Conservation of epitopes in the oligosaccharide portion of the lipooligosaccharide of Haemophilus influenzae type b

Abstract

The antigenic characteristics of the lipooligosaccharide (LOS) of Haemophilus influenzae type b (Hib) were examined in strains obtained over an extended period of time. These Hib strains were isolated from patients with systemic Hib disease in Dallas, Tex., over a 20-year period and in New York City between 1941 and 1956. The antigenic characteristics of the LOS of these Hib strains were examined by using a set of four murine monoclonal antibodies directed against epitopes present in the oligosaccharide portion of the LOS molecule. The same basic set of LOS antigenic determinants that is expressed by recent Hib isolates was also found to be present in this collection of Hib strains spanning a 40-year period. Some variation with time was detected in the distribution of the systemic disease isolates among four Hib LOS antigenic groups; however, only 2 of 188 Hib isolates failed to react with a set of two LOS-specific monoclonal antibodies. Therefore, little variation has occurred among Hib strains with regard to the LOS epitopes defined by these monoclonal antibodies over a considerable period of time.

Tumor necrosis factor alpha/cachectin and interleukin 1 beta initiate meningeal inflammation.

“…Hib strain DL42 is a non-0-lactamase-producing strain isolated from the CSF of a child with meningitis . This strain has been shown to be virulent in the infant rat model for Hib invasive disease and to belong to LOS antigenic group 2, which is the predominate LOS antigenic type among invasive Hib strains (40) . rrIL1 S. rrID10 was purified from the extract of Escherichia coli (HB 101) that was transformed with a rabbit 11,10-containing plasmid.…”

Use of the complete genome sequence information of Haemophilus influenzae strain Rd to investigate lipopolysaccharide biosynthesis

“…For kdtA, orf18 (kdtB ) and nusG, no transformants were obtained. Analysis of LPS from mutant strains by immunoblotting LPS molecules from wild-type and mutant strains were analysed by colony immunoblotting using a panel of monoclonal antibodies (mAbs), each specific for particular LPS epitopes (Gulig et al, 1987;Borrelli et al, 1995;Borrelli, 1996). Table 2 shows the reactivities of mAbs 4C4, 6A2, 12D9, 5G8 and 7E7 to the wild-type strain RM7004 and mutant derivatives.…”

The role of a repetitive DNA motif (5′‐CAAT‐3′) in the variable expression of the Haemophilus influenzae lipopolysaccharide epitope αGal(1–4)βGal

“…Three H. influenzae type b strains were used in this study: H. influenzae strain RM7004 or 760705 {van Alphen et al. 1983); H. influenzae strain Eagan (Anderson ef al.. 1972) and H. influenzae DL42 (Gulig et al. 1987).…”

“…mAb 4C4 recognizes a surface-exposed epitope in the Iipopoiysaccharide of H. influenzae strain DL42 (Gulig et at.. 1987). This epitope has the structure aGal(1-4.…”

“…)pGal (Virji et al, 1989). mAb 5G8 recognizes a different surface-exposed lipopolysaccharide epitope from DL42 (Gulig et at., 1987). Monoclonal A1-12E-5G-8E (Al) was kindly provided by A. Lindberg.…”