Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species

Ishida, Yuichi; Mabuchi, Yo; Naraoka, Yuna; Hisamatsu, Daisuke; Akazawa, Chihiro

doi:10.3390/ijms241511908

Cited by 3 publications

(2 citation statements)

References 24 publications

(41 reference statements)

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“…Disparities in adipogenesis between beef cattle's intramuscular and subcutaneous adipose progenitors have been documented [24,25]. The study of bovine adipose stem and progenitor cells revealed that preadipocytes can be collected and characterized as CD26−CD146+CD54+ or CD26−CD146+CD54− [26]. Song et al reported that porcine ADSCs could be differentiated into adipocytes using insulin, dexamethasone, IBMX, and rosiglitazone [27].…”

Section: Introductionmentioning

confidence: 99%

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Naraoka,

Mabuchi,

Kiuchi

et al. 2024

Cells

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The demand for stem cell-based cultured meat as an alternative protein source is increasing in response to global food scarcity. However, the definition of quality controls, including appropriate growth factors and cell characteristics, remains incomplete. Cluster of differentiation (CD) 29 is ubiquitously expressed in bovine muscle tissue and is a marker of progenitor cells in cultured meat. However, CD29+ cells are naturally heterogeneous, and this quality control issue must be resolved. In this study, the aim was to identify the subpopulation of the CD29+ cell population with potential utility in cultured meat production. The CD29+ cell population exhibited heterogeneity, discernible through the CD44 and CD344 markers. CD29+CD44−CD344− cells displayed the ability for long-term culture, demonstrating high adipogenic potential and substantial lipid droplet accumulation, even within 3D cultures. Conversely, CD29+CD44+ cells exhibited rapid proliferation but were not viable for prolonged culture. Using cells suitable for adipocyte and muscle differentiation, we successfully designed meat buds, especially those rich in fat. Collectively, the identification and comprehension of distinct cell populations within bovine tissues contribute to quality control predictions in meat production. They also aid in establishing a stable and reliable cultured meat production technique.

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Section: Introductionmentioning

confidence: 99%

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Naraoka,

Mabuchi,

Kiuchi

et al. 2024

Cells

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“…Pulmonary toxicity is a recognized adverse effect of anticancers, nevertheless, a mortality rate of 1%–4% from bleomycin is considered unacceptable for patients with treatable malignancies. The toxic effects of bleomycin are primarily caused by the generation of free radical and its lung specificity caused by bleomycin catalyzing hydrolase, which was not found in lung tissue, rendering this organ vulnerable to bleomycin toxicity ( Usman et al, 2010 ; Ishida et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Capsaicin ameliorate pulmonary fibrosis via antioxidant Nrf-2/ PPAR- γ pathway activation and inflammatory TGF-β1/ NF-κB/COX II pathway inhibition

Abdulaal,

Asfour,

Helmi

et al. 2024

Front. Pharmacol.

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Bleomycin is an effective antibiotic with a significant anticancer properties, but its use is limited due to its potential to induce dose-dependent pulmonary fibrosis. Therefore, this study aimed to assess the therapeutic potential of Capsaicin as an additional treatment to enhance patient tolerance to Bleomycin compared to the antifibrotic drug Pirfenidone. Pulmonary fibrosis was induced in rats through by a single intratracheal Bleomycin administration in day zero, followed by either Capsaicin or Pirfenidone treatment for 7 days. After the animals were sacrificed, their lungs were dissected and examined using various stains for macroscopic and histopathological evaluation. Additionally, the study assessed various antioxidant, anti-inflammatory, and antifibrotic parameters were assessed. Rats exposed to Bleomycin exhibited visible signs of fibrosis, histopathological alterations, increased collagen deposition, and elevated mucin content. Bleomycin also led to heightened increased inflammatory cells infiltration in the bronchoalveolar lavage, elevated fibrosis biomarkers such as hydroxyproline, alpha-smooth muscle actin (α-SMA) and transforming growth factor-beta (TGF-β1), increased inflammatory markers including tumor necrosis factor-alpha (TNF-α), interlukine-6 (Il-6), interlukine-1β (Il-1β) nuclear factor-kappa B (NF-κB), and Cyclooxygenase-2 (COX-2), and transforming growth factor-beta (TGF-β1),. Furthermore, it reduced the expression of peroxisome proliferator-activated receptor-gamma (PPAR-γ), increased oxidative stress biomarkers like nitric oxide (NO), malondialdehyde (MDA), myeloperoxidase (MPO) and protein carbonyl. Bleomycin also decreased the expression of nuclear factor erythroid 2–related factor 2 (Nrf-2), reduced glutathione (GSH), total antioxidant capacity, and the activities of catalase and superoxide dismutase (SOD). Treating the animals with Capsaicin and Pirfenidone following Bleomycin exposure resulted in improved lung macroscopic and microscopic characteristics, reduced collagen deposition (collagen I and collagen III) and mucin content, decreased inflammatory cell infiltration, lowered levels of hydroxyproline, α-SMA, and TGF-β1, decreased TNF-α, Il-6, Il-1β, NF-κB, and COX-2, increased PPAR-γ and Nrf-2 expression, and improvement improved in all oxidative stress biomarkers. In summary, Capsaicin demonstrates significant antifibrotic activity against Bleomycin-induced lung injury that may be attributed, at least in part, to the antioxidant and anti-inflammatory activities of Capsaicin mediated by upregulation of PPAR-γ and Nrf-2 expression and decreasing. TGF-β1, NF-κB and COX II proteins concentrations.

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Review: Livestock cell types with myogenic differentiation potential: Considerations for the development of cultured meat

Olenic,

Deelkens,

Heyman

et al. 2024

animal

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Conservation of Markers and Stemness in Adipose Stem and Progenitor Cells between Cattle and Other Species

Cited by 3 publications

References 24 publications

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Capsaicin ameliorate pulmonary fibrosis via antioxidant Nrf-2/ PPAR- γ pathway activation and inflammatory TGF-β1/ NF-κB/COX II pathway inhibition

Review: Livestock cell types with myogenic differentiation potential: Considerations for the development of cultured meat

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