATPS depends on their ability to promote hydrophobic interactions between biomolecules [64]. The PEG/phosphate system is widely used for recovery of bioproducts [65].…”

Section: Impact Of the Type Of Saltsmentioning

confidence: 99%

Aqueous Two-Phase Extraction Advances for Bioseparation

Goja¹,

Yang

Cui³

et al. 2013

J Bioproces Biotechniq

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Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer-polymer type and polymer-salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the high value and achieve good control over processes, the reliable, multi-component products are required especially those with the ability to investigate complex processing conditions. The current reviewing paper discusses the most recent progresses for the recovery of biomolecules by using the ATPE, covering the mechanism, which controls the phase formation and the behavior of solute partitioning in aqueous twophase systems (ATPS) processes. The review discusses also the increasing application for the recovery of high-value bioproducts, the recent development of alternative low cost ATPS and disadvantages attributed to ATPS.

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Conservative chemical modification of proteins to study the effects of a single protein property on partitioning in aqueous two-phase systems

Cited by 31 publications

References 13 publications

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Xylanase mass transfer studies in aqueous two-phase systems using spray and sieve plate columns

Evaluation of Driving Forces for Protein Partition in PEG-Salt Aqueous Two- Phase Systems and Optimization by Design of Experiments

Aqueous Two-Phase Extraction Advances for Bioseparation

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