Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms

Huh, T.Y.; Gray, Michael W.

doi:10.1007/bf00021035

Cited by 40 publications

(14 citation statements)

References 10 publications

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“…Analysis of restriction patterns has revealed extensive variation in the structure of m t D N A from some closely related plant species [e.g., 47], and comparison of available gene maps [9,26,53] indicates that there is virtually no conservation of gene arrangement in plant mtDNA, with the exception of a close linkage of 18S and 5S rRNA genes [18]. In these respects, the plant mitochondrial genome is quite unlike its counterpart in vertebrate animals, which is small, shows little size variation, diverges in sequence at a relatively rapid rate within coding sequences, and has maintained an invariant gene order throughout 500 million years of chordate evolution [1,6,46].…”

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confidence: 99%

Multiple sequence rearrangements accompanying the duplication of a tRNAPro gene in wheat mitochondrial DNA

1988

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In the course of isolating tRNA genes from wheat mtDNA, we have found the same tRNA(Pro) gene in two different Hind III restriction fragments, H-P1 (0.7 kbp) and H-P2 (1.7 kbp). Sequences immediately flanking these duplicate genes are closely related, although not identical; sequence comparisons suggest that multiple rearrangements have occurred in the vicinity of the H-P2 tRNA(Pro) gene, relative to the H-P1 version. The chimeric nature of H-P2 is emphasized by the presence of sequences that are also found upstream of the wheat mitochondrial 26S rRNA gene, as well as sequences derived from chloroplast DNA. Comparison of H-P2 with H-P1 plus upstream sequences provides some insight into possible molecular events that might have generated H-P2. In particular, such comparisons suggest a model in which the homologous sequences in H-P2 are seen to be derived from H-P1 plus upstream sequences as a result of an intragenomic, site-specific rearrangement event, followed by amplification of the product, its fixation in the mitochondrial genome, and subsequent sequence divergence (single base changes as well as insertions/deletions of up to 50 nucleotides). The results reported here implicate particular primary sequence motifs in certain of the rearrangements that characterize H-P2.

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confidence: 99%

Multiple sequence rearrangements accompanying the duplication of a tRNAPro gene in wheat mitochondrial DNA

1988

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“…The plant mitochondrial rrn5 is located just downstream of and in the same transcriptional orientation as rns, the small subunit rRNA gene (Chao et al, 1983;Gray & Spencer, 1983;Brennicke et al, 1985;Gwynn et al, 1987). This close physical linkage between rns and rrn5 (Bonen & Gray, 1980) is conserved among angiosperm mtDNAs (Huh & Gray, 1982).…”

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A 5 S rRNA Gene is Present in the Mitochondrial Genome of the ProtistReclinomonas americanabut is Absent from Red Algal Mitochondrial DNA

Lang

Goff

Gray

1996

Journal of Molecular Biology

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“…In plant mitochondria, rRNA genes are larger and a 5S RNA is found in mitochondrial ribosomes (6,15). The 5S and 18S rRNA genes in plant mtDNA are closely linked (3,10,12), but both are separate from the 26S rRNA gene (24). In maize, the 26S rRNA gene is separated from the 18S and 5S by 16,000 base pairs (bp) of DNA (24).…”

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Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial Genome

Chao¹,

Sederoff²,

Levings³

1983

Plant Physiol.

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The nucleotide sequence has been determined for a 664-base pair region of maize (Zea mays L.) mtDNA which contains the 3' end of the 18S rRNA gene, the 5S rRNA gene, and an intergenic region of 108 base pairs. Specific regions of the 18S rRNA gene show striking homology with the corresponding gene in Escherichia coli.The organization and structure of the rRNA genes in plant mitochondria are different from those of animals and fungi. In plant mitochondria, rRNA genes are larger and a 5S RNA is found in mitochondrial ribosomes (6, 15). The 5S and 18S rRNA genes in plant mtDNA are closely linked (3, 10, 12), but both are separate from the 26S rRNA gene (24). In maize, the 26S rRNA gene is separated from the 18S and 5S by 16,000 base pairs (bp) of DNA (24). Each of the rRNA genes is encoded by a single gene (24).This organization of 18S and 5S rRNA genes is unique to plant mtDNA. Prokaryotes have clusters of 16S, 23S, and 5S genes in that order. Chloroplasts usually have an organization like prokaryotes. Most eukaryotic nuclei have clusters of 5S genes which are unlinked to the 18S and 28S rRNA genes (17). To study the structure of plant mitochondrial rRNA genes, we have isolated a cloned fragment which contains the linked genes of 18S and 5S rRNA and determined a portion of the nucleotide sequence.The section of the molecule which has been sequenced contains part of the 18S rRNA gene and a complete 5S rRNA gene. We have located the 5S gene by sequence homology using the published sequence of the 5S rRNA from the mitochondria of wheat (22). Similarly, the location of the 3' end of the 18S gene was found because of strong homology with the corresponding genes of E. coli and maize chloroplast. The location of these boundary sequences defines the intergenic region which is 8 bp long and establishes the orientation and direction of transcription of both 18S and 5S rRNA genes.The large degree of homology found between the mitochondrial sequence and the bacterial sequence provides further support for the view that plant mitochondria have an endosymbiotic-eubacterial origin (2, 9, 10).

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Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms

Abstract: In six different angiosperms, mitochondrial genes for 18S and 5S rRNAs were found to be closely linked but distant from mitochondrial 26S rRNA genes.

Cited by 40 publications

References 10 publications

Multiple sequence rearrangements accompanying the duplication of a tRNAPro gene in wheat mitochondrial DNA

Multiple sequence rearrangements accompanying the duplication of a tRNAPro gene in wheat mitochondrial DNA

A 5 S rRNA Gene is Present in the Mitochondrial Genome of the ProtistReclinomonas americanabut is Absent from Red Algal Mitochondrial DNA

Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of the Maize Mitochondrial Genome

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