Conservation Status of Globally Testudines Terrapins Based on COI Mitochondrial Markers

Salleh, Mohd Hairul Mohd; Esa, Yuzine

doi:10.3390/mol2net-08-12456

Cited by 2 publications

(7 citation statements)

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“…Based on the IUCN Red List of the 12 species of terrapins, 25% were critically endangered (CR) and 8% were endangered (EN). The terrapins studied all inhabit fresh or brackish water [26]. Furthermore, "terrapin" refers to more or less aquatic, hard-shelled turtles [60].…”

Section: Discussionmentioning

confidence: 99%

“…Notably, refs. [3,26] identified 13 terrapin species worldwide but ignored a previously thoughtto-be-extinct Seychelles black terrapin species (Pelusios seychellensis). However, a genetic analysis of the lectotype revealed that this terrapin is not extinct and is now known as Pelusios castaneus.…”

Section: Discussionmentioning

confidence: 99%

“…Batagur affinis ssp. is found only on the west coast of Peninsular Malaysia and is extinct in Sumatra, Indonesia [25,26].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, the subspecies B. a. edwardmolli, located on the east coast of Peninsular Malaysia that once reached from Singapore to Southeast Asia, is now thought to have vanished from Vietnam, Thailand, Singapore, and Indonesia [23,26,27]. Currently, only Peninsular Malaysia and Cambodia are home to this species [18,23,24,28].…”

Section: Introductionmentioning

confidence: 99%

“…The samples are limited due to the conservation status of B. affinis ssp., which has been listed as critically endangered on the IUCN Red List since 2000 [16]. This study compares them to the other eleven terrapin species listed by [3,26] and accessed from the public database portal.…”

Section: Introductionmentioning

confidence: 99%

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Global Terrapin Character-Based DNA Barcodes: Assessment of the Mitochondrial COI Gene and Conservation Status Revealed a Putative Cryptic Species

Salleh

Esa

Mohamed

2023

Animals

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Technological and analytical advances to study evolutionary biology, ecology, and conservation of the Southern River Terrapin (Batagur affinis ssp.) are realised through molecular approaches, including DNA barcoding. We evaluated the use of COI DNA barcodes in Malaysia’s Southern River Terrapin population to better understand the species’ genetic divergence and other genetic characteristics. We evaluated 26 sequences, including four from field specimens of Southern River Terrapins obtained in Bota Kanan, Perak, Malaysia, and Kuala Berang, Terengganu, Malaysia, as well as 22 sequences from global terrapins previously included in the Barcode of Life Database (BOLD) Systems and GenBank. The species are divided into three families: eight Geoemydidae species (18%), three Emydidae species (6%), and one Pelomedusidae species (2%). The IUCN Red List assigned the 12 species of terrapins sampled for this study to the classifications of critically endangered (CR) for 25% of the samples and endangered (EN) for 8% of the samples. With new haplotypes from the world’s terrapins, 16 haplotypes were found. The intraspecific distance values between the COI gene sequences were calculated using the K2P model, which indicated a potential cryptic species between the Northern River Terrapin (Batagur baska) and Southern River Terrapin (Batagur affinis affinis). The Bayesian analysis of the phylogenetic tree also showed both species in the same lineage. The BLASTn search resulted in 100% of the same species of B. affinis as B. baska. The Jalview alignment visualised almost identical sequences between both species. The Southern River Terrapin (B. affinis affinis) from the west coast of Peninsular Malaysia was found to share the same haplotype (Hap_1) as the Northern River Terrapin from India. However, B. affinis edwardmolli from the east coast of Peninsular Malaysia formed Hap_16. The COI analysis found new haplotypes and showed that DNA barcodes are an excellent way to measure the diversity of a population.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Batagur affinis ssp. is found only on the west coast of Peninsular Malaysia and is extinct in Sumatra, Indonesia [25,26].…”

Section: Introductionmentioning

confidence: 99%