Conservation Genetics of Neotropical Pollinators Revisited: Microsatellite Analysis Suggests That Diploid Males Are Rare in Orchid Bees

Souza, Rogério Oliveira de; Lama, Marco Antônio Del; Cervini, Marcelo; Mortari, Norma; Eltz, Thomas; Zimmermann, Yvonne; Bach, Carola; Brosi, Berry J.; Suni, Sevan S.; Quezada-Euán, J. J. G.; Paxton, Robert J.

doi:10.1111/j.1558-5646.2010.01052.x

Cited by 31 publications

(43 citation statements)

References 71 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on their findings, Souza et al (2010) suggested that diploid males are rare in orchid bees. However, it should be noticed that about 50 % of the species surveyed by Souza et al (2010) were represented by a restricted number of individuals (n<20) in the samples analysed. Thus, further studies involving larger samples would be interesting to confirm whether diploid males are in fact rare among orchid bees and if this hypothesis can be generalized for the group.…”

Section: Introductionmentioning

confidence: 99%

“…Both of these studies were based on allozyme analyses (Roubik et al 1996;Zayed et al 2004), as was a study carried out by Takahashi et al (2001), which found very different results. More recently, Souza et al (2010), using microsatellite markers, analysed 1010 euglossine males, belonging to 27 different species across the Neotropics, and also detected very low frequencies of 2N males for all samples studied. Based on their findings, Souza et al (2010) suggested that diploid males are rare in orchid bees.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, Souza et al (2010), using microsatellite markers, analysed 1010 euglossine males, belonging to 27 different species across the Neotropics, and also detected very low frequencies of 2N males for all samples studied. Based on their findings, Souza et al (2010) suggested that diploid males are rare in orchid bees. However, it should be noticed that about 50 % of the species surveyed by Souza et al (2010) were represented by a restricted number of individuals (n<20) in the samples analysed.…”

Section: Introductionmentioning

confidence: 99%

“…Considering that declines in bee populations could result in genetic diversity loss (Cook 1993;Zayed 2009) and that in euglossine bees, the sl-CSD system has been proposed as the sexdetermining mechanism for different species of this group of bees (Zayed et al 2004;van Wilgenburg et al 2006;Souza et al 2010), it would be expected that in small populations of these bees, a higher frequency of 2N males should occur (Zayed et al 2004;Zayed 2009). Despite this, to date, information regarding diploid male frequency in forest fragment showing different sizes and/or different degrees of disturbance is still scarce in the literature.…”

Section: Introductionmentioning

confidence: 99%

“…In the last two decades, several studies have investigated the frequency of diploid males in Euglossini (Roubik et al 1996;Zayed et al 2004;López-Uribe et al 2007;Souza et al 2010), a Neotropical group of bees popularly known as orchid bees considered to be important pollinators throughout the tropical Americas (Dressler 1982;Roubik and Hanson 2004). Roubik et al (1996) reported high frequencies of these males in populations of some species of Euglossini (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

et al. 2015

View full text Add to dashboard Cite

-The incidence of diploid males among 1457 individuals of different populations of six euglossine bee species (Eufriesea violacea , Eulaema cingulata , Euglossa annectans , Euglossa iopoecila , Euglossa pleosticta and Euglossa truncata ) was investigated with microsatellite markers. Bees were surveyed in Atlantic Forest fragments showing different sizes and degrees of human interference. Our analyses revealed that, although the frequencies of diploid males remained below 10 % for five out of six species studied, the frequencies of these males in three populations of Eg. annectans were above 15.0 %. For this species, while the average estimate of diploid males was around 11.0 %, the highest percentage (27 %) of such males was found for the population from an island. Our findings indicate that the frequency of diploid males can vary substantially among species and this fact should be taking into consideration in studies and conservation actions involving different euglossine bees. microsatellites / Euglossini / euglossine bees / Euglossa annectans / forest fragments

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

et al. 2015

View full text Add to dashboard Cite

show abstract

Diploid male production correlates with genetic diversity in the parasitoid wasp Venturia canescens: a genetic approach with new microsatellite markers

Collet

Vayssade

Auguste

et al. 2016

Ecology and Evolution

View full text Add to dashboard Cite

Sex determination is ruled by haplodiploidy in Hymenoptera, with haploid males arising from unfertilized eggs and diploid females from fertilized eggs. However, diploid males with null fitness are produced under complementary sex determination (CSD), when individuals are homozygous for this locus. Diploid males are expected to be more frequent in genetically eroded populations (such as islands and captive populations), as genetic diversity at the csd locus should be low. However, only a few studies have focused on the relation between population size, genetic diversity, and the proportion of diploid males in the field. Here, we developed new microsatellite markers in order to assess and compare genetic diversity and diploid male proportion (DMP) in populations from three distinct habitat types – mainland, island, or captive –, in the parasitoid wasp Venturia canescens. Eroded genetic diversity and higher DMP were found in island and captive populations, and habitat type had large effect on genetic diversity. Therefore, DMP reflects the decreasing genetic diversity in small and isolated populations. Thus, Hymenopteran populations can be at high extinction risk due to habitat destruction or fragmentation.

show abstract

Simple method for combining multiple-loci marker genotypes to estimate diploid male proportion, with an application to a threatened bumble bee population in Japan

Nomura

Taniguchi²

2022

Insect. Soc.

View full text Add to dashboard Cite

Conservation Genetics of Neotropical Pollinators Revisited: Microsatellite Analysis Suggests That Diploid Males Are Rare in Orchid Bees

Cited by 31 publications

References 71 publications

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

Orchid bees: a new assessment on the rarity of diploid males in populations of this group of Neotropical pollinators

Diploid male production correlates with genetic diversity in the parasitoid wasp Venturia canescens: a genetic approach with new microsatellite markers

Simple method for combining multiple-loci marker genotypes to estimate diploid male proportion, with an application to a threatened bumble bee population in Japan

Contact Info

Product

Resources

About