Conservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer aerietinum L.) within the genus Cicer

Choumane, Wafaa; Winter, Peter; Weigand, F.; Kahl, Günter

doi:10.1007/s001220051479

Cited by 88 publications

(67 citation statements)

References 35 publications

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“…Chickpea being narrow in genetic base, most of the regions are highly conserved which results in such low polymorphism. The results from the present study were in agreement with the observations of several workers about the potential utility of STMS in characterizing chickpea germplasm (Bharadwaj et al, 2010(Bharadwaj et al, , 2011Huttel, 1999, Flandez-galvez, 2003and Choumane, 2000.…”

Section: Cultivar-specific Markerssupporting

confidence: 93%

Genetic diversity analysis among selected short duration chickpeacultivars and breeding lines based on STMS markers.

Koinain¹,

Hegde²,

Bharadwaj³

2016

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Genetic diversity among 30 chickpea genotypes was evaluated using simple sequence repeat (SSR) molecular markers. The studies using Sequence Tagged Microsatellite Site (STMS) markers markers revealed that among the primers used across the genotypes produced a total of 35 alleles representing 21 SSR loci with frequencies ranging from one to two (mean 1.66) alleles per locus. Polymorphic Information Content (PIC) ranged from 0.098 to 0.500 (CAM0443, CAM0446). These primers might be an effective and useful tool to determine the genetic differences among chickpea genotypes and to study the phylogenetic relationships. Polymorphic percentage was 96.42. Hierarchical neighbour-joining UPGMA cluster analysis based on simple matching similarity matrix resolved the 30 genotypes into seven clusters. Based on STMS markers highest similarity index 0.850 was observed between BGD 72 and Annigeri-1whereas BGD 9920 and ICC 92944 showed the lowest similarity index 0.214 between them. The STMS clustering pattern indicated the presence of wide genetic diversity between the genotypes. Overall, the study ascertained that SSRs provide powerful marker tools in revealing genetic diversity and relationships in chickpeas, thereby proving useful for selection of parents in breeding programs and also for DNA fingerprinting for identification of cultivars.

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Section: Cultivar-specific Markerssupporting

confidence: 93%

Genetic diversity analysis among selected short duration chickpeacultivars and breeding lines based on STMS markers.

Koinain¹,

Hegde²,

Bharadwaj³

2016

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“…The flanking sequences of microsatellite loci of cultivated chickpea were found to be conserved in related annual species also (Choumane et al, 2000;Winter et al, 1999). The highest degree of conservation was observed in C. reticulatum (92 per cent) and C. echinospermum (83 per cent), whereas C. cuneatum showed the lowest (50 per cent) (Choumane et al, 2000), which further supports the crossability, isozyme and RAPD studies based grouping of species. C. anatolicum, a wild perennial relative of chickpea, showed 72 per cent conservation, which strongly supports the karyotypic studies (Ahmad, 1989).…”

Section: Molecular Studiessupporting

confidence: 73%

Chickpea Improvement: Role of Wild Species and Genetic Markers

Singh

Sharma

Varshney

et al. 2008

Biotechnology and Genetic Engineering Reviews

109

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Chickpea is an important grain legume of the semi arid tropics and warm temperate zones, and forms one of the major components of human diet. However, a narrow genetic base of cultivated chickpea (Cicer arietinum L.) has hindered the progress in realizing high yield gains in breeding programs. Furthermore, various abiotic and biotic stresses are the major bottlenecks for increasing chickpea productivity. Systematic collection and evaluation of wild species for useful traits has revealed presence of a diverse gene pool for tolerance to the biotic and abiotic stresses. Relationships among the species of genus Cicer are presented based on crossability, karyotype and molecular markers. The reproductive barriers encountered during interspecific hybridization are also examined. Recent information on genetic linkage maps, comparison of isozymes

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“…Amplification with the same set of primers should be possible only if these species are closely related. Several studies have used homologous microsatellite flanking sequences developed in one species for diversity analysis in closely related species (Udupa et al 1999, Choumane et al 2000, Brondani et al 2003, Adonina et al 2005.…”

Section: Isolation Of Microsatellitesmentioning

confidence: 99%

Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils

et al. 2009

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This paper reports the development of new microsatellite markers for lentil (Lens culinaris subsp. culinaris) and their use for genetic diversity analysis of a lentil core collection developed at ICARDA (Aleppo-Syria). Fourteen new markers were developed from microsatellite flanking sequences of a genomic library from a cultivated lentil accession ILL5588. The core collection used comprises 109 accessions from 15 countries representing 57 cultigens (including 18 breeding lines) from 8 countries and 52 wild types of germplasm (L. culinaris subsp. orientalis, L. culinaris subsp. tomentosus and L. culinaris subsp. odemensis) from 11 countries. Total number of alleles detected across all microsatellite loci was 182, with a mean of 13 alleles per locus. The wild accessions were rich in alleles (151 alleles) compared to cultigens (114 alleles). The genetic diversity index for the microsatellite loci in the wild accessions ranged from 0.16 (for locus SSR28 in L. culinaris subsp. odemensis) to 0.93 (for locus SSR66 in L. culinaris subsp. orientalis) with a mean of 0.66, while in the cultigens genetic diversity varied between 0.03 (locus SSR28) and 0.87 (locus SSR207) with a mean of 0.65. The cluster analysis indicated two major clusters, mainly one with the cultigens and the other with the wild accessions.

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Conservation and variability of sequence-tagged microsatellite sites (STMSs) from chickpea (Cicer aerietinum L.) within the genus Cicer

Cited by 88 publications

References 35 publications

Genetic diversity analysis among selected short duration chickpeacultivars and breeding lines based on STMS markers.

Genetic diversity analysis among selected short duration chickpeacultivars and breeding lines based on STMS markers.

Chickpea Improvement: Role of Wild Species and Genetic Markers

Development of new microsatellite markers and their application in the analysis of genetic diversity in lentils

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