Conservation and variation of ribosomal DNA in eukaryotes

Kupriyanova, N. S.

doi:10.1007/bf02759600

Cited by 14 publications

(23 citation statements)

References 87 publications

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“…By sequencing this rDNA region, the authors demonstrated that the difference in IGS size is due to the different number of subrepeated elements. IGS length may vary in different individuals of the same species, and even within the genome of the same individual (Kupriyanova 2000). In G. lutea, we revealed intergenomic polymorphism of rDNA repeats varied in size.…”

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 88%

“…It is believed that this is due to the binding of transcription factors, whose amount is limited, to predominantly longer IGS containing more enhancer subrepeats. The deficit of transcription factors leads to inactivation of the nucleolus of one parent because of chromatin condensation, which in its turn is due to differential methylation of cytosine and acetylation of histones, as was demonstrated for wheat-rye hybrids (Kupriyanova 2000).…”

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 90%

“…For example, in interspecific hybrids of grasses, rDNA with the longer IGS dominates over rDNA with a shorter spacer (Kupriyanova 2000;Volkov et al 2007). It is believed that this is due to the binding of transcription factors, whose amount is limited, to predominantly longer IGS containing more enhancer subrepeats.…”

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 97%

“…Recently, it has been demonstrated that the number of some subrepeats in the upstream region might produce an effect on the intensity of expression of rRNA genes in plants (Kupriyanova 2000;Volkov et al 2007). For example, in interspecific hybrids of grasses, rDNA with the longer IGS dominates over rDNA with a shorter spacer (Kupriyanova 2000;Volkov et al 2007).…”

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 98%

“…IGS may contain several kinds of short repeated elements, each 20-300 bp in length, also referred to as subrepeats. IGS is the most dynamic region of the rDNA with several internal subrepeats in its sequence, which evolve rapidly both in size and structure (Paule and Lofquist 1996;Komarnytsky and Komarnytsky 2000;Kupriyanova 2000;Volkov et al 2003a).…”

Section: Variability Of Nuclear Ribosomal Rna Genesmentioning

confidence: 99%

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Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Кунах

Mel’nyk

Дробык

et al. 2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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This chapter presents the results of cytogenetic and molecular genetic studies on tissue and organ cultures from some Gentiana species. Cytogenetic analysis of calli from seven gentians showed that all of them (except for G. punctata) consisted mainly of the cells with diploid and/or near-diploid chromosome sets with low anaphase aberration. Species specificity of cytogenetic structure was established for cell populations in vitro. An increase in the proportion of polyploid cells was found for G. acaulis callus after long-term culture. RAPD analysis of tissue cultures from six gentians showed that culture resulted in genetic changes, with the species differing in the level of genetic variation. RAPD and ISSR analyses of G. pneumonanthe and G. lutea genetic variation in tissue and organ cultures and regenerated plants revealed the most significant changes in isolated root cultures as well as in long-term cultured tissues. At the same time, direct and indirect regenerants had the least genetic difference compared to donor plant. The restriction maps of nuclear 18S-25S ribosomal DNA from G. acaulis, G. punctata, G. lutea, and G. asclepiadea were constructed based on the results of blot hybridization. The decrease of 18S-25S rDNA amount was found in cultured tissues. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, such as the appearance of repeats with altered length. A relationship has been suggested between the peculiarities of the structural organization of the 18S-25S rRNA gene cluster and its rearrangements. Rearrangements of 5S rDNA were not found in cultures of Gentiana L. species, except for the variation in nucleotide methylation pattern at restriction sites.

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Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 88%

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 90%

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 97%

Section: Rflp Analysis Of Interspecific Polymorphism Of Ribosomal Dnamentioning

confidence: 98%

Section: Variability Of Nuclear Ribosomal Rna Genesmentioning

confidence: 99%

See 3 more Smart Citations

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Кунах

Mel’nyk

Дробык

et al. 2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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The Gentianaceae - Volume 2: Biotechnology and Applications

Rybczyński¹,

Davey²,

Mikuła³

2015

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Nucleotide variation and physical mapping of ribosomal genes using FISH in genus Tor (Pisces, Cyprinidae)

et al. 2010

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Molecular cytogenetic studies were carried out for localization of 18S and 5S ribosomal DNAs on chromosomes of three cyprinid fish species viz., T. khudree, T. mussullah and T. mosal mahanadicus using two color fluorescence in situ hybridization (FISH). All the species typically possessed 100 diploid chromosomes with minor variation in karyo-morphology. The 18S rDNA signals were observed on two pair of chromosomes in T. khudree and T. mussullah, and three pairs in T. mosal mahanadicus. The location of 18S signals also showed affinity to silver nitrate and chromomycin A3 staining. Similarly, variation in localization of 5S rDNA among the three species has been detected with the presence of FISH signals on one pair of chromosome in T. khudree and T. mussullah, and on two pairs in T. mosal mahanadicus. These molecular markers could be used as species specific markers for taxonomic identification and can further add in understanding the dynamics of genome organization and karyotypic evolution of these species. The 18S rDNA region was sequenced that generated 1811, 1810 and 1776 bp long 18S sequence in T. khudree, T. mussullah and T. mosal mahanadicus, respectively. The 18S rDNA sequence showed 95-98% identity among the subject species. Similarly, 5S sequencing generated 203 bp long fragments in these species with 100% identity in coding and 9.63% variability in non-transcribed spacer regions. The nucleotide sequence variations could be used for understanding the genetic diversity and will add new informative characters in comparative genomics. These results, in general, would enhance the value and interpretation of ecological assessment data for conservation of Tor species.

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Conservation and variation of ribosomal DNA in eukaryotes

Cited by 14 publications

References 87 publications

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

The Gentianaceae - Volume 2: Biotechnology and Applications

Nucleotide variation and physical mapping of ribosomal genes using FISH in genus Tor (Pisces, Cyprinidae)

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