Conservation of Endangered Wild Harvested Medicinal Plants: Use of DNA Barcoding

Veldman, Sarina; Otieno, Joseph; Gravendeel, Barbara; Andel, Tinde van; Boer, Hugo de

doi:10.1002/9781118460566.ch6

Cited by 20 publications

(23 citation statements)

References 71 publications

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“…Similar to carnivores, DNA barcoding of the diet of herbivores is also challenging, as barcoding of plants cannot be accomplished using a single DNA region only. Often, a combination of two barcode regions is needed (Veldman et al 2014;Srivathsan et al 2015). Even with all precautions as described above taken into account, some components of mammalian diets will still fail to be retrieved using a DNA approach.…”

Section: Diet Analysismentioning

confidence: 99%

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

2016

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. (2016). Added value of metabarcoding combined with microscopy for evolutionary studies of mammals. -Zoologica Scripta, 45, 37-49. Metabarcoding -taxon identification from complex mixtures using a standard DNA region -is increasingly used in evolutionary studies. With this method, it is not only possible to delimit species or collect indirect observational data but also to determine diets. Caveats such as false negatives and skewed abundances can be overcome when metabarcoding is combined with traditional methods such as microscopy. Such a combined approach can help deducing why some species went extinct or became endangered whereas others evolved into new lineages. This review will focus on the added value of metabarcoding when combined with traditional methods for evolutionary studies of mammals.

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Section: Diet Analysismentioning

confidence: 99%

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

2016

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“…Terrestrial orchids have been used for medicinal and culinary purposes for centuries [1], with the most notable example being the use of orchid tubers to make salep, a traditional Turkish creamy drink or ice cream, consumed in Asia Minor and several countries on the Balkan peninsula [2][3][4][5]. In south-eastern Africa terrestrial orchid tubers are mixed with peanut flour, salt, baking soda and chili powder to make a traditional Zambian meat-like cake known as chikanda or African polony [6][7][8]. Although initially not highly regarded [9], chikanda has more recently become popular throughout the country.…”

Section: Introductionmentioning

confidence: 99%

“…It is sold as a snack along the streets, on markets, in supermarkets and on the menu of high-end restaurants [10] and recipes as well as cooking tutorial videos can be found online [11]. Orchids used for chikanda are harvested exclusively from the wild, and although it is unlikely that traditional village consumption poses a serious threat to orchid populations, the increased popularity and subsequent commercialization of chikanda has led to the exhaustion of Zambian orchid resources [8]. Collecting tubers means the end of a perennial and generally longlived orchid, since the entire plant is removed in the harvesting process.…”

Section: Introductionmentioning

confidence: 99%

Trade in Zambian Edible Orchids – DNA Barcoding Reveals Use of Unexpected Orchid Taxa for <em>Chikanda</em>

Veldman¹,

Kim²,

Andel³

et al. 2018

Preprint

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In Zambia wild edible terrestrial orchids are used to produce a local delicacy called chikanda, which has become increasingly popular throughout the country. Commercialization puts orchid populations in Zambia and neighbouring countries at risk of overharvesting. Hitherto, no study has documented which orchid species are traded on local markets, as orchid tubers are difficult to identify morphologically. In this study, the core land-plant DNA barcoding markers rbcL and matK were used in combination with nrITS to determine which species were sold on Zambian markets. Eighty-two interviews were conducted to determine harvesting areas, as well as possible sustainability concerns. By using nrITS DNA barcoding, a total of 16 orchid species in six different genera could be identified. Both rbcL and matK proved suitable to identify the tubers up to genus- or family level. Disa robusta, Platycoryne crocea and Satyrium buchananii were identified most frequently and three previously undocumented species were encountered on the market. Few orchid species are currently listed on the global IUCN Red List. Local orchid populations and endemic species could be at risk of overharvesting due to the intensive and indiscriminate harvesting of chikanda orchids and we therefore encourage increased conservation assessment  of terrestrial African orchids.

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“…Plant misidentification may be due to similarities in appearance during bulk purchase or when harvested (Boullata and Nace, 2000;Liu et al, 2015). Confusing nomenclature or terms with common, transliterated, Latin, and scientific names could also lead to misidentification (Newmaster et al, 2013;Chen et al, 2014;Veldman et al, 2014;Wu et al, 2015). In addition to safety concerns, the quality of herbal products has also received increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

Xiong

Liu

et al. 2016

Front. Plant Sci.

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Correct identification of medicinal plant ingredients is essential for their safe use and for the regulation of herbal drug supply chain. Loop-mediated isothermal amplification (LAMP) is a recently developed approach to identify herbal medicine species. This novel molecular biology technique enables timely and accurate testing, especially in settings where infrastructures to support polymerase chain reaction facilities are lacking. Studies that used this method have altered our view on the extent and complexity of herbal medicine identification. In this review, we give an introduction into LAMP analysis, covers the basic principles and important aspects in the development of LAMP analysis method. Then we presented a critical review of the application of LAMP-based methods in detecting and identifying raw medicinal plant materials and their processed products. We also provide a practical standard operating procedure (SOP) for the utilization of the LAMP protocol in herbal authentication, and consider the prospects of LAMP technology in the future developments of herbal medicine identification and the challenges associated with its application.

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Conservation of Endangered Wild Harvested Medicinal Plants: Use of DNA Barcoding

Cited by 20 publications

References 71 publications

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

Trade in Zambian Edible Orchids – DNA Barcoding Reveals Use of Unexpected Orchid Taxa for <em>Chikanda</em>

Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

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Conservation of Endangered Wild Harvested Medicinal Plants: Use of DNA Barcoding

Cited by 20 publications

References 71 publications

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

Added value of metabarcoding combined with microscopy for evolutionary studies of mammals

Trade in Zambian Edible Orchids &ndash; DNA Barcoding Reveals Use of Unexpected Orchid Taxa for <em>Chikanda</em>

Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification

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Trade in Zambian Edible Orchids – DNA Barcoding Reveals Use of Unexpected Orchid Taxa for <em>Chikanda</em>