Conservation of the putative methyltransferase domain: a hallmark of the 'Sindbis-like' supergroup of positive-strand RNA viruses

Rozanov, Mikhail; Koonin, Eugene V.; Gorbalenya, Alexander E.

doi:10.1099/0022-1317-73-8-2129

Cited by 350 publications

(242 citation statements)

References 22 publications

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“…RNA1 and RNA2 encodes for the protein 1a and 2a, respectively which forms the replicase complex [31]. N-terminal region of 1a protein contains putative methyltransferase domain [39] and C-terminal region shows sequence similarity to viral helicases [16]. RNA 2 encodes for another protein 2b, which is expressed from the subgenomic RNA 4A and acts as suppressor of gene silencing [4,5,27]; plays a role in long distance movement of the virus [9,23,43,54] and also behave as pathogenicity determinant [10,41,43].…”

Section: Introductionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India.

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Section: Introductionmentioning

confidence: 99%

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

et al. 2013

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“…Starting from the Nterminal region (aa 66-242), the putative methyltransferase domain of the 'Sindbis-like' supergroup of positive-strand RNA viruses was identified with the four domains described by Rozanov et al (1992). Typical motifs of nucleic acid helicases (Gorbalenya & Koonin, 1989) were located in the central part (aa 920-1140) of this polypeptide, whereas the GDD motif of RNA-dependent RNA polymerase (Koonin, 1991) was in the C-terminal region between aa 1390-1621.…”

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The nucleotide sequence and genomic organization of grapevine virus B

Saldarelli¹,

Minafra²,

Martelli³

1996

Journal of General Virology

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“…The 1a protein deduced sequence includes an N-proximal putative methyltransferase domain, involved in the capping of genomic and subgenomic RNAs, and a C-proximal putative helicase (superfamily 1) domain [17]. The PSV-Mi ORF2a (nt 82-2620 of RNA2) is predicted to encode a 2a protein of 846 aa's with a calculated M r of 94,647.…”

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Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China

Liu

Goldbach

et al. 2005

Arch Virol

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[12,21]. PSV is an economically important threat to peanut production in China. Severe epidemics of virus diseases caused by PSV along with Peanut stripe virus (PStV) and Cucumber mosaic virus (CMV) have substantially reduced peanut yields in the major peanut growing areas in northern China since the 1970 s [22]. In addition, disease surveys revealed that black locust trees (Robinia pseudoacacia L.), a popular tree in northern China, are widely infected by PSVThe nucleotide sequence data of RNA1, 2 and 3 of PSV-Mi have been deposited in GenBank under accession numbers AY429431, AY429430 and AY775057.

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Conservation of the putative methyltransferase domain: a hallmark of the 'Sindbis-like' supergroup of positive-strand RNA viruses

Cited by 350 publications

References 22 publications

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber

The nucleotide sequence and genomic organization of grapevine virus B

Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China

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