Conservation of Valuable Gene Resources by Cryopreservation in some Forest Tree Species

Jörgensen, Jörg

doi:10.1016/s0176-1617(11)80065-9

Cited by 32 publications

(11 citation statements)

References 4 publications

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“…After thawing, the pollen germination did not change and even exceeded that of non-cryopreserved pollen (Jörgensen 1990). Ryynänen (1999a) stored birch buds collected from hardened shoots in LN and shoots were regenerated from the buds after thawing (Ryynänen 1999b;Ryynänen and Häggman 1999).…”

Section: Introductionmentioning

confidence: 94%

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Chmielarz

2009

Acta Physiol Plant

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To evaluate the feasibility of long-term cryopreservation of Polish provenances of silver birch (Betula pendula), the sensitivity of conditionally dormant seeds to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; -196°C) was evaluated. The critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or for 2 years in LN was also determined. Germination tests revealed no critical WC for seeds [to 0.02 g H 2 O g -1 dry mass (g g -1 )]. Seeds tolerated freezing in LN within specific safe range of WC 0.02-0.23 g g -1 (nuts). Seeds desiccated to the safe WC and stored in LN for 2 years had similar or higher germination as seeds stored at -3°C for 2 years, depending on provenance. Therefore, long-term cryopreservation of B. pendula seeds in gene banks is feasible.

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Section: Introductionmentioning

confidence: 94%

Cryopreservation of conditionally dormant orthodox seeds of Betula pendula

Chmielarz

2009

Acta Physiol Plant

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“…(Ford et al 2000), Larix 9 eurolepis (Klimaszewska et al 1992), and Abies nordmanniana (Nørgaard et al 1993). Within the context of forestry practices, cryopreservation programs for the ET of selected spruce and pine species have been widely implemented in Canada, France, New Zealand, Sweden, and the USA (Cyr 1999;Jorgensen 1990;Klimaszewska et al 1992;Park et al 1998;Park 2002). Different plant material often require different conditions to ensure a successful cyropreservation process.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce)

Hazubska-Przybył

Chmielarz

Michalak

et al. 2010

Plant Cell Tiss Organ Cult

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Two-year-old embryogenic tissues (ET) of Picea omorika (Pančić) Purk. were successfully cryopreserved after preculture with sucrose, air-drying for 2 h, and freezing in liquid nitrogen (LN). The preculture protocol consisted of passaging the ET onto standard Litvay medium containing increasing concentrations of sucrose (0.25 M sucrose for 24 h, 0.5 M for 24 h, 0.75 M for 2 days, and 1.0 M for 3 days) for 7 days, at 25°C, and in the dark. The clumps were subsequently air-dried over silica gel, down to a 20% water content (based on fresh weight), placed in cryovials, and immersed in liquid nitrogen (LN) for 24 h. These were thawed at 42°C and progressively rehydrated in phytagel-solidified LM media containing decreasing concentrations of sucrose. After 3 weeks of in vitro culture, surviving clumps were friable and white in color, similar to their morphology prior to cryostorage. The frequency of bacterial and fungal contamination was higher if ET was frozen in LN-containing vials than in LN-free vials. This efficient cryopreservation protocol would be useful for the ex-situ conservation of P. omorika germplasm in gene banks at very low temperatures.

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“…Shoots derived form subcultured shoot tips and nodal segments had a low rooting and survival rate (21 %) after 4 months. 56% of shoots derived from subcultured basal segments with a callus, rooted (Chalupa, 1984(Chalupa, , 1988 (Vieitez et al, 1985;Evers et al, 1987;San José et al, 1988, 1990Juncker and Favre, 1989) as well as plants in soil (Meier-Dinkel, 1987;Chalupa, 1988) (Jörgensen, 1990 …”

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confidence: 99%