Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes

Borruso, Luigimaria; Salomone‐Stagni, Marco; Polsinelli, Ivan; Schmitt, Armin O.; Benini, Stefano

doi:10.1007/s00203-017-1409-7

Cited by 18 publications

(18 citation statements)

References 71 publications

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“…It can be seen that some species in the three genera Sphingomonas, Curtobacterium and Buttiauxella have certain significance for the study of drought resistance of upland rice. The two genera, Aurantimonas and Erwinia, have also been identified in plants (Liu et al 2016;Li et al 2018;Borruso et al 2017). Some species of Aurantimonas have certain antibacterial activity, and researchers have previously isolated an Aurantimonas aggregata sp.…”

Section: The Venn Diagram Data (Supplementarymentioning

confidence: 99%

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High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice

et al. 2020

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Upland rice is an ecotype crop formed by long-term domestication and evolution of rice in the dry land without water layer. Generally, its stem and leaf are thick and luxuriant, its leaf is wide and light, its root system is developed, its root hair is abundant, its osmotic pressure of root and cell juice concentration of leaf are high, and it is drought resistant, heat-resistant and water absorbing. The purpose of this study is to reveal the "core flora" of endophytes in upland rice seeds by studying the diversity and community structure of endophytes in upland rice seeds, and to reveal the impact of soil environment on the formation of endophyte community structure in upland rice seeds by comparing with soil environment microorganisms in upland rice habitats. In this study, the high-throughput sequencing technology based on the Illumina Hiseq 2500 platform was used to study the structure and diversity of endophytic bacterial communities using upland rice varieties collected in different places and soil samples from their unified planting sites as materials. There are 42 endophytic OTUs coexisted in the 14 samples. At the phylum level, the first dominant phyla was Proteobacteria (93.81-99.99%) in all 14 samples. At the genus level, Pantoea (8.77%-87.77%), Pseudomonas (1.15-61.58%), Methylobacterium (0.40-4.64%), Sphingomonas (0.26-3.85%), Microbacterium (0.01-4.67%) and Aurantimonas (0.04-4.34%), which are probably the core microflora in upland rice seeds, served as the dominant genera that coexisted in all upland rice seeds tested. Compared with the soil microbial community structure in the upland rice uniform planting site, it was found that it had little effect on the endophytic community structure in upland rice seeds. This study is of great significance for the isolation, screening, functional evaluation and reaction of some functional microorganisms in upland rice in order to improve its agronomic traits. It also provides a certain reference for the interaction between microorganisms and plants. the adjacent plant tissues. Therefore, endophytes are ubiquitous in the plant host, including the aboveground and underground parts of the plant, even seeds, thus having a positive impact on the plant development (Zinniel et al. 2015; Chebotar et al. 2015). Endophytes can provide a variety of benefits to host plants, especially through multiple functions to improve the growth and health of plants, so that they can be protected from pathogens (Agler et al. 2016; Liu et al. 2017a,b; Xu et al. 2019; Afzal et al. 2019; Sánchez-Cruz et al. 2019). Under different environmental conditions, the communication and interaction between endophyte and plant is stronger than that between rhizosphere bacteria (Coutinho et al. 2015). A number of studies have shown that plant varieties, genotypes and geographical location have a great impact on the establishment of microbial diversity and community structure in plants (Liu et al. 2017a, 2019; Edwards et al. 2015). Although endophyte was discovered more than 100 years ago, its existe...

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Section: The Venn Diagram Data (Supplementarymentioning

confidence: 99%

“…nov. in extreme environment (Pereira et al 2017;Li et al 2017). Erwinia amylovora often lead to the fire blight disease, which causes huge economic losses to apples and pears (Borruso et al 2017).…”

Section: The Venn Diagram Data (Supplementarymentioning

confidence: 99%

High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice

et al. 2020

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“…Mutations in the sorbitol operon of E. amylovora impair sorbitol utilization in apple shoots, thus preventing efficient colonization of host plant tissue (Aldridge et al 1997). It has been proposed that, differences in host specificity between the Spiraeoideae infecting strains and the Rubus infecting strains might be due to the lack of a complete sorbitol operon in the Rubus infecting strains (Borruso et al 2017). For a better understanding of SrlD mechanism of action its activity and 3D structure were determined together with structural and sequence comparisons (Salomone-Stagni et al 2018a).…”

Section: Srldmentioning

confidence: 99%

“…In order to suppress the host organism's defenses, E. amylovora secretes several proteins into the host cytoplasm via the Type 3 Secretion System (T3SS) (Vrancken et al 2013;Borruso et al 2017). The cysteine protease AvrRpt2 is one of the type III effector proteins which are secreted by E. amylovora (AvrRpt2 EA ) (Zhao et al 2006;Khan et al 2012;Schropfer et al 2018) AvrRpt2 is activated by peptidyl-prolyl cis/trans isomerization after secretion inside the host cell by T3SS (Coaker et al 2006).…”

Section: Avrrpt2mentioning

confidence: 99%

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Structural and functional characterization of proteins from the fire blight pathogen Erwinia amylovora. A review on the state of the art

Benini

2020

J Plant Pathol

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Together with genome analysis and knock-out mutants, structural and functional characterization of proteins provide valuable hints on the biology of the organism under investigation. Structural characterization can be achieved by techniques such as X-ray crystallography, NMR, Cryo-EM. The information derived from the structure are a good starting point to comprehend the details of the proteins molecular function for a better understanding of their biological role. This review aims at describing the progress in the structural and functional characterization of proteins from the plant pathogen Erwinia amylovora obtained by structural biology and currently deposited in the Protein Data Bank.

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Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora

Polsinelli¹,

Caliandro²,

Salomone‐Stagni³

et al. 2019

International Journal of Biological Macromolecules

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Conservation of Erwinia amylovora pathogenicity-relevant genes among Erwinia genomes

Cited by 18 publications

References 71 publications

High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice

High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice

Structural and functional characterization of proteins from the fire blight pathogen Erwinia amylovora. A review on the state of the art

Comparison of the Levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora

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