Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.

Michel, François; Dujon, Bernard

doi:10.1002/j.1460-2075.1983.tb01376.x

Cited by 500 publications

(244 citation statements)

References 28 publications

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“…Although RF3 is related to the IB family of intron open reading frames (22), the RF3 optional region is probably not an intron sequence for it does not retain the two highly conserved oligonucleotide motifs GACUA and AUAGUC (35). In this respect RF3 resembles RFI and RF2, which also belong to multigenic transcription units (5) and are located immediately downstream of non-mosaic genes coding for the respiratory proteins.…”

Section: Discussionmentioning

confidence: 99%

A mitochondrial reading frame which may code for a maturase-like protein inSaccharomyces cerevisiae

1985

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In S. cerevisiae, the large oxi3/oli2 mitochondrial transcript contains the products of the oxi3, a and oli2 genes and an unassigned reading frame, RF3. In the work presented here, we have completed the nucleotide sequence of RF3. We have shown that RF3 is composed of four fairly large ORFs which overlap within GC rich sequences. Furthermore, a shift of +1 base was found between each pair of consecutive reading frames. We discuss how these frameshifts could be removed to produce a 500 aminoacid long protein containing the two well conserved P1 and P2 oligopeptide sequences featuring several mitochondrial intron reading frames, suggesting, thereby, a RNA-maturase-like activity for the putative RF3 protein. In addition, we suggest that the insertion of GC clusters in a gene could provide a novel way of regulating its expression. INTRODUCTIONIn S. cerevisiae, the whole span of mitochondrial DNA coding for oxi3

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Section: Discussionmentioning

confidence: 99%

A mitochondrial reading frame which may code for a maturase-like protein inSaccharomyces cerevisiae

1985

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“…Chloroplast genes can be interrupted by introns, which are classified into the main groups I and II, based on primary and secondary structural features (Michel and Dujon 1983). Although some of these organellar introns have the capacity to self-splice in vitro, it is likely that these splicing processes are assisted by protein factors in vivo similar to the snRNP-mediated splicing events in the nuclear cell compartment.…”

Section: Rbps Involved In Splicing or Editing Of Chloroplast Transcriptsmentioning

confidence: 99%

Chloroplast RNA-binding proteins

Nickelsen

2003

Current Genetics

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Chloroplast gene expression is regulated by nucleus-encoded factors, which mainly act at the posttranscriptional level. Plastid RNA-binding proteins (RBPs) represent good candidates for mediating these functions. The picture emerging from recent analyses is that of a great number of differentially regulated RBPs, which are organized in distinct, spatially separated supramolecular complexes. This reflects the complexity of the regulatory network that underlies the intracellular communication system between the nucleus and the chloroplast.

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“…The trnT-trnF region of the chloroplast genome consists of one group I intron, one intergenic spacer, and one trnT exon. The group I intron is a well-conserved region encoding the self-splicing region of pre-tRNA (Michel and Dujon 1983;Cech 1988). In this study, we tried to find polymorphism from seven species or sub-species of pines using the intergenic spacer between trnT(UGU) and trnL(UAA) of chloroplast DNA.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Pines Based on Chloroplast trnT-trnL Intergenic Spacer DNA Sequences

Um¹,

Park²,

Jo³

et al. 2014

Journal of Forest and Environmental Science

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This study was conducted to distinguish the pines that are too similar to differentiate using conventional methods.Pinus densiflora and Pinus sylvestris have similar anatomical structure. They both have window-like pits and dentate ray tracheids, so it is not easy to distinguish the plants. We tried to find molecular markers by comparing chloroplast DNA sequences to differentiate the pines growing in Korea. We used P. densiflora, P. densiflora for. multicaulis, P. sylvestris, P. rigida, P. rigitaeda, P. koraiensis, and P. bungeana for this study. We found that the non-coding intergenic region of trnT(UGU) and trnL(UAA) genes have differences among the species. We designed a primer set to amplify the region efficiently and compared the PCR product sequences using CLC Workbench programs to find the polymorphism. We could distinguish the species using the sequences of the amplified region and the sequences were reproducible from the pines collected in Korea.

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Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.

Cited by 500 publications

References 28 publications

A mitochondrial reading frame which may code for a maturase-like protein inSaccharomyces cerevisiae

A mitochondrial reading frame which may code for a maturase-like protein inSaccharomyces cerevisiae

Chloroplast RNA-binding proteins

Phylogenetic Analysis of Pines Based on Chloroplast trnT-trnL Intergenic Spacer DNA Sequences

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