Conservation linkages of endangered medicinal plant and exploration of phytochemicals, pharmaceutical screening and in silico validation against diabetics using in vivo wild and in vitro regenerated plant Boucerosia diffusa Wight.

Vignesh, Arumugam; Amal, Thomas Cheeran; Sree, S. Jaya; Selvakumar, S.; Vasanth, Krishnan

doi:10.1007/s13205-023-03645-5

Cited by 6 publications

(1 citation statement)

References 43 publications

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“…In our study, we investigated the ability of C. tuberculata callus extract to suppress the enzymatic function of alpha-amylase, thereby reducing the hydrolysis of polysaccharides and subsequently limiting the release of glucose into the bloodstream. 53 The antidiabetic metabolites present in the callus extract bind to the enzyme’s catalytic site, preventing substrate binding and the initiation of catalytic activity. This leads to a reduction in the breakdown of the sugar compounds.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Hypoglycemic Potential of Phytogenic Selenium Nanoparticle- and Light Regime-Mediated In Vitro Caralluma tuberculata Callus Culture Extract

Ali,

Mashwani,

Raja

et al. 2024

ACS Omega

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In vitro plant cultures have emerged as a viable source, holding auspicious reservoirs for medicinal applications. This study aims to delineate the antioxidant and hypoglycemic potential of phytosynthesized selenium nanoparticle (SeNP)-and light stress-mediated in vitro callus cultures of Caralluma tuberculata extract. The morphophysicochemical characteristics of biogenic SeNPs were assessed through a combination of analytical techniques, including UV−visible spectrophotometry, scanning electron microscopy, energy-dispersive X-rays, Fourier transform infrared spectrometry, and zeta potential spectroscopy. The antioxidative potential of the callus extract 200 and 800 μg/mL concentrations was assessed through various tests and exhibited pronounced scavenging potential in reducing power (26.29%), ABTS + scavenging (42.51%), hydrogen peroxide inhibition (37.26%), hydroxyl radical scavenging (40.23%), and phosphomolybdate (71.66%), respectively. To inspect the hypoglycemic capacity of the callus extract, various assays consistently demonstrated a dosage-dependent relationship, with higher concentrations of the callus extract exerting a potent inhibitory impact on the catalytic sites of the alpha-amylase (78.24%), alpha-glucosidase (71.55%), antisucrase (59.24%), and antilipase (74.26%) enzyme activities, glucose uptake by yeast cells at 5, 10, and 25 mmol/L glucose solution (72.18, 60.58 and 69.33%), and glucose adsorption capacity at 5, 10, and 25 mmol/L glucose solution (74.37, 83.55, and 86.49%), respectively. The findings of this study propose selenium NPs and light-stress-mediated in vitro callus cultures of C. tuberculata potentially operating as competitive inhibitors. The outcomes of the study were exceptional and hold promising implications for future medicinal applications.

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Section: Resultsmentioning

confidence: 99%